Strain Fitness in Pseudomonas putida KT2440 around PP_3977

Experiment: Temperature shift 10_to_30; with MOPS

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 10_to_30; with MOPS
4,483,873	+		PP_3976	0.30	-1.1
4,483,873	+		PP_3976	0.30	-0.2
4,483,873	+		PP_3976	0.30	-0.1
4,483,874	-		PP_3976	0.30	-0.5
4,483,874	-		PP_3976	0.30	-2.7
4,483,874	-		PP_3976	0.30	+1.1
4,483,878	-		PP_3976	0.30	+0.5
4,484,063	+		PP_3976	0.57	+0.3
4,484,063	+		PP_3976	0.57	-0.5
4,484,064	-		PP_3976	0.57	+0.3
4,484,064	-		PP_3976	0.57	-0.4
4,484,072	+		PP_3976	0.59	+0.1
4,484,072	+		PP_3976	0.59	-0.4
4,484,072	+		PP_3976	0.59	-0.2
4,484,072	+		PP_3976	0.59	-0.4
4,484,072	+		PP_3976	0.59	+0.1
4,484,072	+		PP_3976	0.59	-0.0
4,484,072	+		PP_3976	0.59	-0.0
4,484,072	+		PP_3976	0.59	-0.1
4,484,072	+		PP_3976	0.59	+0.4
4,484,072	+		PP_3976	0.59	+0.4
4,484,072	+		PP_3976	0.59	+0.4
4,484,072	+		PP_3976	0.59	+0.0
4,484,072	+		PP_3976	0.59	-0.7
4,484,072	+		PP_3976	0.59	+1.6
4,484,072	+		PP_3976	0.59	+0.5
4,484,073	-		PP_3976	0.59	-0.4
4,484,073	-		PP_3976	0.59	+0.2
4,484,073	-		PP_3976	0.59	-0.8
4,484,073	-		PP_3976	0.59	+0.1
4,484,073	-		PP_3976	0.59	-2.1
4,484,073	-		PP_3976	0.59	+1.2
4,484,073	-		PP_3976	0.59	-0.3
4,484,073	-		PP_3976	0.59	-1.6
4,484,073	-		PP_3976	0.59	+1.1
4,484,074	+		PP_3976	0.59	+0.2
4,484,074	+		PP_3976	0.59	-0.0
4,484,074	+		PP_3976	0.59	+0.3
4,484,075	-		PP_3976	0.59	+0.4
4,484,120	+		PP_3976	0.66	-0.0
4,484,121	-		PP_3976	0.66	+0.2
4,484,162	-		PP_3976	0.72	-0.1
4,484,178	+		PP_3976	0.74	+1.8
4,484,178	+		PP_3976	0.74	-0.5
4,484,179	-				+0.6
4,484,216	+		PP_3976	0.79	+0.4
4,484,216	+		PP_3976	0.79	-1.1
4,484,216	+		PP_3976	0.79	-0.5
4,484,216	+		PP_3976	0.79	-0.1
4,484,217	-		PP_3976	0.80	+0.2
4,484,217	-		PP_3976	0.80	+1.5
4,484,217	-		PP_3976	0.80	+0.1
4,484,276	+		PP_3976	0.88	-0.2
4,484,277	-		PP_3976	0.88	+1.3
4,484,282	+		PP_3976	0.89	-1.3
4,484,282	+		PP_3976	0.89	+0.9
4,484,282	+		PP_3976	0.89	-0.1
4,484,282	+		PP_3976	0.89	-0.9
4,484,282	+		PP_3976	0.89	-1.6
4,484,282	+		PP_3976	0.89	+1.4
4,484,283	-		PP_3976	0.89	+0.9
4,484,283	-		PP_3976	0.89	-1.3
4,484,283	-		PP_3976	0.89	+1.1
4,484,284	+		PP_3976	0.89	+1.2
4,484,284	+		PP_3976	0.89	-0.7
4,484,284	+		PP_3976	0.89	+0.2
4,484,284	+		PP_3976	0.89	+0.4
4,484,284	+		PP_3976	0.89	-2.2
4,484,285	-		PP_3976	0.90	-0.3
