Experiment: Plant=Sorghum_B5_999; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5497 and Atu5498 are separated by 127 nucleotides Atu5498 and Atu5499 overlap by 4 nucleotides Atu5499 and Atu5500 overlap by 4 nucleotides
Atu5497: Atu5497 - hypothetical protein, at 487,132 to 488,121
Atu5497
Atu5498: Atu5498 - oxidoreductase with iron-sulfur subunit, at 488,249 to 488,782
Atu5498
Atu5499: Atu5499 - oxidoreductase, at 488,779 to 489,768
Atu5499
Atu5500: Atu5500 - dehydrogenase, at 489,765 to 492,068
Atu5500
Position (kb) 488
489 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 487.263 kb on - strand, within Atu5497 at 487.283 kb on + strand, within Atu5497 at 487.283 kb on + strand, within Atu5497 at 487.349 kb on + strand, within Atu5497 at 487.353 kb on + strand, within Atu5497 at 487.353 kb on + strand, within Atu5497 at 487.361 kb on - strand, within Atu5497 at 487.523 kb on + strand, within Atu5497 at 487.531 kb on - strand, within Atu5497 at 487.560 kb on - strand, within Atu5497 at 487.571 kb on - strand, within Atu5497 at 487.601 kb on + strand, within Atu5497 at 487.601 kb on + strand, within Atu5497 at 487.625 kb on + strand, within Atu5497 at 487.654 kb on - strand, within Atu5497 at 487.708 kb on - strand, within Atu5497 at 487.812 kb on - strand, within Atu5497 at 487.938 kb on - strand, within Atu5497 at 487.940 kb on + strand, within Atu5497 at 487.948 kb on - strand, within Atu5497 at 487.959 kb on - strand, within Atu5497 at 488.040 kb on + strand at 488.040 kb on + strand at 488.040 kb on + strand at 488.048 kb on - strand at 488.048 kb on - strand at 488.048 kb on - strand at 488.055 kb on + strand at 488.095 kb on + strand at 488.140 kb on - strand at 488.162 kb on - strand at 488.212 kb on - strand at 488.244 kb on + strand at 488.252 kb on - strand at 488.308 kb on + strand, within Atu5498 at 488.316 kb on - strand, within Atu5498 at 488.356 kb on - strand, within Atu5498 at 488.368 kb on + strand, within Atu5498 at 488.395 kb on - strand, within Atu5498 at 488.430 kb on - strand, within Atu5498 at 488.440 kb on - strand, within Atu5498 at 488.462 kb on + strand, within Atu5498 at 488.470 kb on - strand, within Atu5498 at 488.470 kb on - strand, within Atu5498 at 488.496 kb on - strand, within Atu5498 at 488.496 kb on - strand, within Atu5498 at 488.557 kb on + strand, within Atu5498 at 488.557 kb on + strand, within Atu5498 at 488.624 kb on - strand, within Atu5498 at 488.714 kb on + strand, within Atu5498 at 488.717 kb on - strand, within Atu5498 at 488.733 kb on + strand at 488.748 kb on + strand at 488.777 kb on - strand at 488.862 kb on + strand at 488.900 kb on + strand, within Atu5499 at 488.908 kb on - strand, within Atu5499 at 488.935 kb on + strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.977 kb on - strand, within Atu5499 at 488.984 kb on + strand, within Atu5499 at 488.989 kb on + strand, within Atu5499 at 488.997 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.030 kb on + strand, within Atu5499 at 489.056 kb on - strand, within Atu5499 at 489.060 kb on + strand, within Atu5499 at 489.243 kb on - strand, within Atu5499 at 489.260 kb on + strand, within Atu5499 at 489.291 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.489 kb on - strand, within Atu5499 at 489.525 kb on + strand, within Atu5499 at 489.527 kb on - strand, within Atu5499 at 489.540 kb on + strand, within Atu5499 at 489.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Sorghum_B5_999; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct remove 487,263 - Atu5497 0.13 -0.7 487,283 + Atu5497 0.15 -0.7 487,283 + Atu5497 0.15 +0.5 487,349 + Atu5497 0.22 -1.1 487,353 + Atu5497 0.22 -2.2 487,353 + Atu5497 0.22 -0.9 487,361 - Atu5497 0.23 +1.3 487,523 + Atu5497 0.39 +0.9 487,531 - Atu5497 0.40 -1.0 487,560 - Atu5497 0.43 -2.9 487,571 - Atu5497 0.44 -1.8 487,601 + Atu5497 0.47 +0.3 487,601 + Atu5497 0.47 +1.9 487,625 + Atu5497 0.50 -2.1 487,654 - Atu5497 0.53 -0.5 487,708 - Atu5497 0.58 -2.7 487,812 - Atu5497 0.69 +0.3 487,938 - Atu5497 0.81 -0.2 487,940 + Atu5497 0.82 -1.1 487,948 - Atu5497 0.82 +1.6 487,959 - Atu5497 0.84 +0.5 488,040 + -2.8 488,040 + -2.2 488,040 + +1.2 488,048 - +0.4 488,048 - +0.7 488,048 - +0.8 488,055 + +0.2 488,095 + +0.2 488,140 - +0.3 488,162 - +0.1 488,212 - +0.7 488,244 + -1.2 488,252 - +0.4 488,308 + Atu5498 0.11 -0.3 488,316 - Atu5498 0.13 -1.5 488,356 - Atu5498 0.20 +2.1 488,368 + Atu5498 0.22 +0.1 488,395 - Atu5498 0.27 +0.5 488,430 - Atu5498 0.34 +0.9 488,440 - Atu5498 0.36 +0.3 488,462 + Atu5498 0.40 -0.2 488,470 - Atu5498 0.41 -0.3 488,470 - Atu5498 0.41 -0.4 488,496 - Atu5498 0.46 -0.9 488,496 - Atu5498 0.46 +0.0 488,557 + Atu5498 0.58 -1.2 488,557 + Atu5498 0.58 -3.4 488,624 - Atu5498 0.70 -0.5 488,714 + Atu5498 0.87 -2.3 488,717 - Atu5498 0.88 -1.3 488,733 + -1.5 488,748 + -1.1 488,777 - -0.1 488,862 + +1.6 488,900 + Atu5499 0.12 -1.1 488,908 - Atu5499 0.13 -0.4 488,935 + Atu5499 0.16 +1.0 488,943 - Atu5499 0.17 +0.9 488,943 - Atu5499 0.17 -0.5 488,977 - Atu5499 0.20 -0.3 488,984 + Atu5499 0.21 +0.7 488,989 + Atu5499 0.21 -0.1 488,997 - Atu5499 0.22 -0.1 489,002 - Atu5499 0.23 -0.1 489,002 - Atu5499 0.23 -0.5 489,030 + Atu5499 0.25 -0.0 489,056 - Atu5499 0.28 +1.1 489,060 + Atu5499 0.28 +0.9 489,243 - Atu5499 0.47 -3.2 489,260 + Atu5499 0.49 +0.8 489,291 + Atu5499 0.52 -0.9 489,481 + Atu5499 0.71 -0.2 489,481 + Atu5499 0.71 -2.2 489,489 - Atu5499 0.72 -2.5 489,525 + Atu5499 0.75 +0.4 489,527 - Atu5499 0.76 +0.5 489,540 + Atu5499 0.77 +0.5 489,736 + -1.5
Or see this region's nucleotide sequence