Experiment: Temperature shift 10_to_30; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5241 and PP_5242 are separated by 239 nucleotides PP_5242 and PP_5243 are separated by 88 nucleotides PP_5243 and PP_5244 are separated by 71 nucleotides
PP_5241: PP_5241 - DNA-binding response regulator, LuxR family, at 5,978,124 to 5,979,116
_5241
PP_5242: PP_5242 - Sensor histidine kinase/GAF domain protein, at 5,979,356 to 5,984,404
_5242
PP_5243: PP_5243 - putative hydrolase, at 5,984,493 to 5,985,119
_5243
PP_5244: PP_5244 - Transcriptional regulator, AraC family, at 5,985,191 to 5,985,595
_5244
Position (kb) 5979
5980
5981
5982
5983
5984
5985 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5978.559 kb on + strand, within PP_5241 at 5978.559 kb on + strand, within PP_5241 at 5979.188 kb on + strand at 5979.188 kb on + strand at 5979.200 kb on + strand at 5979.200 kb on + strand at 5979.201 kb on - strand at 5979.219 kb on + strand at 5979.219 kb on + strand at 5979.219 kb on + strand at 5979.219 kb on + strand at 5979.220 kb on - strand at 5979.220 kb on - strand at 5979.220 kb on - strand at 5979.317 kb on + strand at 5979.319 kb on + strand at 5979.355 kb on + strand at 5979.355 kb on + strand at 5979.356 kb on - strand at 5979.356 kb on - strand at 5979.356 kb on - strand at 5979.393 kb on - strand at 5979.467 kb on + strand at 5979.468 kb on - strand at 5979.642 kb on - strand at 5979.770 kb on - strand at 5979.791 kb on + strand at 5979.791 kb on + strand at 5979.791 kb on + strand at 5979.792 kb on - strand at 5979.887 kb on + strand, within PP_5242 at 5979.919 kb on + strand, within PP_5242 at 5979.947 kb on + strand, within PP_5242 at 5979.947 kb on + strand, within PP_5242 at 5979.947 kb on + strand, within PP_5242 at 5979.947 kb on + strand, within PP_5242 at 5979.947 kb on + strand, within PP_5242 at 5979.948 kb on - strand, within PP_5242 at 5979.948 kb on - strand, within PP_5242 at 5979.948 kb on - strand, within PP_5242 at 5979.948 kb on - strand, within PP_5242 at 5979.968 kb on + strand, within PP_5242 at 5979.969 kb on - strand, within PP_5242 at 5979.975 kb on + strand, within PP_5242 at 5979.975 kb on + strand, within PP_5242 at 5979.975 kb on + strand, within PP_5242 at 5979.976 kb on - strand, within PP_5242 at 5979.981 kb on + strand, within PP_5242 at 5980.137 kb on - strand, within PP_5242 at 5980.288 kb on + strand, within PP_5242 at 5980.412 kb on + strand, within PP_5242 at 5980.458 kb on - strand, within PP_5242 at 5980.458 kb on - strand, within PP_5242 at 5980.476 kb on + strand, within PP_5242 at 5980.476 kb on + strand, within PP_5242 at 5980.476 kb on + strand, within PP_5242 at 5980.493 kb on - strand, within PP_5242 at 5980.513 kb on + strand, within PP_5242 at 5980.513 kb on + strand, within PP_5242 at 5980.514 kb on - strand, within PP_5242 at 5980.514 kb on - strand, within PP_5242 at 5980.630 kb on - strand, within PP_5242 at 5980.864 kb on - strand, within PP_5242 at 5981.121 kb on - strand, within PP_5242 at 5981.398 kb on + strand, within PP_5242 at 5981.399 kb on - strand, within PP_5242 at 5981.470 kb on - strand, within PP_5242 at 5981.792 kb on + strand, within PP_5242 at 5981.792 kb on + strand, within PP_5242 at 5981.793 kb on - strand, within PP_5242 at 5981.850 kb on + strand, within PP_5242 at 5981.850 kb on + strand, within PP_5242 at 5981.851 kb on - strand, within PP_5242 at 5981.856 kb on + strand, within PP_5242 at 5982.081 kb on + strand, within PP_5242 at 5982.110 kb on + strand, within PP_5242 at 5982.110 kb on + strand, within PP_5242 at 5982.125 kb on + strand, within PP_5242 at 5982.158 kb on + strand, within PP_5242 at 5982.158 kb on + strand, within PP_5242 at 5982.158 kb on + strand, within PP_5242 at 5982.353 kb on + strand, within PP_5242 at 5982.354 kb on - strand, within PP_5242 at 5982.354 kb on - strand, within PP_5242 at 5982.444 kb on + strand, within PP_5242 at 5982.445 kb on - strand, within PP_5242 at 5982.445 kb on - strand, within PP_5242 at 5982.602 kb on + strand, within PP_5242 at 5982.602 kb on + strand, within PP_5242 at 5982.602 kb on + strand, within PP_5242 at 5982.603 kb on - strand, within PP_5242 at 5982.614 kb on - strand, within PP_5242 at 5982.614 kb on - strand, within PP_5242 at 5982.692 kb on + strand, within PP_5242 at 5982.693 kb on - strand, within PP_5242 at 5982.838 kb on - strand, within PP_5242 at 5982.838 kb on - strand, within PP_5242 at 5983.118 kb on + strand, within PP_5242 at 5983.119 kb on - strand at 5983.272 kb on + strand, within PP_5242 at 5983.273 kb on - strand, within PP_5242 at 5983.273 kb on - strand, within PP_5242 at 5983.273 kb on - strand, within PP_5242 at 5983.273 kb on - strand, within PP_5242 at 5983.307 kb on + strand, within PP_5242 at 5983.536 kb on - strand, within PP_5242 at 5983.623 kb on + strand, within PP_5242 at 5983.624 kb on - strand, within PP_5242 at 5983.641 kb on - strand, within PP_5242 at 5984.110 kb on + strand at 5984.164 kb on + strand at 5984.248 kb on - strand at 5984.263 kb on + strand at 5984.263 kb on + strand at 5984.264 kb on - strand at 5984.305 kb on + strand at 5984.305 kb on + strand at 5984.306 kb on - strand at 5984.403 kb on - strand at 5984.403 kb on - strand at 5984.439 kb on + strand at 5984.451 kb on + strand at 5984.481 kb on + strand at 5984.481 kb on + strand at 5984.482 kb on - strand at 5984.482 kb on - strand at 5984.489 kb on + strand at 5984.489 kb on + strand at 5984.490 kb on - strand at 5984.515 kb on + strand at 5984.516 kb on - strand at 5984.658 kb on + strand, within PP_5243 at 5984.658 kb on + strand, within PP_5243 at 5984.875 kb on + strand, within PP_5243 at 5984.875 kb on + strand, within PP_5243 at 5984.876 kb on - strand, within PP_5243 at 5985.103 kb on + strand at 5985.103 kb on + strand at 5985.103 kb on + strand at 5985.171 kb on + strand at 5985.171 kb on + strand at 5985.182 kb on - strand at 5985.193 kb on - strand at 5985.308 kb on - strand, within PP_5244 at 5985.308 kb on - strand, within PP_5244
