Strain Fitness in Pseudomonas putida KT2440 around PP_1900

Experiment: Growth at pH8 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1898 and PP_1899 overlap by 4 nucleotidesPP_1899 and PP_1900 overlap by 4 nucleotidesPP_1900 and PP_1901 are separated by 44 nucleotidesPP_1901 and PP_1902 overlap by 4 nucleotidesPP_1902 and PP_1903 overlap by 1 nucleotides PP_1898: PP_1898 - MotA/TolQ/ExbB proton channel family protein, at 2,142,550 to 2,143,224 _1898 PP_1899: PP_1899 - ExbD/TolR family protein, at 2,143,221 to 2,143,655 _1899 PP_1900: PP_1900 - Tetraacyldisaccharide 4'-kinase, at 2,143,652 to 2,144,656 _1900 PP_1901: PP_1901 - conserved protein of unknown function, UPF0434 family, at 2,144,701 to 2,144,886 _1901 PP_1902: PP_1902 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,144,883 to 2,145,647 _1902 PP_1903: PP_1903 - putative phosphotyrosine-protein phosphatase, at 2,145,647 to 2,146,111 _1903 Position (kb) 2143 2144 2145Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2142.660 kb on + strand, within PP_1898at 2142.728 kb on + strand, within PP_1898at 2142.728 kb on + strand, within PP_1898at 2142.904 kb on + strand, within PP_1898at 2143.041 kb on + strand, within PP_1898at 2143.086 kb on + strand, within PP_1898at 2143.316 kb on + strand, within PP_1899at 2144.686 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose
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2,142,660 + PP_1898 0.16 -3.5
2,142,728 + PP_1898 0.26 -3.0
2,142,728 + PP_1898 0.26 -3.6
2,142,904 + PP_1898 0.52 -4.6
2,143,041 + PP_1898 0.73 -4.2
2,143,086 + PP_1898 0.79 -2.5
2,143,316 + PP_1899 0.22 +0.8
2,144,686 + +1.1

Or see this region's nucleotide sequence