Experiment: Growth at pH7 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0100 and PP_0101 are separated by 207 nucleotides PP_0101 and PP_5426 are separated by 147 nucleotides
PP_0100: PP_0100 - carbonic anhydrase, at 105,207 to 105,926
_0100
PP_0101: PP_0101 - putative sulfate transporter, at 106,134 to 107,651
_0101
PP_5426: PP_5426 - conserved protein of unknown function, at 107,799 to 108,440
_5426
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 105.148 kb on + strand at 105.149 kb on - strand at 105.149 kb on - strand at 105.179 kb on + strand at 105.179 kb on + strand at 105.183 kb on + strand at 105.922 kb on - strand at 105.924 kb on + strand at 105.924 kb on + strand at 105.924 kb on + strand at 105.924 kb on + strand at 105.925 kb on - strand at 105.954 kb on + strand at 106.101 kb on - strand at 106.127 kb on + strand at 106.127 kb on + strand at 106.128 kb on - strand at 106.128 kb on - strand at 106.128 kb on - strand at 106.251 kb on - strand at 106.333 kb on + strand, within PP_0101 at 106.334 kb on - strand, within PP_0101 at 106.470 kb on + strand, within PP_0101 at 106.717 kb on + strand, within PP_0101 at 106.718 kb on - strand, within PP_0101 at 106.743 kb on - strand, within PP_0101 at 106.743 kb on - strand, within PP_0101 at 106.850 kb on + strand, within PP_0101 at 106.851 kb on - strand, within PP_0101 at 106.921 kb on + strand, within PP_0101 at 106.921 kb on + strand, within PP_0101 at 107.030 kb on - strand, within PP_0101 at 107.250 kb on - strand, within PP_0101 at 107.254 kb on - strand, within PP_0101 at 107.276 kb on + strand, within PP_0101 at 107.302 kb on + strand, within PP_0101 at 107.302 kb on + strand, within PP_0101 at 107.303 kb on - strand, within PP_0101 at 107.303 kb on - strand, within PP_0101 at 107.460 kb on - strand, within PP_0101 at 107.508 kb on + strand at 107.508 kb on + strand at 107.509 kb on - strand at 107.509 kb on - strand at 107.509 kb on - strand at 107.509 kb on - strand at 107.510 kb on + strand at 107.511 kb on - strand at 107.511 kb on - strand at 107.511 kb on - strand at 107.555 kb on + strand at 107.555 kb on + strand at 107.650 kb on - strand at 107.672 kb on - strand at 107.749 kb on + strand at 107.749 kb on + strand at 107.749 kb on + strand at 107.781 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.797 kb on + strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.896 kb on + strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 108.067 kb on + strand, within PP_5426 at 108.068 kb on - strand, within PP_5426 at 108.106 kb on + strand, within PP_5426 at 108.107 kb on - strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.172 kb on + strand, within PP_5426 at 108.173 kb on - strand, within PP_5426 at 108.192 kb on + strand, within PP_5426 at 108.274 kb on + strand, within PP_5426 at 108.274 kb on + strand, within PP_5426 at 108.274 kb on + strand, within PP_5426 at 108.275 kb on - strand, within PP_5426 at 108.412 kb on - strand at 108.412 kb on - strand at 108.458 kb on + strand at 108.458 kb on + strand at 108.459 kb on - strand at 108.459 kb on - strand at 108.459 kb on - strand at 108.459 kb on - strand at 108.480 kb on - strand at 108.485 kb on - strand at 108.493 kb on - strand at 108.494 kb on + strand at 108.495 kb on - strand at 108.513 kb on - strand at 108.522 kb on - strand at 108.522 kb on - strand at 108.522 kb on - strand at 108.566 kb on + strand at 108.