Experiment: Temperature shift 10_to_30; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2752 and PP_2753 are separated by 116 nucleotides PP_2753 and PP_2754 are separated by 289 nucleotides
PP_2752: PP_2752 - conserved exported protein of unknown function, at 3,135,348 to 3,136,682
_2752
PP_2753: PP_2753 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,136,799 to 3,137,572
_2753
PP_2754: PP_2754 - putative OprD family outer membrane porin, at 3,137,862 to 3,139,211
_2754
Position (kb)
3136
3137
3138 Strain fitness (log2 ratio)
-2
-1
0
1 at 3135.840 kb on + strand, within PP_2752 at 3135.841 kb on - strand, within PP_2752 at 3135.961 kb on - strand, within PP_2752 at 3136.197 kb on + strand, within PP_2752 at 3136.317 kb on + strand, within PP_2752 at 3136.318 kb on - strand, within PP_2752 at 3136.320 kb on + strand, within PP_2752 at 3136.320 kb on + strand, within PP_2752 at 3136.320 kb on + strand, within PP_2752 at 3136.790 kb on + strand at 3136.790 kb on + strand at 3136.791 kb on - strand at 3136.794 kb on - strand at 3136.939 kb on - strand, within PP_2753 at 3137.210 kb on + strand, within PP_2753 at 3137.210 kb on + strand, within PP_2753 at 3137.211 kb on - strand, within PP_2753 at 3137.211 kb on - strand, within PP_2753 at 3137.474 kb on + strand, within PP_2753 at 3137.474 kb on + strand, within PP_2753 at 3137.475 kb on - strand, within PP_2753 at 3137.475 kb on - strand, within PP_2753 at 3137.475 kb on - strand, within PP_2753 at 3137.500 kb on - strand at 3137.540 kb on - strand at 3137.619 kb on + strand at 3137.620 kb on - strand at 3137.626 kb on + strand at 3137.736 kb on + strand at 3137.737 kb on - strand at 3137.836 kb on - strand at 3138.259 kb on - strand, within PP_2754 at 3138.507 kb on - strand, within PP_2754 at 3138.532 kb on - strand, within PP_2754 at 3138.532 kb on - strand, within PP_2754
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_30; with MOPS remove 3,135,840 + PP_2752 0.37 -2.6 3,135,841 - PP_2752 0.37 +1.2 3,135,961 - PP_2752 0.46 -0.2 3,136,197 + PP_2752 0.64 +0.5 3,136,317 + PP_2752 0.73 +0.4 3,136,318 - PP_2752 0.73 +0.1 3,136,320 + PP_2752 0.73 +0.1 3,136,320 + PP_2752 0.73 +0.5 3,136,320 + PP_2752 0.73 -0.6 3,136,790 + -1.8 3,136,790 + +0.7 3,136,791 - -0.7 3,136,794 - -1.0 3,136,939 - PP_2753 0.18 -0.6 3,137,210 + PP_2753 0.53 +0.1 3,137,210 + PP_2753 0.53 -1.8 3,137,211 - PP_2753 0.53 -1.0 3,137,211 - PP_2753 0.53 -1.1 3,137,474 + PP_2753 0.87 -2.1 3,137,474 + PP_2753 0.87 -0.0 3,137,475 - PP_2753 0.87 -0.6 3,137,475 - PP_2753 0.87 -1.1 3,137,475 - PP_2753 0.87 -1.0 3,137,500 - +0.3 3,137,540 - +0.3 3,137,619 + -0.2 3,137,620 - -0.2 3,137,626 + -1.3 3,137,736 + +0.1 3,137,737 - -1.1 3,137,836 - -0.1 3,138,259 - PP_2754 0.29 +1.4 3,138,507 - PP_2754 0.48 -1.0 3,138,532 - PP_2754 0.50 -1.2 3,138,532 - PP_2754 0.50 +1.0
Or see this region's nucleotide sequence