Experiment: Temperature shift 10_to_21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0155 and PP_5747 are separated by 2 nucleotides PP_5747 and PP_0156 are separated by 11 nucleotides PP_0156 and PP_0157 are separated by 419 nucleotides
PP_0155: PP_0155 - pyridine nucleotide transhydrogenase, beta subunit, at 164,020 to 165,456
_0155
PP_5747: PP_5747 - pyridine nucleotide transhydrogenase subunit alpha, at 165,459 to 165,773
_5747
PP_0156: PP_0156 - pyridine nucleotide transhydrogenase subunit alpha, at 165,785 to 166,906
_0156
PP_0157: PP_0157 - transcriptional activator of gcdH, LysR family, at 167,326 to 168,243
_0157
Position (kb) 165
166
167 Strain fitness (log2 ratio) -2
-1
0
1
2 at 164.799 kb on + strand, within PP_0155 at 164.799 kb on + strand, within PP_0155 at 164.855 kb on + strand, within PP_0155 at 164.855 kb on + strand, within PP_0155 at 164.855 kb on + strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.942 kb on - strand, within PP_0155 at 164.972 kb on + strand, within PP_0155 at 164.972 kb on + strand, within PP_0155 at 165.018 kb on + strand, within PP_0155 at 165.018 kb on + strand, within PP_0155 at 165.018 kb on + strand, within PP_0155 at 165.019 kb on - strand, within PP_0155 at 165.019 kb on - strand, within PP_0155 at 165.019 kb on - strand, within PP_0155 at 165.260 kb on + strand, within PP_0155 at 165.260 kb on + strand, within PP_0155 at 165.297 kb on + strand, within PP_0155 at 165.297 kb on + strand, within PP_0155 at 165.297 kb on + strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.299 kb on + strand, within PP_0155 at 165.299 kb on + strand, within PP_0155 at 165.300 kb on - strand, within PP_0155 at 165.300 kb on - strand, within PP_0155 at 165.428 kb on + strand at 165.428 kb on + strand at 165.429 kb on - strand at 165.460 kb on + strand at 165.461 kb on - strand at 165.461 kb on - strand at 165.703 kb on + strand, within PP_5747 at 165.704 kb on - strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.743 kb on - strand at 165.787 kb on - strand at 165.918 kb on + strand, within PP_0156 at 165.918 kb on + strand, within PP_0156 at 165.919 kb on - strand, within PP_0156 at 166.400 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.580 kb on - strand, within PP_0156 at 166.700 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.940 kb on + strand at 166.941 kb on - strand at 166.941 kb on - strand at 167.076 kb on + strand at 167.076 kb on + strand at 167.185 kb on - strand at 167.191 kb on - strand at 167.208 kb on + strand at 167.225 kb on + strand at 167.239 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.298 kb on + strand at 167.307 kb on - strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.360 kb on + strand at 167.360 kb on + strand at 167.361 kb on - strand at 167.361 kb on - strand at 167.378 kb on - strand at 167.441 kb on + strand, within PP_0157 at 167.441 kb on + strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.458 kb on - strand, within PP_0157 at 167.476 kb on - strand, within PP_0157 at 167.527 kb on + strand, within PP_0157 at 167.528 kb on - strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.585 kb on + strand, within PP_0157 at 167.586 kb on - strand, within PP_0157 at 167.807 kb on + strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_21 remove 164,799 + PP_0155 0.54 -0.3 164,799 + PP_0155 0.54 -0.9 164,855 + PP_0155 0.58 +0.4 164,855 + PP_0155 0.58 +0.4 164,855 + PP_0155 0.58 +0.8 164,856 - PP_0155 0.58 -1.7 164,856 - PP_0155 0.58 -0.5 164,856 - PP_0155 0.58 +0.7 164,856 - PP_0155 0.58 +0.1 164,856 - PP_0155 0.58 -0.4 164,942 - PP_0155 0.64 +0.1 164,972 + PP_0155 0.66 -0.2 164,972 + PP_0155 0.66 -0.9 165,018 + PP_0155 0.69 +0.2 165,018 + PP_0155 0.69 +0.1 165,018 + PP_0155 0.69 +1.4 165,019 - PP_0155 0.70 +1.3 165,019 - PP_0155 0.70 +0.4 165,019 - PP_0155 0.70 +0.3 165,260 + PP_0155 0.86 -1.2 165,260 + PP_0155 0.86 -0.1 165,297 + PP_0155 0.89 +0.4 165,297 + PP_0155 0.89 -0.2 165,297 + PP_0155 0.89 -0.6 165,298 - PP_0155 0.89 -0.7 165,298 - PP_0155 0.89 -0.6 165,298 - PP_0155 0.89 +0.4 165,298 - PP_0155 0.89 +0.3 165,299 + PP_0155 0.89 +0.5 165,299 + PP_0155 0.89 +0.7 165,300 - PP_0155 0.89 +0.1 165,300 - PP_0155 0.89 +0.6 165,428 + -0.0 165,428 + -0.8 165,429 - -0.3 165,460 + -0.3 165,461 - -1.5 165,461 - +0.4 165,703 + PP_5747 0.77 +0.5 165,704 - PP_5747 0.78 -0.9 165,712 + PP_5747 0.80 +0.8 165,712 + PP_5747 0.80 -0.1 165,712 + PP_5747 0.80 +0.2 165,712 + PP_5747 0.80 -1.6 165,712 + PP_5747 0.80 +0.3 165,712 + PP_5747 0.80 +0.8 165,712 + PP_5747 0.80 +0.3 165,713 - PP_5747 0.81 +0.9 165,713 - PP_5747 0.81 -0.1 165,713 - PP_5747 0.81 +0.2 165,713 - PP_5747 0.81 -0.0 165,713 - PP_5747 0.81 +0.9 165,743 - +0.3 165,787 - +0.6 165,918 + PP_0156 0.12 +0.4 165,918 + PP_0156 0.12 +0.3 165,919 - PP_0156 0.12 +0.0 166,400 + PP_0156 0.55 -1.2 166,464 + PP_0156 0.61 -0.1 166,464 + PP_0156 0.61 +0.8 166,465 - PP_0156 0.61 +0.7 166,465 - PP_0156 0.61 +0.7 166,465 - PP_0156 0.61 -1.3 166,494 + PP_0156 0.63 -0.1 166,494 + PP_0156 0.63 -0.5 166,495 - PP_0156 0.63 +1.0 166,495 - PP_0156 0.63 +1.6 166,580 - PP_0156 0.71 +0.9 166,700 + PP_0156 0.82 -0.9 166,716 + PP_0156 0.83 +0.7 166,716 + PP_0156 0.83 -0.9 166,910 + -1.2 166,910 + +0.2 166,910 + -0.2 166,910 + -1.0 166,910 + +0.1 166,911 - +0.1 166,911 - -0.4 166,911 - -0.3 166,940 + +0.3 166,941 - +0.4 166,941 - -0.8 167,076 + -0.1 167,076 + +0.0 167,185 - -1.3 167,191 - +0.2 167,208 + +0.1 167,225 + +0.5 167,239 - -0.1 167,285 - +0.0 167,285 - +0.2 167,285 - -0.0 167,286 + -0.8 167,286 + -0.1 167,286 + +0.8 167,286 + -0.3 167,286 + -0.0 167,286 + +1.7 167,286 + -0.4 167,286 + -2.3 167,286 + +0.4 167,287 - -0.6 167,287 - +1.7 167,287 - +0.6 167,290 + +1.3 167,290 + -0.2 167,290 + -0.4 167,290 + -0.5 167,290 + +0.7 167,290 + +0.3 167,290 + -0.3 167,290 + -0.0 167,290 + -0.7 167,290 + -0.2 167,290 + -0.3 167,290 + +0.3 167,290 + +0.4 167,290 + +1.3 167,290 + +0.7 167,290 + +0.3 167,291 - -0.7 167,291 - +0.6 167,291 - +0.5 167,291 - +0.0 167,291 - -0.2 167,291 - +0.2 167,291 - -0.3 167,291 - -0.4 167,291 - +0.4 167,291 - +0.0 167,298 + +0.7 167,307 - -1.9 167,352 + -0.0 167,352 + -0.1 167,352 + -0.2 167,352 + -0.1 167,352 + +0.4 167,353 - -0.4 167,353 - +0.9 167,353 - -1.7 167,353 - +0.2 167,356 + +0.5 167,356 + -2.0 167,356 + +0.8 167,356 + -0.1 167,356 + -0.0 167,356 + +0.7 167,357 - -0.2 167,357 - +0.5 167,357 - -2.0 167,357 - -1.1 167,357 - -0.2 167,357 - +0.3 167,360 + +0.2 167,360 + -0.2 167,361 - +2.4 167,361 - +0.7 167,378 - +0.5 167,441 + PP_0157 0.13 -0.1 167,441 + PP_0157 0.13 +0.1 167,442 - PP_0157 0.13 -0.5 167,442 - PP_0157 0.13 +0.0 167,442 - PP_0157 0.13 +0.5 167,445 - PP_0157 0.13 -0.2 167,445 - PP_0157 0.13 +0.1 167,445 - PP_0157 0.13 +0.5 167,458 - PP_0157 0.14 -0.4 167,476 - PP_0157 0.16 -0.4 167,527 + PP_0157 0.22 +0.5 167,528 - PP_0157 0.22 -0.4 167,578 + PP_0157 0.27 -1.0 167,578 + PP_0157 0.27 +0.5 167,578 + PP_0157 0.27 +0.8 167,579 - PP_0157 0.28 +0.6 167,579 - PP_0157 0.28 -0.6 167,585 + PP_0157 0.28 +0.0 167,586 - PP_0157 0.28 +0.1 167,807 + PP_0157 0.52 +0.2 167,808 - PP_0157 0.53 +0.6 167,808 - PP_0157 0.53 +0.2 167,827 - PP_0157 0.55 +0.2 167,827 - PP_0157 0.55 +0.2
Or see this region's nucleotide sequence