Experiment: Temperature shift 30_to_21; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5023 and PP_5024 are separated by 79 nucleotides PP_5024 and PP_5025 are separated by 293 nucleotides
PP_5023: PP_5023 - Amino acid ABC transporter, permease protein, at 5,722,256 to 5,723,218
_5023
PP_5024: PP_5024 - Amino acid ABC transporter, periplasmic amino acid-binding protein, at 5,723,298 to 5,724,098
_5024
PP_5025: PP_5025 - Glucans biosynthesis glucosyltransferase H, at 5,724,392 to 5,726,965
_5025
Position (kb) 5723
5724
5725 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5722.326 kb on + strand at 5722.326 kb on + strand at 5722.327 kb on - strand at 5722.327 kb on - strand at 5722.327 kb on - strand at 5722.361 kb on - strand, within PP_5023 at 5722.379 kb on - strand, within PP_5023 at 5722.379 kb on - strand, within PP_5023 at 5722.608 kb on + strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.686 kb on + strand, within PP_5023 at 5722.686 kb on + strand, within PP_5023 at 5722.687 kb on - strand, within PP_5023 at 5722.687 kb on - strand, within PP_5023 at 5722.687 kb on - strand, within PP_5023 at 5722.738 kb on - strand, within PP_5023 at 5723.043 kb on + strand, within PP_5023 at 5723.077 kb on - strand, within PP_5023 at 5723.115 kb on + strand, within PP_5023 at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.140 kb on - strand at 5723.140 kb on - strand at 5723.140 kb on - strand at 5723.225 kb on + strand at 5723.233 kb on + strand at 5723.234 kb on - strand at 5723.274 kb on - strand at 5723.274 kb on - strand at 5723.299 kb on + strand at 5723.300 kb on - strand at 5723.348 kb on + strand at 5723.356 kb on + strand at 5723.356 kb on + strand at 5723.356 kb on + strand at 5723.357 kb on - strand at 5723.357 kb on - strand at 5723.414 kb on - strand, within PP_5024 at 5723.449 kb on + strand, within PP_5024 at 5723.516 kb on - strand, within PP_5024 at 5723.527 kb on + strand, within PP_5024 at 5723.597 kb on - strand, within PP_5024 at 5723.665 kb on + strand, within PP_5024 at 5723.689 kb on + strand, within PP_5024 at 5723.689 kb on + strand, within PP_5024 at 5723.689 kb on + strand, within PP_5024 at 5723.690 kb on - strand, within PP_5024 at 5723.854 kb on + strand, within PP_5024 at 5723.968 kb on + strand, within PP_5024 at 5723.968 kb on + strand, within PP_5024 at 5723.968 kb on + strand, within PP_5024 at 5723.969 kb on - strand, within PP_5024 at 5723.969 kb on - strand, within PP_5024 at 5723.969 kb on - strand, within PP_5024 at 5724.086 kb on - strand at 5724.133 kb on + strand at 5724.134 kb on - strand at 5724.134 kb on - strand at 5724.134 kb on - strand at 5724.134 kb on - strand at 5724.185 kb on + strand at 5724.185 kb on + strand at 5724.281 kb on + strand at 5724.282 kb on - strand at 5724.282 kb on - strand at 5724.295 kb on + strand at 5724.296 kb on - strand at 5724.305 kb on - strand at 5724.571 kb on + strand at 5724.759 kb on + strand, within PP_5025 at 5724.759 kb on + strand, within PP_5025 at 5724.760 kb on - strand, within PP_5025 at 5724.796 kb on - strand, within PP_5025
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_21; with MOPS remove 5,722,326 + +0.1 5,722,326 + -2.6 5,722,327 - -2.9 5,722,327 - +0.5 5,722,327 - +0.5 5,722,361 - PP_5023 0.11 -0.2 5,722,379 - PP_5023 0.13 +0.1 5,722,379 - PP_5023 0.13 +0.5 5,722,608 + PP_5023 0.37 -1.2 5,722,609 - PP_5023 0.37 +1.3 5,722,609 - PP_5023 0.37 +0.8 5,722,609 - PP_5023 0.37 -2.1 5,722,609 - PP_5023 0.37 -0.4 5,722,609 - PP_5023 0.37 +0.3 5,722,686 + PP_5023 0.45 -0.6 5,722,686 + PP_5023 0.45 +1.4 5,722,687 - PP_5023 0.45 -1.8 5,722,687 - PP_5023 0.45 -1.0 5,722,687 - PP_5023 0.45 +1.0 5,722,738 - PP_5023 0.50 +1.8 5,723,043 + PP_5023 0.82 +0.2 5,723,077 - PP_5023 0.85 -3.3 5,723,115 + PP_5023 0.89 +0.2 5,723,139 + -0.4 5,723,139 + +1.0 5,723,139 + +0.6 5,723,139 + +0.9 5,723,139 + -4.1 5,723,139 + -3.3 5,723,140 - +0.1 5,723,140 - +1.0 5,723,140 - +0.7 5,723,225 + -3.3 5,723,233 + -0.0 5,723,234 - -0.1 5,723,274 - -0.0 5,723,274 - -0.2 5,723,299 + -1.3 5,723,300 - +0.2 5,723,348 + +1.7 5,723,356 + +1.0 5,723,356 + +0.6 5,723,356 + -2.9 5,723,357 - -1.1 5,723,357 - -4.0 5,723,414 - PP_5024 0.14 +0.2 5,723,449 + PP_5024 0.19 +0.9 5,723,516 - PP_5024 0.27 +0.6 5,723,527 + PP_5024 0.29 -0.8 5,723,597 - PP_5024 0.37 +0.0 5,723,665 + PP_5024 0.46 -3.4 5,723,689 + PP_5024 0.49 +0.1 5,723,689 + PP_5024 0.49 +0.0 5,723,689 + PP_5024 0.49 -3.7 5,723,690 - PP_5024 0.49 -2.8 5,723,854 + PP_5024 0.69 -0.0 5,723,968 + PP_5024 0.84 -2.4 5,723,968 + PP_5024 0.84 -1.0 5,723,968 + PP_5024 0.84 +0.1 5,723,969 - PP_5024 0.84 -1.1 5,723,969 - PP_5024 0.84 -1.4 5,723,969 - PP_5024 0.84 -1.5 5,724,086 - +1.3 5,724,133 + -0.5 5,724,134 - -0.6 5,724,134 - -0.7 5,724,134 - -3.7 5,724,134 - -0.7 5,724,185 + +1.1 5,724,185 + +0.6 5,724,281 + +0.7 5,724,282 - +2.3 5,724,282 - -0.5 5,724,295 + +0.0 5,724,296 - -0.2 5,724,305 - +0.4 5,724,571 + -3.6 5,724,759 + PP_5025 0.14 -0.1 5,724,759 + PP_5025 0.14 -0.8 5,724,760 - PP_5025 0.14 -2.1 5,724,796 - PP_5025 0.16 -1.4
Or see this region's nucleotide sequence