4,484,285	-		PP_3976	0.90	+1.3
4,484,487	+				-2.2
4,484,488	-				-0.5
4,484,488	-				+0.5
4,484,488	-				+0.5
4,484,545	+				+2.2
4,484,545	+				-0.2
4,484,545	+				-2.2
4,484,546	-				-0.3
4,484,548	-				+1.3
4,484,627	+				+0.3
4,484,676	+				-0.4
4,484,726	-				-0.5
4,484,784	-				-0.9
4,484,787	+				-1.7
4,484,788	-				-1.3
4,484,833	+				-0.7
4,484,834	-				-0.1
4,484,834	-				-1.8
4,484,834	-				-0.8
4,484,898	+				-0.8
4,484,898	+				-1.1
4,484,898	+				+0.5
4,484,899	-				+1.4
4,484,899	-				+0.6
4,484,928	+				+2.3
4,484,928	+				-0.7
4,485,108	+		PP_3977	0.22	-2.2
4,485,109	-		PP_3977	0.22	-0.5
4,485,157	+		PP_3977	0.25	-1.5
4,485,157	+		PP_3977	0.25	-0.2
4,485,158	-				+2.6
4,485,189	+		PP_3977	0.28	-0.4
4,485,189	+		PP_3977	0.28	+0.7
4,485,190	-		PP_3977	0.28	+0.2
4,485,190	-		PP_3977	0.28	+1.1
4,485,190	-		PP_3977	0.28	+0.9
4,485,199	+		PP_3977	0.29	+0.1
4,485,199	+		PP_3977	0.29	+0.8
4,485,200	-		PP_3977	0.29	-1.4
4,485,207	+		PP_3977	0.29	+0.7
4,485,208	-		PP_3977	0.29	-0.2
4,485,215	+		PP_3977	0.30	+0.8
4,485,216	-		PP_3977	0.30	-0.2
4,485,216	-		PP_3977	0.30	-0.9
4,485,216	-		PP_3977	0.30	+0.8
4,485,216	-		PP_3977	0.30	+0.9
4,485,237	+		PP_3977	0.32	+0.9
4,485,238	-		PP_3977	0.32	+1.1
4,485,277	-		PP_3977	0.35	+0.7
4,485,277	-		PP_3977	0.35	-0.6
4,485,277	-		PP_3977	0.35	+1.0
4,485,310	-		PP_3977	0.37	-0.8
4,485,318	+		PP_3977	0.38	-0.2
4,485,318	+		PP_3977	0.38	-0.2
4,485,318	+		PP_3977	0.38	+0.3
4,485,407	+		PP_3977	0.45	-0.1
4,485,407	+		PP_3977	0.45	-0.8
4,485,408	-		PP_3977	0.45	+0.1
4,485,429	+		PP_3977	0.46	-2.4
4,485,429	+		PP_3977	0.46	+0.1
4,485,430	-		PP_3977	0.46	-0.1
4,485,430	-		PP_3977	0.46	+0.9
4,485,430	-		PP_3977	0.46	+1.5
4,485,456	-		PP_3977	0.48	-1.5
4,485,464	+		PP_3977	0.49	+0.8
4,485,464	+		PP_3977	0.49	-0.3
4,485,465	-		PP_3977	0.49	+1.6
4,485,496	+		PP_3977	0.52	+0.4
4,485,524	+		PP_3977	0.54	-1.2
4,485,525	-		PP_3977	0.54	+0.7
4,485,525	-		PP_3977	0.54	+0.6
4,485,590	+		PP_3977	0.59	+1.0
4,485,591	-		PP_3977	0.59	+0.4
4,485,630	-		PP_3977	0.62	+0.0
4,485,690	+		PP_3977	0.66	+2.0
4,485,690	+		PP_3977	0.66	-0.0
4,485,691	-		PP_3977	0.67	-1.5
4,485,768	+		PP_3977	0.72	-0.1
4,485,780	+		PP_3977	0.73	+1.9
4,485,780	+		PP_3977	0.73	+0.5
4,485,780	+		PP_3977	0.73	-0.2
4,485,780	+				+1.6
4,485,781	-		PP_3977	0.73	+0.4
4,485,781	-		PP_3977	0.73	+0.3
