Experiment: LB_Sorghum SS 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0068 and Atu0069 are separated by 13 nucleotides Atu0069 and Atu0070 are separated by 92 nucleotides Atu0070 and Atu0071 are separated by 16 nucleotides
Atu0068: Atu0068 - glutaredoxin protein, at 70,953 to 71,174
Atu0068
Atu0069: Atu0069 - NrdI protein involved in ribonucleotide reduction, at 71,188 to 71,586
Atu0069
Atu0070: Atu0070 - ribonucleoside-diphosphate reductase 2 alpha chain, at 71,679 to 73,760
Atu0070
Atu0071: Atu0071 - ribonucleoside-diphosphate reductase 2 beta chain, at 73,777 to 74,751
Atu0071
Position (kb)
71
72
73
74 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 70.730 kb on + strand at 70.755 kb on - strand at 70.792 kb on + strand at 71.159 kb on - strand at 71.463 kb on - strand, within Atu0069 at 71.718 kb on + strand at 71.725 kb on - strand at 71.765 kb on + strand at 71.849 kb on - strand at 71.855 kb on - strand at 71.975 kb on + strand, within Atu0070 at 71.983 kb on - strand, within Atu0070 at 72.346 kb on + strand, within Atu0070 at 72.563 kb on + strand, within Atu0070 at 72.563 kb on + strand, within Atu0070 at 72.563 kb on + strand, within Atu0070 at 72.571 kb on - strand, within Atu0070 at 72.571 kb on - strand, within Atu0070 at 72.704 kb on + strand, within Atu0070 at 72.982 kb on + strand, within Atu0070 at 73.032 kb on + strand, within Atu0070 at 73.042 kb on + strand, within Atu0070 at 73.050 kb on - strand, within Atu0070 at 73.171 kb on - strand, within Atu0070 at 73.189 kb on + strand, within Atu0070 at 73.361 kb on + strand, within Atu0070 at 73.465 kb on - strand, within Atu0070 at 73.647 kb on - strand at 73.686 kb on + strand at 73.731 kb on + strand at 73.731 kb on + strand at 73.731 kb on - strand at 73.759 kb on + strand at 73.837 kb on - strand at 73.996 kb on + strand, within Atu0071 at 74.087 kb on - strand, within Atu0071 at 74.140 kb on + strand, within Atu0071 at 74.152 kb on + strand, within Atu0071 at 74.301 kb on + strand, within Atu0071 at 74.306 kb on + strand, within Atu0071 at 74.586 kb on + strand, within Atu0071 at 74.760 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Sorghum SS 20 remove 70,730 + -1.6 70,755 - -1.8 70,792 + -1.2 71,159 - -0.1 71,463 - Atu0069 0.69 -1.1 71,718 + -1.7 71,725 - -1.3 71,765 + -3.1 71,849 - -2.1 71,855 - -1.6 71,975 + Atu0070 0.14 -1.6 71,983 - Atu0070 0.15 +0.4 72,346 + Atu0070 0.32 -1.7 72,563 + Atu0070 0.42 -1.2 72,563 + Atu0070 0.42 -1.3 72,563 + Atu0070 0.42 -1.1 72,571 - Atu0070 0.43 -2.2 72,571 - Atu0070 0.43 -1.8 72,704 + Atu0070 0.49 -0.4 72,982 + Atu0070 0.63 -2.0 73,032 + Atu0070 0.65 -0.4 73,042 + Atu0070 0.65 +1.0 73,050 - Atu0070 0.66 -1.3 73,171 - Atu0070 0.72 +0.4 73,189 + Atu0070 0.73 -1.6 73,361 + Atu0070 0.81 -1.1 73,465 - Atu0070 0.86 -1.1 73,647 - -1.3 73,686 + -1.4 73,731 + +0.0 73,731 + -1.4 73,731 - -1.7 73,759 + -0.1 73,837 - +0.0 73,996 + Atu0071 0.22 -1.8 74,087 - Atu0071 0.32 -1.3 74,140 + Atu0071 0.37 -0.8 74,152 + Atu0071 0.38 -1.1 74,301 + Atu0071 0.54 -1.3 74,306 + Atu0071 0.54 -1.0 74,586 + Atu0071 0.83 -2.0 74,760 - +0.5
Or see this region's nucleotide sequence