Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0002 and PP_0003 are separated by 18 nucleotides PP_0003 and PP_0004 overlap by 4 nucleotides PP_0004 and PP_0005 are separated by 630 nucleotides
PP_0002: PP_0002 - probable chromosome partition protein, at 1,029 to 1,820
_0002
PP_0003: PP_0003 - 16S RNA methyltransferase, at 1,839 to 2,489
_0003
PP_0004: PP_0004 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme, at 2,486 to 4,381
_0004
PP_0005: PP_0005 - GTPase, at 5,012 to 6,382
_0005
Position (kb)
2
3
4
5 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1.594 kb on + strand, within PP_0002 at 1.595 kb on - strand, within PP_0002 at 1.602 kb on - strand, within PP_0002 at 1.639 kb on + strand, within PP_0002 at 1.639 kb on + strand, within PP_0002 at 1.760 kb on + strand at 1.760 kb on + strand at 1.760 kb on + strand at 1.760 kb on + strand at 1.761 kb on - strand at 1.761 kb on - strand at 1.761 kb on - strand at 1.761 kb on - strand at 1.761 kb on - strand at 1.809 kb on + strand at 1.809 kb on + strand at 1.810 kb on - strand at 1.810 kb on - strand at 1.814 kb on + strand at 1.815 kb on - strand at 1.939 kb on - strand, within PP_0003 at 2.258 kb on + strand, within PP_0003 at 2.258 kb on + strand, within PP_0003 at 2.259 kb on - strand, within PP_0003 at 2.291 kb on + strand, within PP_0003 at 2.291 kb on + strand, within PP_0003 at 2.353 kb on + strand, within PP_0003 at 2.353 kb on + strand, within PP_0003 at 2.354 kb on - strand, within PP_0003 at 4.416 kb on - strand at 4.428 kb on - strand at 4.428 kb on - strand at 4.428 kb on - strand at 4.544 kb on + strand at 4.550 kb on + strand at 4.550 kb on + strand at 4.551 kb on - strand at 4.590 kb on + strand at 4.603 kb on + strand at 4.603 kb on + strand at 4.604 kb on - strand at 4.614 kb on + strand at 4.615 kb on - strand at 4.672 kb on + strand at 4.673 kb on - strand at 4.723 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.979 kb on - strand at 5.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 1,594 + PP_0002 0.71 +0.1 1,595 - PP_0002 0.71 -0.1 1,602 - PP_0002 0.72 -1.1 1,639 + PP_0002 0.77 -3.2 1,639 + PP_0002 0.77 -1.0 1,760 + -0.7 1,760 + -2.7 1,760 + +0.9 1,760 + -2.8 1,761 - -2.5 1,761 - -2.0 1,761 - -1.1 1,761 - -0.9 1,761 - -1.1 1,809 + +0.2 1,809 + -1.8 1,810 - -2.3 1,810 - -0.7 1,814 + +0.8 1,815 - -1.7 1,939 - PP_0003 0.15 -2.6 2,258 + PP_0003 0.64 -2.7 2,258 + PP_0003 0.64 +0.1 2,259 - PP_0003 0.65 -0.5 2,291 + PP_0003 0.69 -0.5 2,291 + PP_0003 0.69 -0.0 2,353 + PP_0003 0.79 +0.8 2,353 + PP_0003 0.79 +1.9 2,354 - PP_0003 0.79 -2.4 4,416 - +1.6 4,428 - +0.3 4,428 - -3.2 4,428 - +0.1 4,544 + -1.7 4,550 + -3.7 4,550 + +0.2 4,551 - +1.4 4,590 + -3.4 4,603 + +1.5 4,603 + +0.2 4,604 - +0.5 4,614 + +1.1 4,615 - -0.1 4,672 + +0.6 4,673 - -2.8 4,723 + -3.6 4,853 + +1.3 4,853 + -0.3 4,853 + -0.9 4,853 + -0.6 4,853 + -2.4 4,853 + -1.0 4,853 + -3.6 4,854 - -0.5 4,854 - +0.2 4,854 - -2.7 4,854 - -4.4 4,854 - -0.5 4,854 - +0.6 4,854 - +0.4 4,878 + -1.2 4,878 + -1.4 4,878 + -3.3 4,878 + -1.9 4,878 + +1.7 4,878 + -0.1 4,879 - +0.1 4,879 - -4.0 4,879 - +1.9 4,879 - -2.4 4,879 - -2.8 4,979 - -0.5 5,014 - -3.5
Or see this region's nucleotide sequence