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_30; with MOPS remove 5,978,559 + PP_5241 0.44 -0.1 5,978,559 + PP_5241 0.44 +0.5 5,979,188 + +0.8 5,979,188 + +0.1 5,979,200 + -1.2 5,979,200 + +0.7 5,979,201 - -1.8 5,979,219 + +1.1 5,979,219 + +0.7 5,979,219 + +1.0 5,979,219 + -0.9 5,979,220 - +0.8 5,979,220 - +0.9 5,979,220 - +1.2 5,979,317 + +0.1 5,979,319 + -0.7 5,979,355 + -0.5 5,979,355 + -1.1 5,979,356 - -0.6 5,979,356 - +0.7 5,979,356 - -1.7 5,979,393 - +0.6 5,979,467 + +0.4 5,979,468 - -0.8 5,979,642 - -0.9 5,979,770 - +1.2 5,979,791 + +0.7 5,979,791 + -0.2 5,979,791 + -0.0 5,979,792 - -0.7 5,979,887 + PP_5242 0.11 -0.9 5,979,919 + PP_5242 0.11 +1.1 5,979,947 + PP_5242 0.12 +0.5 5,979,947 + PP_5242 0.12 -2.0 5,979,947 + PP_5242 0.12 -0.9 5,979,947 + PP_5242 0.12 -2.3 5,979,947 + PP_5242 0.12 -1.8 5,979,948 - PP_5242 0.12 +0.3 5,979,948 - PP_5242 0.12 -0.6 5,979,948 - PP_5242 0.12 -0.6 5,979,948 - PP_5242 0.12 +1.1 5,979,968 + PP_5242 0.12 -1.0 5,979,969 - PP_5242 0.12 -0.4 5,979,975 + PP_5242 0.12 -0.1 5,979,975 + PP_5242 0.12 +0.1 5,979,975 + PP_5242 0.12 +0.9 5,979,976 - PP_5242 0.12 -0.3 5,979,981 + PP_5242 0.12 -1.5 5,980,137 - PP_5242 0.15 +0.2 5,980,288 + PP_5242 0.18 -0.4 5,980,412 + PP_5242 0.21 -1.4 5,980,458 - PP_5242 0.22 -0.3 5,980,458 - PP_5242 0.22 +0.4 5,980,476 + PP_5242 0.22 -0.0 5,980,476 + PP_5242 0.22 -1.1 5,980,476 + PP_5242 0.22 -0.4 5,980,493 - PP_5242 0.23 -1.3 5,980,513 + PP_5242 0.23 -0.6 5,980,513 + PP_5242 0.23 +1.9 5,980,514 - PP_5242 0.23 -1.0 5,980,514 - PP_5242 0.23 +0.5 5,980,630 - PP_5242 0.25 -0.2 5,980,864 - PP_5242 0.30 +0.1 5,981,121 - PP_5242 0.35 -1.1 5,981,398 + PP_5242 0.40 -1.2 5,981,399 - PP_5242 0.40 -0.3 5,981,470 - PP_5242 0.42 -1.8 5,981,792 + PP_5242 0.48 +1.1 5,981,792 + PP_5242 0.48 -1.7 5,981,793 - PP_5242 0.48 -0.1 5,981,850 + PP_5242 0.49 -1.0 5,981,850 + PP_5242 0.49 -0.2 5,981,851 - PP_5242 0.49 -0.7 5,981,856 + PP_5242 0.50 -1.5 5,982,081 + PP_5242 0.54 +0.4 5,982,110 + PP_5242 0.55 +1.0 5,982,110 + PP_5242 0.55 -0.1 5,982,125 + PP_5242 0.55 -0.2 5,982,158 + PP_5242 0.55 +0.9 5,982,158 + PP_5242 0.55 +2.2 5,982,158 + PP_5242 0.55 -0.1 5,982,353 + PP_5242 0.59 +1.7 5,982,354 - PP_5242 0.59 -2.8 5,982,354 - PP_5242 0.59 +0.5 5,982,444 + PP_5242 0.61 -0.3 5,982,445 - PP_5242 0.61 +1.3 5,982,445 - PP_5242 0.61 +0.5 5,982,602 + PP_5242 0.64 -0.7 5,982,602 + PP_5242 0.64 +0.0 5,982,602 + PP_5242 0.64 -0.6 5,982,603 - PP_5242 0.64 -1.5 5,982,614 - PP_5242 0.65 +0.9 5,982,614 - PP_5242 0.65 -0.7 5,982,692 + PP_5242 0.66 +0.2 5,982,693 - PP_5242 0.66 -2.2 5,982,838 - PP_5242 0.69 -0.8 5,982,838 - PP_5242 0.69 +0.5 5,983,118 + PP_5242 0.75 -1.5 5,983,119 - +4.0 5,983,272 + PP_5242 0.78 -2.2 5,983,273 - PP_5242 0.78 +0.4 5,983,273 - PP_5242 0.78 -0.7 5,983,273 - PP_5242 0.78 +2.1 5,983,273 - PP_5242 0.78 -0.6 5,983,307 + PP_5242 0.78 -1.0 5,983,536 - PP_5242 0.83 +0.9 5,983,623 + PP_5242 0.85 -0.3 5,983,624 - PP_5242 0.85 -0.7 5,983,641 - PP_5242 0.85 -1.8 5,984,110 + +0.7 5,984,164 + +0.1 5,984,248 - +0.5 5,984,263 + -1.7 5,984,263 + -1.4 5,984,264 - +0.0 5,984,305 + +0.7 5,984,305 + +0.3 5,984,306 - -1.8 5,984,403 - +0.7 5,984,403 - -0.2 5,984,439 + -1.2 5,984,451 + +0.9 5,984,481 + +0.5 5,984,481 + -0.5 5,984,482 - -0.4 5,984,482 - -2.3 5,984,489 + -1.6 5,984,489 + -0.2 5,984,490 - +0.0 5,984,515 + +0.5 5,984,516 - -1.1 5,984,658 + PP_5243 0.26 +0.7 5,984,658 + PP_5243 0.26 +0.3 5,984,875 + PP_5243 0.61 +0.8 5,984,875 + PP_5243 0.61 +0.2 5,984,876 - PP_5243 0.61 -0.5 5,985,103 + -2.3 5,985,103 + +0.1 5,985,103 + +1.8 5,985,171 + +0.5 5,985,171 + +0.3 5,985,182 - +1.4 5,985,193 - -2.7 5,985,308 - PP_5244 0.29 +0.7 5,985,308 - PP_5244 0.29 +1.0
Or see this region's nucleotide sequence