Experiment: Temperature shift 10_to_30; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3868 and PP_3869 are separated by 469 nucleotides PP_3869 and PP_3870 overlap by 1 nucleotides PP_3870 and PP_3871 are separated by 5 nucleotides PP_3871 and PP_3873 are separated by 29 nucleotides
PP_3868: PP_3868 - Group II intron-encoding maturase, at 4,391,593 to 4,393,014
_3868
PP_3869: PP_3869 - putative Phage sheath protein, at 4,393,484 to 4,394,971
_3869
PP_3870: PP_3870 - Phage FluMu protein gp38, at 4,394,971 to 4,395,174
_3870
PP_3871: PP_3871 - conserved protein of unknown function, at 4,395,180 to 4,395,773
_3871
PP_3873: PP_3873 - conserved protein of unknown function, at 4,395,803 to 4,396,318
_3873
Position (kb)
4393
4394
4395 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4393.486 kb on - strand at 4393.515 kb on + strand at 4393.516 kb on - strand at 4393.516 kb on - strand at 4393.560 kb on + strand at 4393.561 kb on - strand at 4393.561 kb on - strand at 4393.611 kb on + strand at 4393.612 kb on - strand at 4393.662 kb on + strand, within PP_3869 at 4393.662 kb on + strand, within PP_3869 at 4393.662 kb on + strand, within PP_3869 at 4393.663 kb on - strand, within PP_3869 at 4393.663 kb on - strand, within PP_3869 at 4393.665 kb on + strand, within PP_3869 at 4393.762 kb on - strand, within PP_3869 at 4393.800 kb on + strand, within PP_3869 at 4393.800 kb on + strand, within PP_3869 at 4393.801 kb on - strand, within PP_3869 at 4393.914 kb on + strand, within PP_3869 at 4393.915 kb on - strand, within PP_3869 at 4394.115 kb on + strand, within PP_3869 at 4394.116 kb on - strand, within PP_3869 at 4394.116 kb on - strand, within PP_3869 at 4394.116 kb on - strand, within PP_3869 at 4394.190 kb on + strand, within PP_3869 at 4394.190 kb on + strand, within PP_3869 at 4394.190 kb on + strand, within PP_3869 at 4394.202 kb on + strand, within PP_3869 at 4394.202 kb on + strand, within PP_3869 at 4394.202 kb on + strand, within PP_3869 at 4394.203 kb on - strand, within PP_3869 at 4394.203 kb on - strand, within PP_3869 at 4394.616 kb on + strand, within PP_3869 at 4394.617 kb on - strand, within PP_3869 at 4394.617 kb on - strand, within PP_3869 at 4394.745 kb on + strand, within PP_3869 at 4394.745 kb on + strand, within PP_3869 at 4394.745 kb on + strand, within PP_3869 at 4394.745 kb on + strand at 4394.746 kb on - strand, within PP_3869 at 4394.746 kb on - strand, within PP_3869 at 4394.746 kb on - strand, within PP_3869 at 4394.746 kb on - strand, within PP_3869 at 4394.920 kb on - strand at 4394.972 kb on + strand at 4394.972 kb on + strand at 4394.972 kb on + strand at 4394.973 kb on - strand at 4394.973 kb on - strand at 4394.973 kb on - strand at 4394.973 kb on - strand at 4394.973 kb on - strand at 4394.989 