Experiment: Temperature shift 30_to_21; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3964 and PP_3965 are separated by 63 nucleotides PP_3965 and PP_3966 overlap by 4 nucleotides PP_3966 and PP_3967 are separated by 175 nucleotides PP_3967 and PP_3968 are separated by 5 nucleotides
PP_3964: PP_3964 - transposase, at 4,474,000 to 4,475,535
_3964
PP_3965: PP_3965 - transposase, at 4,475,599 to 4,475,934
_3965
PP_3966: PP_3966 - transposase, at 4,475,931 to 4,476,251
_3966
PP_3967: PP_3967 - MutT/nudix family protein, at 4,476,427 to 4,476,867
_3967
PP_3968: PP_3968 - Sensor histidine kinase, at 4,476,873 to 4,477,991
_3968
Position (kb) 4476
4477 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4475.600 kb on + strand at 4475.611 kb on - strand at 4475.611 kb on - strand at 4475.816 kb on + strand, within PP_3965 at 4476.434 kb on + strand at 4476.434 kb on + strand at 4476.434 kb on + strand at 4476.435 kb on - strand at 4476.695 kb on - strand, within PP_3967 at 4476.720 kb on - strand, within PP_3967 at 4476.720 kb on - strand, within PP_3967 at 4476.798 kb on + strand, within PP_3967 at 4476.799 kb on - strand, within PP_3967 at 4476.833 kb on + strand at 4476.834 kb on - strand at 4476.834 kb on - strand at 4476.834 kb on - strand at 4476.846 kb on + strand at 4476.927 kb on + strand at 4476.928 kb on - strand at 4476.928 kb on - strand at 4476.928 kb on - strand at 4476.969 kb on + strand at 4476.970 kb on - strand at 4476.982 kb on - strand at 4477.016 kb on - strand, within PP_3968 at 4477.016 kb on - strand, within PP_3968 at 4477.025 kb on - strand, within PP_3968 at 4477.230 kb on + strand, within PP_3968 at 4477.248 kb on - strand, within PP_3968 at 4477.338 kb on - strand, within PP_3968 at 4477.385 kb on + strand, within PP_3968 at 4477.385 kb on + strand, within PP_3968 at 4477.386 kb on - strand, within PP_3968 at 4477.386 kb on - strand, within PP_3968 at 4477.409 kb on + strand, within PP_3968 at 4477.468 kb on - strand, within PP_3968 at 4477.485 kb on + strand, within PP_3968 at 4477.485 kb on + strand, within PP_3968 at 4477.486 kb on - strand, within PP_3968 at 4477.530 kb on + strand, within PP_3968 at 4477.564 kb on + strand, within PP_3968 at 4477.565 kb on - strand, within PP_3968 at 4477.671 kb on + strand, within PP_3968 at 4477.671 kb on + strand, within PP_3968 at 4477.672 kb on - strand, within PP_3968 at 4477.731 kb on + strand, within PP_3968 at 4477.742 kb on + strand, within PP_3968 at 4477.780 kb on - strand, within PP_3968 at 4477.780 kb on - strand, within PP_3968 at 4477.832 kb on + strand, within PP_3968 at 4477.833 kb on - strand, within PP_3968 at 4477.833 kb on - strand, within PP_3968 at 4477.839 kb on - strand, within PP_3968 at 4477.839 kb on - strand, within PP_3968
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_21; with MOPS remove 4,475,600 + -2.4 4,475,611 - -2.1 4,475,611 - +0.6 4,475,816 + PP_3965 0.65 +2.0 4,476,434 + +0.7 4,476,434 + -1.6 4,476,434 + -0.7 4,476,435 - -1.1 4,476,695 - PP_3967 0.61 -1.5 4,476,720 - PP_3967 0.66 +0.3 4,476,720 - PP_3967 0.66 -4.1 4,476,798 + PP_3967 0.84 -0.6 4,476,799 - PP_3967 0.84 +0.1 4,476,833 + -0.0 4,476,834 - -3.0 4,476,834 - +0.9 4,476,834 - -3.5 4,476,846 + +3.1 4,476,927 + +0.5 4,476,928 - -0.8 4,476,928 - +1.6 4,476,928 - -2.8 4,476,969 + +1.1 4,476,970 - +0.9 4,476,982 - -3.8 4,477,016 - PP_3968 0.13 +1.3 4,477,016 - PP_3968 0.13 +0.6 4,477,025 - PP_3968 0.14 +1.2 4,477,230 + PP_3968 0.32 -0.6 4,477,248 - PP_3968 0.34 -0.2 4,477,338 - PP_3968 0.42 -0.4 4,477,385 + PP_3968 0.46 -0.8 4,477,385 + PP_3968 0.46 +1.3 4,477,386 - PP_3968 0.46 +1.5 4,477,386 - PP_3968 0.46 +0.7 4,477,409 + PP_3968 0.48 -0.6 4,477,468 - PP_3968 0.53 +0.9 4,477,485 + PP_3968 0.55 -0.3 4,477,485 + PP_3968 0.55 +1.0 4,477,486 - PP_3968 0.55 -3.5 4,477,530 + PP_3968 0.59 -4.5 4,477,564 + PP_3968 0.62 +1.3 4,477,565 - PP_3968 0.62 +0.1 4,477,671 + PP_3968 0.71 +0.3 4,477,671 + PP_3968 0.71 +0.4 4,477,672 - PP_3968 0.71 +1.3 4,477,731 + PP_3968 0.77 +0.6 4,477,742 + PP_3968 0.78 +0.4 4,477,780 - PP_3968 0.81 +1.5 4,477,780 - PP_3968 0.81 +1.3 4,477,832 + PP_3968 0.86 +0.4 4,477,833 - PP_3968 0.86 +0.2 4,477,833 - PP_3968 0.86 +1.2 4,477,839 - PP_3968 0.86 -3.2 4,477,839 - PP_3968 0.86 +0.1
Or see this region's nucleotide sequence