Experiment: Plant=Sorghum_F72_F505; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5498 and Atu5499 overlap by 4 nucleotides Atu5499 and Atu5500 overlap by 4 nucleotides Atu5500 and Atu5501 are separated by 343 nucleotides
Atu5498: Atu5498 - oxidoreductase with iron-sulfur subunit, at 488,249 to 488,782
Atu5498
Atu5499: Atu5499 - oxidoreductase, at 488,779 to 489,768
Atu5499
Atu5500: Atu5500 - dehydrogenase, at 489,765 to 492,068
Atu5500
Atu5501: Atu5501 - hypothetical protein, at 492,412 to 493,074
Atu5501
Position (kb) 489
490
491
492
493 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 488.777 kb on - strand at 488.862 kb on + strand at 488.900 kb on + strand, within Atu5499 at 488.908 kb on - strand, within Atu5499 at 488.935 kb on + strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.977 kb on - strand, within Atu5499 at 488.984 kb on + strand, within Atu5499 at 488.989 kb on + strand, within Atu5499 at 488.997 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.030 kb on + strand, within Atu5499 at 489.056 kb on - strand, within Atu5499 at 489.060 kb on + strand, within Atu5499 at 489.243 kb on - strand, within Atu5499 at 489.260 kb on + strand, within Atu5499 at 489.291 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.489 kb on - strand, within Atu5499 at 489.525 kb on + strand, within Atu5499 at 489.527 kb on - strand, within Atu5499 at 489.540 kb on + strand, within Atu5499 at 489.736 kb on + strand at 489.833 kb on - strand at 489.833 kb on - strand at 489.869 kb on + strand at 489.870 kb on + strand at 489.871 kb on + strand at 489.878 kb on - strand at 489.878 kb on - strand at 489.878 kb on - strand at 489.879 kb on - strand at 489.949 kb on + strand at 490.030 kb on + strand, within Atu5500 at 490.044 kb on - strand, within Atu5500 at 490.102 kb on - strand, within Atu5500 at 490.189 kb on + strand, within Atu5500 at 490.269 kb on + strand, within Atu5500 at 490.361 kb on + strand, within Atu5500 at 490.369 kb on - strand, within Atu5500 at 490.376 kb on + strand, within Atu5500 at 490.381 kb on + strand, within Atu5500 at 490.389 kb on - strand, within Atu5500 at 490.408 kb on + strand, within Atu5500 at 490.416 kb on - strand, within Atu5500 at 490.437 kb on - strand, within Atu5500 at 490.450 kb on - strand, within Atu5500 at 490.533 kb on + strand, within Atu5500 at 490.539 kb on - strand, within Atu5500 at 490.591 kb on + strand, within Atu5500 at 490.596 kb on + strand, within Atu5500 at 490.608 kb on + strand, within Atu5500 at 490.662 kb on + strand, within Atu5500 at 490.670 kb on - strand, within Atu5500 at 490.687 kb on + strand, within Atu5500 at 490.722 kb on - strand, within Atu5500 at 490.729 kb on - strand, within Atu5500 at 490.745 kb on + strand, within Atu5500 at 490.765 kb on + strand, within Atu5500 at 490.775 kb on + strand, within Atu5500 at 490.817 kb on + strand, within Atu5500 at 490.937 kb on + strand, within Atu5500 at 490.988 kb on + strand, within Atu5500 at 491.077 kb on - strand, within Atu5500 at 491.090 kb on + strand, within Atu5500 at 491.192 kb on + strand, within Atu5500 at 491.200 kb on - strand, within Atu5500 at 491.218 kb on + strand, within Atu5500 at 491.225 kb on + strand, within Atu5500 at 491.243 kb on - strand, within Atu5500 at 491.243 kb on - strand, within Atu5500 at 491.412 kb on - strand, within Atu5500 at 491.477 kb on + strand, within Atu5500 at 491.513 kb on - strand, within Atu5500 at 491.621 kb on - strand, within Atu5500 at 491.641 kb on - strand, within Atu5500 at 491.643 kb on + strand, within Atu5500 at 491.658 kb on + strand, within Atu5500 at 491.666 kb on - strand, within Atu5500 at 491.677 kb on + strand, within Atu5500 at 491.699 kb on + strand, within Atu5500 at 491.756 kb on + strand, within Atu5500 at 491.756 kb on + strand, within Atu5500 at 491.764 kb on - strand, within Atu5500 at 491.764 kb on - strand, within Atu5500 at 491.766 kb on + strand, within Atu5500 at 491.785 kb on + strand, within Atu5500 at 491.843 kb on - strand at 491.868 kb on + strand at 491.933 kb on - strand at 491.935 kb on - strand at 491.942 kb on - strand at 492.044 kb on - strand at 492.062 kb on + strand at 492.066 kb on - strand at 492.088 kb on + strand at 492.123 kb on - strand at 492.181 kb on - strand at 492.227 kb on - strand at 492.232 kb on - strand at 492.252 kb on + strand at 492.276 kb on - strand at 492.324 kb on + strand at 492.332 kb on - strand at 492.410 kb on + strand at 492.454 kb on - strand at 492.637 kb on + strand, within Atu5501 at 492.645 kb on - strand, within Atu5501 at 492.664 kb on - strand, within Atu5501 at 492.833 kb on + strand, within Atu5501 at 492.992 kb on + strand, within Atu5501 at 493.042 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Sorghum_F72_F505; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct remove 488,777 - +0.3 488,862 + +0.8 488,900 + Atu5499 0.12 -1.1 488,908 - Atu5499 0.13 +0.1 488,935 + Atu5499 0.16 -1.8 488,943 - Atu5499 0.17 +0.3 488,943 - Atu5499 0.17 -0.9 488,977 - Atu5499 0.20 +0.2 488,984 + Atu5499 0.21 -2.2 488,989 + Atu5499 0.21 +1.4 488,997 - Atu5499 0.22 +0.3 489,002 - Atu5499 0.23 +1.5 489,002 - Atu5499 0.23 +0.8 489,030 + Atu5499 0.25 -3.9 489,056 - Atu5499 0.28 +1.2 489,060 + Atu5499 0.28 -0.1 489,243 - Atu5499 0.47 +0.1 489,260 + Atu5499 0.49 -0.2 489,291 + Atu5499 0.52 -0.9 489,481 + Atu5499 0.71 -0.6 489,481 + Atu5499 0.71 -0.6 489,489 - Atu5499 0.72 -1.3 489,525 + Atu5499 0.75 +0.2 489,527 - Atu5499 0.76 -1.8 489,540 + Atu5499 0.77 +1.0 489,736 + +0.1 489,833 - +0.4 489,833 - +0.1 489,869 + +1.1 489,870 + -0.3 489,871 + -0.2 489,878 - -1.7 489,878 - -0.4 489,878 - +2.1 489,879 - -0.0 489,949 + +0.2 490,030 + Atu5500 0.12 +0.3 490,044 - Atu5500 0.12 +1.8 490,102 - Atu5500 0.15 +0.2 490,189 + Atu5500 0.18 -0.7 490,269 + Atu5500 0.22 -4.1 490,361 + Atu5500 0.26 +0.1 490,369 - Atu5500 0.26 -1.7 490,376 + Atu5500 0.27 +1.9 490,381 + Atu5500 0.27 -0.4 490,389 - Atu5500 0.27 +0.3 490,408 + Atu5500 0.28 +0.3 490,416 - Atu5500 0.28 +0.0 490,437 - Atu5500 0.29 +0.1 490,450 - Atu5500 0.30 +1.1 490,533 + Atu5500 0.33 +0.3 490,539 - Atu5500 0.34 -0.0 490,591 + Atu5500 0.36 +1.0 490,596 + Atu5500 0.36 -0.4 490,608 + Atu5500 0.37 +0.1 490,662 + Atu5500 0.39 -1.8 490,670 - Atu5500 0.39 -1.2 490,687 + Atu5500 0.40 -1.2 490,722 - Atu5500 0.42 -0.0 490,729 - Atu5500 0.42 +0.5 490,745 + Atu5500 0.43 -0.2 490,765 + Atu5500 0.43 +0.6 490,775 + Atu5500 0.44 -0.6 490,817 + Atu5500 0.46 +0.7 490,937 + Atu5500 0.51 +0.4 490,988 + Atu5500 0.53 -0.1 491,077 - Atu5500 0.57 +0.7 491,090 + Atu5500 0.58 -1.2 491,192 + Atu5500 0.62 -0.1 491,200 - Atu5500 0.62 -0.1 491,218 + Atu5500 0.63 +0.9 491,225 + Atu5500 0.63 -0.7 491,243 - Atu5500 0.64 +1.1 491,243 - Atu5500 0.64 -0.2 491,412 - Atu5500 0.71 -2.0 491,477 + Atu5500 0.74 +0.2 491,513 - Atu5500 0.76 +0.7 491,621 - Atu5500 0.81 +0.4 491,641 - Atu5500 0.81 -0.9 491,643 + Atu5500 0.82 +1.0 491,658 + Atu5500 0.82 +0.4 491,666 - Atu5500 0.83 -2.0 491,677 + Atu5500 0.83 +0.3 491,699 + Atu5500 0.84 +0.3 491,756 + Atu5500 0.86 -2.4 491,756 + Atu5500 0.86 -0.1 491,764 - Atu5500 0.87 +0.2 491,764 - Atu5500 0.87 -1.2 491,766 + Atu5500 0.87 +0.2 491,785 + Atu5500 0.88 +0.2 491,843 - -0.1 491,868 + -0.7 491,933 - -1.1 491,935 - +0.7 491,942 - -1.2 492,044 - +0.1 492,062 + -0.3 492,066 - +0.4 492,088 + +0.8 492,123 - -0.1 492,181 - -0.7 492,227 - +0.3 492,232 - +0.1 492,252 + +0.7 492,276 - +0.3 492,324 + -0.0 492,332 - -0.7 492,410 + -1.2 492,454 - +0.8 492,637 + Atu5501 0.34 -1.7 492,645 - Atu5501 0.35 -0.7 492,664 - Atu5501 0.38 -1.1 492,833 + Atu5501 0.63 +0.4 492,992 + Atu5501 0.87 +1.0 493,042 + -0.1
Or see this region's nucleotide sequence