kb on - strand at 4395.181 kb on + strand at 4395.319 kb on + strand, within PP_3871 at 4395.320 kb on - strand, within PP_3871 at 4395.394 kb on + strand, within PP_3871 at 4395.420 kb on + strand, within PP_3871 at 4395.420 kb on + strand, within PP_3871 at 4395.420 kb on + strand, within PP_3871 at 4395.421 kb on - strand, within PP_3871 at 4395.439 kb on + strand, within PP_3871 at 4395.440 kb on - strand, within PP_3871 at 4395.454 kb on + strand, within PP_3871 at 4395.649 kb on + strand, within PP_3871 at 4395.650 kb on - strand, within PP_3871 at 4395.958 kb on + strand, within PP_3873 at 4395.959 kb on - strand, within PP_3873 at 4395.959 kb on - strand, within PP_3873 at 4395.959 kb on - strand, within PP_3873 at 4395.959 kb on - strand, within PP_3873
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_30; with MOPS remove 4,393,486 - +1.6 4,393,515 + +0.6 4,393,516 - -1.4 4,393,516 - -0.7 4,393,560 + -0.7 4,393,561 - -0.0 4,393,561 - +1.0 4,393,611 + +0.7 4,393,612 - -1.4 4,393,662 + PP_3869 0.12 -0.5 4,393,662 + PP_3869 0.12 -0.1 4,393,662 + PP_3869 0.12 +0.9 4,393,663 - PP_3869 0.12 -0.1 4,393,663 - PP_3869 0.12 -2.0 4,393,665 + PP_3869 0.12 -1.7 4,393,762 - PP_3869 0.19 -1.2 4,393,800 + PP_3869 0.21 -1.0 4,393,800 + PP_3869 0.21 +0.4 4,393,801 - PP_3869 0.21 -0.3 4,393,914 + PP_3869 0.29 +0.5 4,393,915 - PP_3869 0.29 -1.0 4,394,115 + PP_3869 0.42 +0.7 4,394,116 - PP_3869 0.42 -2.3 4,394,116 - PP_3869 0.42 -0.1 4,394,116 - PP_3869 0.42 +1.0 4,394,190 + PP_3869 0.47 -0.9 4,394,190 + PP_3869 0.47 -1.0 4,394,190 + PP_3869 0.47 -2.6 4,394,202 + PP_3869 0.48 -1.2 4,394,202 + PP_3869 0.48 -0.3 4,394,202 + PP_3869 0.48 +0.3 4,394,203 - PP_3869 0.48 -0.7 4,394,203 - PP_3869 0.48 -1.8 4,394,616 + PP_3869 0.76 +1.3 4,394,617 - PP_3869 0.76 +1.8 4,394,617 - PP_3869 0.76 -0.1 4,394,745 + PP_3869 0.85 +0.1 4,394,745 + PP_3869 0.85 +0.8 4,394,745 + PP_3869 0.85 -1.4 4,394,745 + -0.7 4,394,746 - PP_3869 0.85 -0.0 4,394,746 - PP_3869 0.85 -0.3 4,394,746 - PP_3869 0.85 +0.3 4,394,746 - PP_3869 0.85 -0.7 4,394,920 - +0.7 4,394,972 + +0.8 4,394,972 + -2.9 4,394,972 + -0.5 4,394,973 - +0.3 4,394,973 - +1.2 4,394,973 - -1.1 4,394,973 - -1.0 4,394,973 - -0.2 4,394,989 - +0.3 4,395,181 + +0.2 4,395,319 + PP_3871 0.23 -0.1 4,395,320 - PP_3871 0.24 +0.7 4,395,394 + PP_3871 0.36 -1.5 4,395,420 + PP_3871 0.40 -0.5 4,395,420 + PP_3871 0.40 -0.3 4,395,420 + PP_3871 0.40 -0.5 4,395,421 - PP_3871 0.41 -0.0 4,395,439 + PP_3871 0.44 +0.3 4,395,440 - PP_3871 0.44 +1.0 4,395,454 + PP_3871 0.46 -2.1 4,395,649 + PP_3871 0.79 -2.6 4,395,650 - PP_3871 0.79 +0.2 4,395,958 + PP_3873 0.30 -1.3 4,395,959 - PP_3873 0.30 -1.5 4,395,959 - PP_3873 0.30 +1.3 4,395,959 - PP_3873 0.30 -0.1 4,395,959 - PP_3873 0.30 -0.9
Or see this region's nucleotide sequence