Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4741 and PP_4742 overlap by 4 nucleotides PP_4742 and PP_5711 are separated by 10 nucleotides PP_5711 and PP_4743 are separated by 29 nucleotides
PP_4741: PP_4741 - Type I restriction enzyme EcoEI M protein, at 5,395,523 to 5,396,992
_4741
PP_4742: PP_4742 - Type I restriction-modification system, S subunit, at 5,396,989 to 5,398,719
_4742
PP_5711: PP_5711 - protein of unknown function, at 5,398,730 to 5,399,158
_5711
PP_4743: PP_4743 - conserved protein of unknown function, at 5,399,188 to 5,400,624
_4743
Position (kb) 5396
5397
5398
5399 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5396.015 kb on + strand, within PP_4741 at 5396.015 kb on + strand, within PP_4741 at 5396.015 kb on + strand, within PP_4741 at 5396.016 kb on - strand, within PP_4741 at 5396.016 kb on - strand, within PP_4741 at 5396.016 kb on - strand, within PP_4741 at 5396.144 kb on + strand, within PP_4741 at 5396.145 kb on - strand, within PP_4741 at 5396.145 kb on - strand, within PP_4741 at 5396.145 kb on - strand, within PP_4741 at 5396.149 kb on - strand, within PP_4741 at 5396.149 kb on - strand, within PP_4741 at 5396.149 kb on - strand, within PP_4741 at 5396.335 kb on + strand, within PP_4741 at 5396.336 kb on - strand, within PP_4741 at 5396.336 kb on - strand, within PP_4741 at 5396.336 kb on - strand, within PP_4741 at 5396.436 kb on + strand, within PP_4741 at 5396.437 kb on - strand, within PP_4741 at 5396.443 kb on + strand, within PP_4741 at 5396.480 kb on + strand, within PP_4741 at 5396.482 kb on - strand, within PP_4741 at 5396.664 kb on - strand, within PP_4741 at 5396.898 kb on + strand at 5396.898 kb on + strand at 5396.898 kb on + strand at 5396.898 kb on + strand at 5396.899 kb on - strand at 5396.909 kb on + strand at 5396.910 kb on - strand at 5396.910 kb on - strand at 5396.910 kb on - strand at 5396.910 kb on - strand at 5396.910 kb on - strand at 5396.940 kb on + strand at 5396.961 kb on + strand at 5396.989 kb on - strand at 5396.999 kb on + strand at 5397.088 kb on - strand at 5397.108 kb on + strand at 5397.209 kb on + strand, within PP_4742 at 5397.240 kb on - strand, within PP_4742 at 5397.240 kb on - strand, within PP_4742 at 5397.329 kb on + strand, within PP_4742 at 5397.387 kb on + strand, within PP_4742 at 5397.387 kb on + strand, within PP_4742 at 5397.582 kb on - strand, within PP_4742 at 5397.656 kb on + strand, within PP_4742 at 5397.740 kb on + strand, within PP_4742 at 5397.749 kb on + strand, within PP_4742 at 5397.749 kb on + strand, within PP_4742 at 5397.981 kb on + strand, within PP_4742 at 5398.145 kb on + strand, within PP_4742 at 5398.145 kb on + strand, within PP_4742 at 5398.146 kb on - strand, within PP_4742 at 5398.192 kb on - strand, within PP_4742 at 5398.256 kb on + strand, within PP_4742 at 5398.277 kb on + strand, within PP_4742 at 5398.277 kb on + strand, within PP_4742 at 5398.278 kb on - strand, within PP_4742 at 5398.326 kb on + strand, within