Experiment: LB_Sorghum Btx 642
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0066 and Atu0068 are separated by 664 nucleotides Atu0068 and Atu0069 are separated by 13 nucleotides Atu0069 and Atu0070 are separated by 92 nucleotides
Atu0066: Atu0066 - putative kinase, at 69,659 to 70,288
Atu0066
Atu0068: Atu0068 - glutaredoxin protein, at 70,953 to 71,174
Atu0068
Atu0069: Atu0069 - NrdI protein involved in ribonucleotide reduction, at 71,188 to 71,586
Atu0069
Atu0070: Atu0070 - ribonucleoside-diphosphate reductase 2 alpha chain, at 71,679 to 73,760
Atu0070
Position (kb)
71
72 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 70.267 kb on - strand at 70.288 kb on + strand at 70.387 kb on - strand at 70.586 kb on + strand at 70.625 kb on + strand at 70.655 kb on + strand at 70.730 kb on + strand at 70.755 kb on - strand at 70.792 kb on + strand at 71.159 kb on - strand at 71.463 kb on - strand, within Atu0069 at 71.718 kb on + strand at 71.725 kb on - strand at 71.765 kb on + strand at 71.849 kb on - strand at 71.855 kb on - strand at 71.975 kb on + strand, within Atu0070 at 71.983 kb on - strand, within Atu0070 at 72.346 kb on + strand, within Atu0070 at 72.563 kb on + strand, within Atu0070 at 72.563 kb on + strand, within Atu0070 at 72.563 kb on + strand, within Atu0070 at 72.571 kb on - strand, within Atu0070 at 72.571 kb on - strand, within Atu0070
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Sorghum Btx 642 remove 70,267 - -0.4 70,288 + -1.1 70,387 - -0.1 70,586 + -0.5 70,625 + -1.6 70,655 + -1.0 70,730 + -2.7 70,755 - -1.7 70,792 + -1.2 71,159 - -0.4 71,463 - Atu0069 0.69 -1.1 71,718 + -1.4 71,725 - -2.1 71,765 + -3.1 71,849 - -0.6 71,855 - -1.3 71,975 + Atu0070 0.14 -1.5 71,983 - Atu0070 0.15 -0.5 72,346 + Atu0070 0.32 -1.3 72,563 + Atu0070 0.42 -1.9 72,563 + Atu0070 0.42 -1.6 72,563 + Atu0070 0.42 -1.7 72,571 - Atu0070 0.43 -0.6 72,571 - Atu0070 0.43 -1.5
Or see this region's nucleotide sequence