Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0430 and PP_0431 are separated by 28 nucleotides PP_0431 and PP_0432 are separated by 142 nucleotides PP_0432 and PP_0433 are separated by 99 nucleotides PP_0433 and PP_0434 are separated by 59 nucleotides
PP_0430: PP_0430 - conserved protein of unknown function, at 517,059 to 518,015
_0430
PP_0431: PP_0431 - conserved membrane protein of unknown function, at 518,044 to 518,469
_0431
PP_0432: PP_0432 - N-acetyl-gamma-glutamyl-phosphate reductase 1, at 518,612 to 519,646
_0432
PP_0433: PP_0433 - Iron-sulfur cluster insertion protein ErpA, at 519,746 to 520,144
_0433
PP_0434: PP_0434 - Anhydro-N-acetylmuramic acid kinase, at 520,204 to 521,295
_0434
Position (kb) 518
519
520 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 517.695 kb on + strand, within PP_0430 at 517.695 kb on + strand, within PP_0430 at 517.695 kb on + strand, within PP_0430 at 517.696 kb on - strand, within PP_0430 at 517.696 kb on - strand, within PP_0430 at 517.726 kb on - strand, within PP_0430 at 517.726 kb on - strand, within PP_0430 at 517.726 kb on - strand, within PP_0430 at 517.801 kb on + strand, within PP_0430 at 518.007 kb on + strand at 518.008 kb on - strand at 518.008 kb on - strand at 518.041 kb on - strand at 518.041 kb on - strand at 518.489 kb on - strand at 518.505 kb on + strand at 518.505 kb on + strand at 518.505 kb on + strand at 518.505 kb on + strand at 518.506 kb on - strand at 518.506 kb on - strand at 518.506 kb on - strand at 518.508 kb on + strand at 518.508 kb on + strand at 518.508 kb on + strand at 518.509 kb on - strand at 518.509 kb on - strand at 518.509 kb on - strand at 518.546 kb on + strand at 518.547 kb on - strand at 518.566 kb on - strand at 518.568 kb on + strand at 518.569 kb on - strand at 518.598 kb on - strand at 518.611 kb on + strand at 518.747 kb on + strand, within PP_0432 at 518.747 kb on + strand, within PP_0432 at 518.748 kb on - strand, within PP_0432 at 518.768 kb on + strand, within PP_0432 at 518.768 kb on + strand, within PP_0432 at 518.951 kb on + strand, within PP_0432 at 518.996 kb on + strand, within PP_0432 at 518.997 kb on - strand, within PP_0432 at 518.997 kb on - strand, within PP_0432 at 518.997 kb on - strand, within PP_0432 at 519.367 kb on + strand, within PP_0432 at 519.764 kb on + strand at 520.142 kb on + strand at 520.143 kb on - strand at 520.155 kb on + strand at 520.155 kb on + strand at 520.205 kb on + strand at 520.337 kb on - strand, within PP_0434 at 520.343 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.376 kb on - strand, within PP_0434
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 517,695 + PP_0430 0.66 +0.4 517,695 + PP_0430 0.66 -3.4 517,695 + PP_0430 0.66 +0.9 517,696 - PP_0430 0.67 +1.1 517,696 - PP_0430 0.67 +1.1 517,726 - PP_0430 0.70 +0.9 517,726 - PP_0430 0.70 +0.2 517,726 - PP_0430 0.70 -1.3 517,801 + PP_0430 0.78 -3.1 518,007 + +0.8 518,008 - -1.7 518,008 - +0.4 518,041 - -2.4 518,041 - +0.3 518,489 - -0.9 518,505 + +2.7 518,505 + +2.2 518,505 + -0.6 518,505 + +0.8 518,506 - -0.8 518,506 - -2.3 518,506 - -1.0 518,508 + -0.1 518,508 + -0.4 518,508 + -3.2 518,509 - -0.4 518,509 - -1.8 518,509 - +0.3 518,546 + -1.7 518,547 - -2.2 518,566 - +1.7 518,568 + +0.6 518,569 - +1.2 518,598 - +2.9 518,611 + -0.6 518,747 + PP_0432 0.13 +0.7 518,747 + PP_0432 0.13 +2.0 518,748 - PP_0432 0.13 +0.7 518,768 + PP_0432 0.15 -2.7 518,768 + PP_0432 0.15 +1.4 518,951 + PP_0432 0.33 -3.1 518,996 + PP_0432 0.37 -2.2 518,997 - PP_0432 0.37 +0.8 518,997 - PP_0432 0.37 +0.8 518,997 - PP_0432 0.37 +0.2 519,367 + PP_0432 0.73 +1.2 519,764 + +0.5 520,142 + -3.8 520,143 - +0.2 520,155 + -2.4 520,155 + -0.1 520,205 + +0.2 520,337 - PP_0434 0.12 -3.3 520,343 + PP_0434 0.13 -0.6 520,375 + PP_0434 0.16 -0.7 520,375 + PP_0434 0.16 -3.1 520,376 - PP_0434 0.16 +1.3
Or see this region's nucleotide sequence