PP_4742 at 5398.328 kb on + strand, within PP_4742 at 5398.329 kb on - strand, within PP_4742 at 5398.332 kb on - strand, within PP_4742 at 5398.400 kb on + strand, within PP_4742 at 5398.498 kb on + strand, within PP_4742 at 5398.499 kb on - strand, within PP_4742 at 5398.542 kb on + strand, within PP_4742 at 5398.650 kb on - strand at 5398.661 kb on + strand at 5398.760 kb on + strand at 5398.816 kb on - strand, within PP_5711 at 5398.872 kb on + strand, within PP_5711 at 5398.873 kb on - strand, within PP_5711 at 5398.886 kb on + strand, within PP_5711 at 5398.933 kb on + strand, within PP_5711 at 5398.966 kb on + strand, within PP_5711 at 5399.156 kb on + strand at 5399.161 kb on - strand at 5399.323 kb on - strand at 5399.410 kb on - strand, within PP_4743
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 5,396,015 + PP_4741 0.33 -0.8 5,396,015 + PP_4741 0.33 -0.8 5,396,015 + PP_4741 0.33 -2.1 5,396,016 - PP_4741 0.34 +1.8 5,396,016 - PP_4741 0.34 +0.5 5,396,016 - PP_4741 0.34 -0.8 5,396,144 + PP_4741 0.42 +1.0 5,396,145 - PP_4741 0.42 -4.2 5,396,145 - PP_4741 0.42 +1.6 5,396,145 - PP_4741 0.42 -3.4 5,396,149 - PP_4741 0.43 +1.1 5,396,149 - PP_4741 0.43 +1.5 5,396,149 - PP_4741 0.43 -0.2 5,396,335 + PP_4741 0.55 +0.1 5,396,336 - PP_4741 0.55 +0.4 5,396,336 - PP_4741 0.55 -2.0 5,396,336 - PP_4741 0.55 -0.2 5,396,436 + PP_4741 0.62 -4.3 5,396,437 - PP_4741 0.62 -0.7 5,396,443 + PP_4741 0.63 -3.5 5,396,480 + PP_4741 0.65 -3.5 5,396,482 - PP_4741 0.65 -0.6 5,396,664 - PP_4741 0.78 -0.3 5,396,898 + -2.1 5,396,898 + -2.0 5,396,898 + -1.1 5,396,898 + -1.1 5,396,899 - +1.1 5,396,909 + -0.3 5,396,910 - +0.3 5,396,910 - +0.5 5,396,910 - -2.7 5,396,910 - +1.4 5,396,910 - +1.6 5,396,940 + +2.2 5,396,961 + +0.2 5,396,989 - -3.2 5,396,999 + -1.5 5,397,088 - +1.3 5,397,108 + -1.9 5,397,209 + PP_4742 0.13 -1.1 5,397,240 - PP_4742 0.15 -3.6 5,397,240 - PP_4742 0.15 -3.9 5,397,329 + PP_4742 0.20 -0.1 5,397,387 + PP_4742 0.23 -3.3 5,397,387 + PP_4742 0.23 -0.0 5,397,582 - PP_4742 0.34 -0.7 5,397,656 + PP_4742 0.39 -0.2 5,397,740 + PP_4742 0.43 +0.9 5,397,749 + PP_4742 0.44 -3.2 5,397,749 + PP_4742 0.44 +0.8 5,397,981 + PP_4742 0.57 +0.6 5,398,145 + PP_4742 0.67 -2.3 5,398,145 + PP_4742 0.67 -0.0 5,398,146 - PP_4742 0.67 -0.5 5,398,192 - PP_4742 0.69 +2.0 5,398,256 + PP_4742 0.73 +1.1 5,398,277 + PP_4742 0.74 -0.3 5,398,277 + PP_4742 0.74 +1.4 5,398,278 - PP_4742 0.74 -0.9 5,398,326 + PP_4742 0.77 -0.1 5,398,328 + PP_4742 0.77 -3.5 5,398,329 - PP_4742 0.77 +0.4 5,398,332 - PP_4742 0.78 +0.8 5,398,400 + PP_4742 0.82 -1.6 5,398,498 + PP_4742 0.87 +0.4 5,398,499 - PP_4742 0.87 -0.4 5,398,542 + PP_4742 0.90 +0.2 5,398,650 - +1.2 5,398,661 + +2.1 5,398,760 + +1.4 5,398,816 - PP_5711 0.20 -1.7 5,398,872 + PP_5711 0.33 -2.4 5,398,873 - PP_5711 0.33 +0.6 5,398,886 + PP_5711 0.36 -0.4 5,398,933 + PP_5711 0.47 -0.6 5,398,966 + PP_5711 0.55 -1.3 5,399,156 + -1.0 5,399,161 - +0.6 5,399,323 - -1.0 5,399,410 - PP_4743 0.15 +2.0
Or see this region's nucleotide sequence