Experiment: Temperature shift 30_to_21; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3723 and PP_3724 overlap by 8 nucleotides PP_3724 and PP_3725 are separated by 17 nucleotides
PP_3723: PP_3723 - putative 2-ketoarginine decarboxylase AruI, at 4,246,309 to 4,247,916
_3723
PP_3724: PP_3724 - putative Acyl-CoA synthetase, at 4,247,909 to 4,250,026
_3724
PP_3725: PP_3725 - putative Acyl-CoA dehydrogenase, at 4,250,044 to 4,251,204
_3725
Position (kb)
4247
4248
4249
4250
4251 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4247.289 kb on + strand, within PP_3723 at 4247.290 kb on - strand, within PP_3723 at 4247.369 kb on - strand, within PP_3723 at 4247.369 kb on - strand, within PP_3723 at 4247.776 kb on + strand at 4247.776 kb on + strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.810 kb on + strand at 4247.811 kb on - strand at 4247.862 kb on - strand at 4247.862 kb on - strand at 4247.862 kb on - strand at 4247.862 kb on - strand at 4247.969 kb on - strand at 4248.037 kb on - strand at 4248.037 kb on - strand at 4248.266 kb on + strand, within PP_3724 at 4248.267 kb on - strand, within PP_3724 at 4248.267 kb on - strand, within PP_3724 at 4248.336 kb on + strand, within PP_3724 at 4248.336 kb on + strand, within PP_3724 at 4248.337 kb on - strand, within PP_3724 at 4248.337 kb on - strand, within PP_3724 at 4248.459 kb on - strand, within PP_3724 at 4248.509 kb on - strand, within PP_3724 at 4248.667 kb on - strand, within PP_3724 at 4248.826 kb on - strand, within PP_3724 at 4249.003 kb on - strand, within PP_3724 at 4249.237 kb on - strand, within PP_3724 at 4249.488 kb on + strand, within PP_3724 at 4249.488 kb on + strand, within PP_3724 at 4249.528 kb on - strand, within PP_3724 at 4249.528 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4250.083 kb on - strand at 4250.083 kb on - strand at 4250.364 kb on - strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.576 kb on + strand, within PP_3725 at 4250.577 kb on - strand at 4250.684 kb on + strand, within PP_3725 at 4250.684 kb on + strand, within PP_3725 at 4250.684 kb on + strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand at 4250.879 kb on + strand, within PP_3725 at 4250.879 kb on + strand, within PP_3725 at 4250.996 kb on + strand, within PP_3725 at 4250.996 kb on + strand, within PP_3725 at 4250.997 kb on - strand, within PP_3725
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_21; with MOPS remove 4,247,289 + PP_3723 0.61 -1.2 4,247,290 - PP_3723 0.61 +0.8 4,247,369 - PP_3723 0.66 -1.8 4,247,369 - PP_3723 0.66 +1.7 4,247,776 + +0.0 4,247,776 + -0.1 4,247,777 - -0.7 4,247,777 - +0.7 4,247,777 - +0.8 4,247,777 - -1.3 4,247,777 - -0.2 4,247,777 - -0.9 4,247,777 - +0.4 4,247,777 - -0.1 4,247,777 - +1.3 4,247,777 - -3.3 4,247,810 + +0.4 4,247,811 - -3.6 4,247,862 - +1.2 4,247,862 - -3.3 4,247,862 - +0.1 4,247,862 - -0.3 4,247,969 - -0.3 4,248,037 - -0.9 4,248,037 - +1.3 4,248,266 + PP_3724 0.17 -0.9 4,248,267 - PP_3724 0.17 -0.6 4,248,267 - PP_3724 0.17 +0.1 4,248,336 + PP_3724 0.20 -1.3 4,248,336 + PP_3724 0.20 +0.8 4,248,337 - PP_3724 0.20 -0.5 4,248,337 - PP_3724 0.20 +0.4 4,248,459 - PP_3724 0.26 -1.5 4,248,509 - PP_3724 0.28 -2.1 4,248,667 - PP_3724 0.36 -3.0 4,248,826 - PP_3724 0.43 -1.1 4,249,003 - PP_3724 0.52 -0.1 4,249,237 - PP_3724 0.63 +0.7 4,249,488 + PP_3724 0.75 -2.2 4,249,488 + PP_3724 0.75 -1.3 4,249,528 - PP_3724 0.76 +0.7 4,249,528 - PP_3724 0.76 +0.1 4,249,735 - PP_3724 0.86 +1.3 4,249,735 - PP_3724 0.86 -0.1 4,249,735 - PP_3724 0.86 -1.4 4,249,735 - PP_3724 0.86 -2.6 4,250,083 - +0.8 4,250,083 - -0.0 4,250,364 - PP_3725 0.28 +0.5 4,250,567 + PP_3725 0.45 -1.9 4,250,567 + PP_3725 0.45 -0.1 4,250,567 + PP_3725 0.45 -0.7 4,250,576 + PP_3725 0.46 -1.4 4,250,577 - -0.7 4,250,684 + PP_3725 0.55 +0.6 4,250,684 + PP_3725 0.55 -1.7 4,250,684 + PP_3725 0.55 +0.5 4,250,685 - PP_3725 0.55 -0.8 4,250,685 - PP_3725 0.55 -3.7 4,250,685 - PP_3725 0.55 -1.3 4,250,685 - PP_3725 0.55 +0.1 4,250,685 - +1.3 4,250,879 + PP_3725 0.72 +1.6 4,250,879 + PP_3725 0.72 -0.3 4,250,996 + PP_3725 0.82 +0.2 4,250,996 + PP_3725 0.82 +0.1 4,250,997 - PP_3725 0.82 -0.3
Or see this region's nucleotide sequence