Experiment: Temperature shift 10_to_34
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1677 and PP_1678 are separated by 1 nucleotides PP_1678 and PP_1679 overlap by 4 nucleotides PP_1679 and PP_1680 overlap by 4 nucleotides PP_1680 and PP_1681 overlap by 32 nucleotides
PP_1677: PP_1677 - Adenosyl-cobyric acid synthase, at 1,871,716 to 1,873,170
_1677
PP_1678: PP_1678 - bifunctional cobinamide-P guanylyltransferase - adenosylcobinamide kinase, at 1,873,172 to 1,873,693
_1678
PP_1679: PP_1679 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 1,873,690 to 1,874,745
_1679
PP_1680: PP_1680 - alpha-ribazole-5'-phosphate phosphatase, at 1,874,742 to 1,875,308
_1680
PP_1681: PP_1681 - cobalamin 5'-phosphate synthase / Cobalamin synthase, at 1,875,277 to 1,876,032
_1681
Position (kb)
1873
1874
1875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1872.696 kb on + strand, within PP_1677 at 1872.793 kb on + strand, within PP_1677 at 1872.794 kb on - strand, within PP_1677 at 1872.875 kb on - strand, within PP_1677 at 1872.988 kb on + strand, within PP_1677 at 1872.988 kb on + strand, within PP_1677 at 1872.988 kb on + strand, within PP_1677 at 1872.989 kb on - strand, within PP_1677 at 1873.073 kb on - strand at 1873.073 kb on - strand at 1873.178 kb on - strand at 1873.259 kb on + strand, within PP_1678 at 1873.259 kb on + strand, within PP_1678 at 1873.586 kb on + strand, within PP_1678 at 1873.586 kb on + strand, within PP_1678 at 1873.586 kb on + strand, within PP_1678 at 1873.587 kb on - strand, within PP_1678 at 1873.668 kb on + strand at 1873.668 kb on + strand at 1873.669 kb on - strand at 1873.669 kb on - strand at 1873.689 kb on + strand at 1873.689 kb on + strand at 1873.689 kb on + strand at 1873.689 kb on + strand at 1873.689 kb on + strand at 1873.689 kb on + strand at 1873.690 kb on - strand at 1873.690 kb on - strand at 1874.437 kb on + strand, within PP_1679 at 1874.484 kb on + strand, within PP_1679 at 1874.485 kb on - strand, within PP_1679 at 1874.485 kb on - strand, within PP_1679 at 1875.193 kb on + strand, within PP_1680 at 1875.406 kb on + strand, within PP_1681 at 1875.406 kb on + strand, within PP_1681 at 1875.407 kb on - strand, within PP_1681 at 1875.407 kb on - strand, within PP_1681 at 1875.407 kb on - strand, within PP_1681 at 1875.464 kb on + strand, within PP_1681 at 1875.464 kb on + strand, within PP_1681 at 1875.472 kb on - strand, within PP_1681 at 1875.515 kb on + strand, within PP_1681 at 1875.516 kb on - strand, within PP_1681 at 1875.572 kb on + strand, within PP_1681 at 1875.572 kb on + strand, within PP_1681
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_34 remove 1,872,696 + PP_1677 0.67 -3.6 1,872,793 + PP_1677 0.74 -0.2 1,872,794 - PP_1677 0.74 -1.4 1,872,875 - PP_1677 0.80 -0.4 1,872,988 + PP_1677 0.87 -4.2 1,872,988 + PP_1677 0.87 -0.9 1,872,988 + PP_1677 0.87 -0.3 1,872,989 - PP_1677 0.87 -3.6 1,873,073 - -3.0 1,873,073 - -2.1 1,873,178 - -1.6 1,873,259 + PP_1678 0.17 -1.4 1,873,259 + PP_1678 0.17 -2.6 1,873,586 + PP_1678 0.79 -1.0 1,873,586 + PP_1678 0.79 -1.5 1,873,586 + PP_1678 0.79 -1.1 1,873,587 - PP_1678 0.80 -2.5 1,873,668 + -2.1 1,873,668 + -1.2 1,873,669 - -0.6 1,873,669 - -1.8 1,873,689 + +0.0 1,873,689 + -0.0 1,873,689 + +0.2 1,873,689 + +1.3 1,873,689 + -1.0 1,873,689 + +0.0 1,873,690 - -3.9 1,873,690 - -0.9 1,874,437 + PP_1679 0.71 -1.9 1,874,484 + PP_1679 0.75 -1.9 1,874,485 - PP_1679 0.75 -2.9 1,874,485 - PP_1679 0.75 -3.4 1,875,193 + PP_1680 0.80 -1.1 1,875,406 + PP_1681 0.17 -2.5 1,875,406 + PP_1681 0.17 -1.3 1,875,407 - PP_1681 0.17 -1.1 1,875,407 - PP_1681 0.17 -1.3 1,875,407 - PP_1681 0.17 -1.3 1,875,464 + PP_1681 0.25 -0.9 1,875,464 + PP_1681 0.25 -2.1 1,875,472 - PP_1681 0.26 -3.0 1,875,515 + PP_1681 0.31 -2.6 1,875,516 - PP_1681 0.32 -3.0 1,875,572 + PP_1681 0.39 -1.8 1,875,572 + PP_1681 0.39 -1.6
Or see this region's nucleotide sequence