Experiment: Temperature shift 30_to_21; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3261 and PP_3262 overlap by 4 nucleotides PP_3262 and PP_5595 are separated by 91 nucleotides PP_5595 and PP_3263 are separated by 28 nucleotides PP_3263 and PP_3264 overlap by 1 nucleotides
PP_3261: PP_3261 - conserved protein of unknown function, at 3,696,358 to 3,696,759
_3261
PP_3262: PP_3262 - putative Metallothionein, at 3,696,756 to 3,696,980
_3262
PP_5595: PP_5595 - conserved exported protein of unknown function, at 3,697,072 to 3,697,488
_5595
PP_3263: PP_3263 - phospholipid modification enzyme, at 3,697,517 to 3,698,470
_3263
PP_3264: PP_3264 - cardiolipin synthase 2, at 3,698,470 to 3,699,672
_3264
Position (kb)
3697
3698
3699 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3696.834 kb on + strand, within PP_3262 at 3696.835 kb on - strand, within PP_3262 at 3696.988 kb on + strand at 3697.030 kb on + strand at 3697.031 kb on - strand at 3697.143 kb on - strand, within PP_5595 at 3697.143 kb on - strand, within PP_5595 at 3697.143 kb on - strand, within PP_5595 at 3697.145 kb on + strand, within PP_5595 at 3697.145 kb on + strand at 3697.145 kb on + strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.355 kb on + strand, within PP_5595 at 3697.355 kb on + strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.579 kb on - strand at 3697.579 kb on - strand at 3697.611 kb on + strand at 3697.611 kb on + strand at 3697.614 kb on + strand, within PP_3263 at 3697.614 kb on + strand, within PP_3263 at 3697.615 kb on - strand, within PP_3263 at 3697.615 kb on - strand, within PP_3263 at 3697.777 kb on - strand, within PP_3263 at 3697.777 kb on - strand, within PP_3263 at 3697.941 kb on + strand, within PP_3263 at 3697.991 kb on - strand, within PP_3263 at 3698.039 kb on + strand, within PP_3263 at 3698.039 kb on + strand, within PP_3263 at 3698.039 kb on + strand at 3698.059 kb on + strand, within PP_3263 at 3698.059 kb on + strand, within PP_3263 at 3698.059 kb on + strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.061 kb on + strand, within PP_3263 at 3698.062 kb on - strand, within PP_3263 at 3698.062 kb on - strand, within PP_3263 at 3698.196 kb on + strand, within PP_3263 at 3698.219 kb on + strand, within PP_3263 at 3698.234 kb on + strand, within PP_3263 at 3698.240 kb on - strand, within PP_3263 at 3698.240 kb on - strand, within PP_3263 at 3698.271 kb on + strand, within PP_3263 at 3698.271 kb on + strand, within PP_3263 at 3698.289 kb on + strand, within PP_3263 at 3698.289 kb on + strand, within PP_3263 at 3698.290 kb on - strand, within PP_3263 at 3698.290 kb on - strand, within PP_3263 at 3698.337 kb on + strand, within PP_3263 at 3698.338 kb on - strand, within PP_3263 at 3698.392 kb on - strand at 3698.837 kb on + strand, within