Experiment: Temperature shift 30_to_34; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2454 and PP_2455 overlap by 1 nucleotides PP_2455 and PP_2456 overlap by 4 nucleotides PP_2456 and PP_2457 are separated by 4 nucleotides
PP_2454: PP_2454 - ribose ABC transporter, periplasmic ribose-binding subunit, at 2,800,658 to 2,801,617
_2454
PP_2455: PP_2455 - ribose ABC transporter - ATP-binding subunit, at 2,801,617 to 2,803,191
_2455
PP_2456: PP_2456 - D-ribose ABC transporter - permease subunit, at 2,803,188 to 2,804,183
_2456
PP_2457: PP_2457 - DNA-binding transcriptional repressor, at 2,804,188 to 2,805,210
_2457
Position (kb)
2801
2802
2803
2804 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2800.622 kb on - strand at 2801.052 kb on + strand, within PP_2454 at 2801.123 kb on + strand, within PP_2454 at 2801.123 kb on + strand, within PP_2454 at 2801.167 kb on + strand, within PP_2454 at 2801.168 kb on - strand, within PP_2454 at 2801.322 kb on - strand, within PP_2454 at 2801.513 kb on + strand, within PP_2454 at 2801.513 kb on + strand, within PP_2454 at 2801.514 kb on - strand, within PP_2454 at 2801.514 kb on - strand, within PP_2454 at 2801.514 kb on - strand, within PP_2454 at 2801.692 kb on + strand at 2801.692 kb on + strand at 2801.693 kb on - strand at 2801.782 kb on - strand, within PP_2455 at 2801.782 kb on - strand, within PP_2455 at 2801.796 kb on + strand, within PP_2455 at 2802.235 kb on + strand, within PP_2455 at 2802.236 kb on - strand, within PP_2455 at 2802.236 kb on - strand, within PP_2455 at 2802.236 kb on - strand, within PP_2455 at 2802.237 kb on + strand, within PP_2455 at 2802.238 kb on - strand, within PP_2455 at 2802.332 kb on - strand, within PP_2455 at 2802.332 kb on - strand at 2802.384 kb on + strand, within PP_2455 at 2802.384 kb on + strand, within PP_2455 at 2802.385 kb on - strand, within PP_2455 at 2802.547 kb on + strand, within PP_2455 at 2802.548 kb on - strand, within PP_2455 at 2802.548 kb on - strand, within PP_2455 at 2802.958 kb on + strand, within PP_2455 at 2802.958 kb on + strand, within PP_2455 at 2802.959 kb on - strand, within PP_2455 at 2802.959 kb on - strand, within PP_2455 at 2803.246 kb on - strand at 2803.263 kb on + strand at 2803.264 kb on - strand at 2803.264 kb on - strand at 2803.335 kb on + strand, within PP_2456 at 2803.336 kb on - strand, within PP_2456 at 2803.636 kb on - strand, within PP_2456 at 2803.662 kb on + strand, within PP_2456 at 2803.662 kb on + strand, within PP_2456 at 2803.662 kb on + strand, within PP_2456 at 2803.663 kb on - strand, within PP_2456 at 2803.663 kb on - strand, within PP_2456 at 2803.762 kb on + strand, within PP_2456 at 2803.851 kb on + strand, within PP_2456 at 2803.852 kb on - strand, within PP_2456 at 2804.152 kb on - strand at 2804.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_34; with MOPS remove 2,800,622 - -0.1 2,801,052 + PP_2454 0.41 -0.4 2,801,123 + PP_2454 0.48 -0.0 2,801,123 + PP_2454 0.48 +0.9 2,801,167 + PP_2454 0.53 -0.9 2,801,168 - PP_2454 0.53 -0.2 2,801,322 - PP_2454 0.69 -0.3 2,801,513 + PP_2454 0.89 +0.8 2,801,513 + PP_2454 0.89 +0.9 2,801,514 - PP_2454 0.89 +0.2 2,801,514 - PP_2454 0.89 +0.0 2,801,514 - PP_2454 0.89 -0.2 2,801,692 + -0.5 2,801,692 + +1.9 2,801,693 - +0.7 2,801,782 - PP_2455 0.10 -0.9 2,801,782 - PP_2455 0.10 +0.1 2,801,796 + PP_2455 0.11 -1.9 2,802,235 + PP_2455 0.39 -1.1 2,802,236 - PP_2455 0.39 -1.6 2,802,236 - PP_2455 0.39 +1.1 2,802,236 - PP_2455 0.39 -0.7 2,802,237 + PP_2455 0.39 -0.8 2,802,238 - PP_2455 0.39 -0.9 2,802,332 - PP_2455 0.45 -0.5 2,802,332 - +0.2 2,802,384 + PP_2455 0.49 -0.5 2,802,384 + PP_2455 0.49 -3.1 2,802,385 - PP_2455 0.49 -0.6 2,802,547 + PP_2455 0.59 -0.3 2,802,548 - PP_2455 0.59 -0.7 2,802,548 - PP_2455 0.59 -2.9 2,802,958 + PP_2455 0.85 -3.6 2,802,958 + PP_2455 0.85 -3.3 2,802,959 - PP_2455 0.85 +1.0 2,802,959 - PP_2455 0.85 -1.1 2,803,246 - -0.8 2,803,263 + -0.7 2,803,264 - +0.2 2,803,264 - +0.7 2,803,335 + PP_2456 0.15 -1.0 2,803,336 - PP_2456 0.15 -0.0 2,803,636 - PP_2456 0.45 +0.2 2,803,662 + PP_2456 0.48 -0.3 2,803,662 + PP_2456 0.48 +0.7 2,803,662 + PP_2456 0.48 +0.4 2,803,663 - PP_2456 0.48 +0.9 2,803,663 - PP_2456 0.48 -0.0 2,803,762 + PP_2456 0.58 +0.2 2,803,851 + PP_2456 0.67 -0.7 2,803,852 - PP_2456 0.67 +0.2 2,804,152 - +0.7 2,804,156 + +1.7
Or see this region's nucleotide sequence