Strain Fitness in Pseudomonas putida KT2440 around PP_4016

Experiment: Temperature shift 10_to_30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4014 and PP_4015 overlap by 4 nucleotidesPP_4015 and PP_4016 are separated by 137 nucleotidesPP_4016 and PP_4017 are separated by 75 nucleotides PP_4014: PP_4014 - tRNA-specific 2-thiouridylase MnmA, at 4,525,070 to 4,526,194 _4014 PP_4015: PP_4015 - HflD-like high frequency lysogenization protein, at 4,526,191 to 4,526,817 _4015 PP_4016: PP_4016 - 5'-phosphoribosyl-4-(N-succinocarboxamide)-5- aminoimidazole lyase (adenylosuccinate lyase), at 4,526,955 to 4,528,325 _4016 PP_4017: PP_4017 - conserved protein of unknown function, at 4,528,401 to 4,529,573 _4017 Position (kb) 4526 4527 4528 4529Strain fitness (log2 ratio) -3 -2 -1 0 1at 4526.596 kb on + strand, within PP_4015at 4526.596 kb on + strand, within PP_4015at 4526.596 kb on + strand, within PP_4015at 4526.596 kb on + strandat 4526.597 kb on - strand, within PP_4015at 4526.597 kb on - strand, within PP_4015at 4526.597 kb on - strand, within PP_4015at 4526.597 kb on - strand, within PP_4015at 4526.597 kb on - strand, within PP_4015at 4526.910 kb on + strandat 4526.910 kb on + strandat 4526.910 kb on + strandat 4526.915 kb on + strandat 4528.323 kb on + strandat 4528.323 kb on + strandat 4528.339 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Temperature shift 10_to_30
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4,526,596 + PP_4015 0.65 -0.3
4,526,596 + PP_4015 0.65 -1.2
4,526,596 + PP_4015 0.65 -0.8
4,526,596 + -0.6
4,526,597 - PP_4015 0.65 +0.6
4,526,597 - PP_4015 0.65 -0.7
4,526,597 - PP_4015 0.65 -3.7
4,526,597 - PP_4015 0.65 +0.3
4,526,597 - PP_4015 0.65 -0.9
4,526,910 + -0.6
4,526,910 + -1.1
4,526,910 + -0.7
4,526,915 + +1.2
4,528,323 + -0.4
4,528,323 + +0.4
4,528,339 + +1.5

Or see this region's nucleotide sequence