PP_3264 at 3698.838 kb on - strand, within PP_3264 at 3698.870 kb on + strand, within PP_3264 at 3698.871 kb on - strand at 3698.871 kb on - strand, within PP_3264 at 3698.876 kb on + strand, within PP_3264 at 3698.877 kb on - strand, within PP_3264 at 3699.053 kb on + strand, within PP_3264 at 3699.053 kb on + strand, within PP_3264 at 3699.054 kb on - strand, within PP_3264 at 3699.054 kb on - strand, within PP_3264 at 3699.234 kb on - strand, within PP_3264 at 3699.266 kb on - strand, within PP_3264 at 3699.282 kb on - strand, within PP_3264
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_21; with MOPS remove 3,696,834 + PP_3262 0.35 -0.2 3,696,835 - PP_3262 0.35 +2.0 3,696,988 + -0.1 3,697,030 + +0.7 3,697,031 - -2.8 3,697,143 - PP_5595 0.17 -0.4 3,697,143 - PP_5595 0.17 -1.2 3,697,143 - PP_5595 0.17 -0.9 3,697,145 + PP_5595 0.18 -2.5 3,697,145 + +0.8 3,697,145 + PP_5595 0.18 -2.5 3,697,146 - PP_5595 0.18 +0.7 3,697,146 - +3.5 3,697,146 - PP_5595 0.18 -3.6 3,697,146 - PP_5595 0.18 +0.5 3,697,146 - PP_5595 0.18 -3.4 3,697,146 - PP_5595 0.18 +0.7 3,697,355 + PP_5595 0.68 +0.7 3,697,355 + PP_5595 0.68 -0.6 3,697,362 - PP_5595 0.70 -3.0 3,697,362 - PP_5595 0.70 -3.8 3,697,362 - PP_5595 0.70 +1.7 3,697,362 - PP_5595 0.70 +0.5 3,697,579 - +1.5 3,697,579 - +1.8 3,697,611 + -0.6 3,697,611 + +0.1 3,697,614 + PP_3263 0.10 +1.8 3,697,614 + PP_3263 0.10 -0.1 3,697,615 - PP_3263 0.10 +1.6 3,697,615 - PP_3263 0.10 -3.3 3,697,777 - PP_3263 0.27 +0.2 3,697,777 - PP_3263 0.27 +0.3 3,697,941 + PP_3263 0.44 +0.9 3,697,991 - PP_3263 0.50 +1.6 3,698,039 + PP_3263 0.55 +1.4 3,698,039 + PP_3263 0.55 +0.8 3,698,039 + +2.6 3,698,059 + PP_3263 0.57 -3.6 3,698,059 + PP_3263 0.57 +0.7 3,698,059 + PP_3263 0.57 -1.7 3,698,060 - PP_3263 0.57 -0.4 3,698,060 - PP_3263 0.57 +0.1 3,698,060 - PP_3263 0.57 +1.8 3,698,060 - PP_3263 0.57 -0.6 3,698,061 + PP_3263 0.57 -0.0 3,698,062 - PP_3263 0.57 +1.6 3,698,062 - PP_3263 0.57 +0.1 3,698,196 + PP_3263 0.71 +0.6 3,698,219 + PP_3263 0.74 +2.9 3,698,234 + PP_3263 0.75 +0.1 3,698,240 - PP_3263 0.76 +0.4 3,698,240 - PP_3263 0.76 +1.6 3,698,271 + PP_3263 0.79 -0.4 3,698,271 + PP_3263 0.79 +0.5 3,698,289 + PP_3263 0.81 -3.8 3,698,289 + PP_3263 0.81 +1.2 3,698,290 - PP_3263 0.81 +1.4 3,698,290 - PP_3263 0.81 -0.8 3,698,337 + PP_3263 0.86 +1.0 3,698,338 - PP_3263 0.86 +0.7 3,698,392 - +0.3 3,698,837 + PP_3264 0.31 +0.7 3,698,838 - PP_3264 0.31 -0.9 3,698,870 + PP_3264 0.33 -2.5 3,698,871 - -0.2 3,698,871 - PP_3264 0.33 -0.3 3,698,876 + PP_3264 0.34 -0.8 3,698,877 - PP_3264 0.34 -0.2 3,699,053 + PP_3264 0.48 -0.6 3,699,053 + PP_3264 0.48 +0.8 3,699,054 - PP_3264 0.49 -1.5 3,699,054 - PP_3264 0.49 +0.7 3,699,234 - PP_3264 0.64 +0.1 3,699,266 - PP_3264 0.66 -2.3 3,699,282 - PP_3264 0.67 -0.0
Or see this region's nucleotide sequence