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH7 and (C) D-Glucose remove 105,148 + -4.0 105,149 - +1.8 105,149 - -0.8 105,179 + +0.9 105,179 + +1.0 105,183 + +0.2 105,922 - -0.4 105,924 + -2.0 105,924 + -2.7 105,924 + +0.5 105,924 + -2.5 105,925 - -0.6 105,954 + +0.7 106,101 - +0.2 106,127 + -0.4 106,127 + -4.0 106,128 - +1.1 106,128 - +0.8 106,128 - +1.0 106,251 - +2.1 106,333 + PP_0101 0.13 -3.3 106,334 - PP_0101 0.13 -4.0 106,470 + PP_0101 0.22 -2.0 106,717 + PP_0101 0.38 +0.5 106,718 - PP_0101 0.38 -3.6 106,743 - PP_0101 0.40 -1.8 106,743 - PP_0101 0.40 -1.2 106,850 + PP_0101 0.47 -3.1 106,851 - PP_0101 0.47 +1.1 106,921 + PP_0101 0.52 -0.5 106,921 + PP_0101 0.52 +1.3 107,030 - PP_0101 0.59 -3.3 107,250 - PP_0101 0.74 -0.4 107,254 - PP_0101 0.74 -2.9 107,276 + PP_0101 0.75 +0.3 107,302 + PP_0101 0.77 -3.7 107,302 + PP_0101 0.77 +0.7 107,303 - PP_0101 0.77 -1.1 107,303 - PP_0101 0.77 +2.4 107,460 - PP_0101 0.87 +0.6 107,508 + +1.0 107,508 + -2.0 107,509 - +3.1 107,509 - +1.5 107,509 - -3.5 107,509 - -2.9 107,510 + +0.0 107,511 - -0.2 107,511 - -4.1 107,511 - +0.2 107,555 + +1.3 107,555 + -0.1 107,650 - +1.6 107,672 - +0.4 107,749 + -0.2 107,749 + -2.6 107,749 + -2.8 107,781 + +0.7 107,795 + +1.3 107,795 + +2.8 107,795 + +1.7 107,795 + -1.2 107,795 + +1.4 107,795 + +1.6 107,795 + -3.5 107,795 + +0.1 107,796 - -2.9 107,796 - +2.0 107,796 - +1.0 107,796 - -3.4 107,796 - +0.8 107,797 + +2.5 107,798 - -0.1 107,798 - +0.0 107,798 - +2.7 107,798 - +1.1 107,798 - +0.7 107,798 - +0.8 107,896 + PP_5426 0.15 +0.6 107,934 - PP_5426 0.21 +1.7 107,934 - PP_5426 0.21 -1.8 107,934 - PP_5426 0.21 -2.3 107,934 - PP_5426 0.21 -3.3 108,067 + PP_5426 0.42 -2.4 108,068 - PP_5426 0.42 +0.3 108,106 + PP_5426 0.48 -0.2 108,107 - PP_5426 0.48 -2.3 108,135 + PP_5426 0.52 +0.9 108,135 + PP_5426 0.52 -3.4 108,135 + PP_5426 0.52 +1.3 108,135 + PP_5426 0.52 -0.9 108,135 + PP_5426 0.52 +0.7 108,136 - PP_5426 0.52 -1.8 108,136 - PP_5426 0.52 -2.2 108,136 - PP_5426 0.52 -2.2 108,136 - PP_5426 0.52 +0.5 108,172 + PP_5426 0.58 -0.2 108,173 - PP_5426 0.58 -1.9 108,192 + PP_5426 0.61 +1.1 108,274 + PP_5426 0.74 +0.5 108,274 + PP_5426 0.74 -3.6 108,274 + PP_5426 0.74 +0.9 108,275 - PP_5426 0.74 +1.5 108,412 - +2.5 108,412 - -3.4 108,458 + +1.1 108,458 + -0.9 108,459 - -3.4 108,459 - +1.0 108,459 - -0.3 108,459 - -2.4 108,480 - +1.2 108,485 - -1.2 108,493 - -1.1 108,494 + -3.0 108,495 - +2.0 108,513 - -0.6 108,522 - +1.3 108,522 - +0.7 108,522 - +1.1 108,566 + -0.2 108,567 - +0.7
Or see this region's nucleotide sequence