4,485,781	-		PP_3977	0.73	+1.0
4,485,799	-		PP_3977	0.75	+0.3
4,485,799	-		PP_3977	0.75	-0.9
4,485,903	+		PP_3977	0.83	+0.1
4,485,904	-		PP_3977	0.83	-0.4
4,485,962	+		PP_3977	0.87	+1.5
4,485,962	+		PP_3977	0.87	+0.3
4,485,963	-		PP_3977	0.87	+0.8
4,485,963	-		PP_3977	0.87	-1.1
4,485,970	+		PP_3977	0.88	+0.4
4,485,970	+		PP_3977	0.88	+0.5
4,485,970	+		PP_3977	0.88	-0.7
4,485,970	+		PP_3977	0.88	-1.5
4,485,970	+		PP_3977	0.88	-1.9
4,485,970	+		PP_3977	0.88	+0.9
4,485,970	+		PP_3977	0.88	+0.7
4,485,970	+		PP_3977	0.88	-0.4
4,485,970	+		PP_3977	0.88	-0.4
4,485,970	+		PP_3977	0.88	+0.5
4,485,971	-		PP_3977	0.88	+0.8
4,485,971	-		PP_3977	0.88	+1.5
4,485,971	-		PP_3977	0.88	+2.1
4,485,971	-		PP_3977	0.88	+1.1
4,485,971	-		PP_3977	0.88	-2.5
4,485,974	+		PP_3977	0.88	+0.1
4,485,975	-		PP_3977	0.88	-0.8
4,485,975	-		PP_3977	0.88	-1.9
4,485,975	-		PP_3977	0.88	+0.2
4,486,019	-				-0.4
4,486,028	+				-0.8
4,486,028	+				-0.0
4,486,033	+				+0.4
4,486,034	-				+1.1
4,486,034	-				+0.0
4,486,042	+				-0.9
4,486,092	-				+0.0
4,486,097	+				-0.3
4,486,097	+				+0.0
4,486,097	+				-0.3
4,486,097	+				+0.6
4,486,097	+				-0.7
4,486,097	+				+0.9
4,486,097	+				+1.1
4,486,097	+				-0.2
4,486,097	+				-1.1
4,486,097	+				-0.6
4,486,098	-				+1.1
4,486,098	-				+1.8
4,486,124	+				+0.4
4,486,124	+				+0.1
4,486,124	+				+0.6
4,486,124	+				+0.1
4,486,125	-				+0.7
4,486,125	-				-0.8
4,486,125	-				-0.8
4,486,128	+				-1.8
4,486,128	+				+0.4
4,486,128	+				+0.1
4,486,128	+				+0.4
4,486,128	+				+0.1
4,486,128	+				-0.2
4,486,128	+				-0.3
4,486,128	+				+0.3
4,486,128	+				-0.1
4,486,128	+				-0.5
4,486,128	+				-1.2
4,486,128	+				+0.5
4,486,128	+				-1.3
4,486,128	+				-0.3
4,486,128	+				-0.6
4,486,128	+				-0.1
4,486,129	-				+0.5
4,486,129	-				-0.4
4,486,129	-				+0.1
4,486,129	-				-1.8
4,486,129	-				-1.0
4,486,129	-				+1.6
4,486,129	-				-1.1
4,486,129	-				+0.2
4,486,129	-				-0.2
4,486,129	-				+0.8
4,486,129	-				-0.6
4,486,177	+		PP_3978	0.28	-0.1
4,486,177	+		PP_3978	0.28	+0.8
4,486,177	+		PP_3978	0.28	-2.8
4,486,177	+		PP_3978	0.28	-0.1
4,486,178	-		PP_3978	0.29	-0.5
4,486,178	-		PP_3978	0.29	-0.9
4,486,178	-		PP_3978	0.29	+0.6
4,486,178	-		PP_3978	0.29	-0.3
4,486,178	-		PP_3978	0.29	-0.9
4,486,260	+		PP_3978	0.77	-0.7
4,486,288	+				+0.9
4,486,288	+				+0.3
4,486,288	+				+0.7
4,486,335	-				+0.1