Strain Fitness in Pseudomonas putida KT2440 around PP_1023

Experiment: Growth at pH6 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1022 and PP_1023 overlap by 29 nucleotidesPP_1023 and PP_1024 overlap by 10 nucleotidesPP_1024 and PP_1025 are separated by 252 nucleotides PP_1022: PP_1022 - glucose 6-phosphate-1-dehydrogenase, at 1,165,616 to 1,167,085 _1022 PP_1023: PP_1023 - 6-phosphogluconolactonase, at 1,167,057 to 1,167,785 _1023 PP_1024: PP_1024 - KHG/KDPG aldolase, at 1,167,776 to 1,168,483 _1024 PP_1025: PP_1025 - 2-isopropylmalate synthase, at 1,168,736 to 1,170,409 _1025 Position (kb) 1167 1168Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1166.141 kb on + strand, within PP_1022at 1166.242 kb on + strand, within PP_1022at 1166.471 kb on + strand, within PP_1022at 1166.471 kb on + strand, within PP_1022at 1166.582 kb on - strand, within PP_1022at 1166.681 kb on + strand, within PP_1022at 1166.681 kb on + strand, within PP_1022at 1166.681 kb on + strand, within PP_1022at 1166.681 kb on + strand, within PP_1022at 1166.681 kb on + strand, within PP_1022at 1166.716 kb on + strand, within PP_1022at 1166.870 kb on + strand, within PP_1022at 1166.870 kb on + strand, within PP_1022at 1166.871 kb on - strand, within PP_1022at 1166.981 kb on - strandat 1166.981 kb on - strandat 1166.981 kb on - strandat 1167.079 kb on + strandat 1168.501 kb on - strandat 1168.547 kb on + strandat 1168.547 kb on + strandat 1168.548 kb on - strandat 1168.581 kb on - strandat 1168.592 kb on + strandat 1168.641 kb on - strandat 1168.643 kb on + strandat 1168.643 kb on + strandat 1168.644 kb on - strandat 1168.644 kb on - strandat 1168.644 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH6 and (C) D-Glucose
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1,166,141 + PP_1022 0.36 -3.5
1,166,242 + PP_1022 0.43 -2.2
1,166,471 + PP_1022 0.58 -2.2
1,166,471 + PP_1022 0.58 -1.5
1,166,582 - PP_1022 0.66 +1.8
1,166,681 + PP_1022 0.72 -3.1
1,166,681 + PP_1022 0.72 -1.5
1,166,681 + PP_1022 0.72 -3.8
1,166,681 + PP_1022 0.72 -2.9
1,166,681 + PP_1022 0.72 +1.8
1,166,716 + PP_1022 0.75 -0.7
1,166,870 + PP_1022 0.85 -2.5
1,166,870 + PP_1022 0.85 -1.0
1,166,871 - PP_1022 0.85 +0.3
1,166,981 - +1.8
1,166,981 - +1.8
1,166,981 - -0.2
1,167,079 + -1.0
1,168,501 - -0.1
1,168,547 + +0.3
1,168,547 + +0.4
1,168,548 - +1.2
1,168,581 - +0.2
1,168,592 + +3.7
1,168,641 - -0.6
1,168,643 + -1.5
1,168,643 + +1.5
1,168,644 - +1.1
1,168,644 - -4.0
1,168,644 - -0.3

Or see this region's nucleotide sequence