Experiment: Growth at 25C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3415 and PP_3416 are separated by 206 nucleotides PP_3416 and PP_3417 are separated by 111 nucleotides PP_3417 and PP_3418 are separated by 86 nucleotides PP_3418 and PP_3419 are separated by 14 nucleotides
PP_3415: PP_3415 - Transcriptional regulator, LacI family, at 3,865,858 to 3,866,877
_3415
PP_3416: PP_3416 - D-gluconate kinase, at 3,867,084 to 3,867,623
_3416
PP_3417: PP_3417 - D-gluconate transporter, at 3,867,735 to 3,869,087
_3417
PP_3418: PP_3418 - conserved protein of unknown function, at 3,869,174 to 3,869,476
_3418
PP_3419: PP_3419 - Sigma-54 dependent transcriptional regulator/response regulator, at 3,869,491 to 3,870,918
_3419
Position (kb)
3867
3868
3869
3870 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3866.853 kb on + strand at 3866.854 kb on - strand at 3866.854 kb on - strand at 3866.854 kb on - strand at 3866.892 kb on + strand at 3866.921 kb on + strand at 3866.922 kb on - strand at 3866.953 kb on + strand at 3866.953 kb on + strand at 3866.953 kb on + strand at 3866.953 kb on + strand at 3866.954 kb on - strand at 3866.997 kb on + strand at 3866.998 kb on - strand at 3867.020 kb on - strand at 3867.020 kb on - strand at 3867.079 kb on + strand at 3867.079 kb on + strand at 3867.080 kb on - strand at 3867.584 kb on - strand at 3867.714 kb on + strand at 3867.715 kb on - strand at 3867.715 kb on - strand at 3867.750 kb on - strand at 3867.750 kb on - strand at 3867.783 kb on - strand at 3867.783 kb on - strand at 3867.928 kb on + strand, within PP_3417 at 3867.928 kb on + strand, within PP_3417 at 3868.287 kb on + strand, within PP_3417 at 3868.440 kb on - strand, within PP_3417 at 3868.440 kb on - strand, within PP_3417 at 3868.440 kb on - strand, within PP_3417 at 3868.453 kb on + strand, within PP_3417 at 3868.453 kb on + strand, within PP_3417 at 3868.453 kb on + strand, within PP_3417 at 3868.454 kb on - strand, within PP_3417 at 3868.856 kb on + strand, within PP_3417 at 3868.909 kb on + strand, within PP_3417 at 3868.981 kb on - strand at 3868.981 kb on - strand at 3868.981 kb on - strand at 3869.101 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.156 kb on - strand at 3869.156 kb on - strand at 3869.347 kb on - strand, within PP_3418 at 3869.467 kb on + strand at 3869.490 kb on - strand at 3869.490 kb on - strand at 3869.492 kb on + strand at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 25C; with MOPS remove 3,866,853 + +1.1 3,866,854 - -0.3 3,866,854 - -2.6 3,866,854 - +0.6 3,866,892 + +1.7 3,866,921 + -0.6 3,866,922 - -3.3 3,866,953 + -0.8 3,866,953 + +0.5 3,866,953 + -0.0 3,866,953 + -0.3 3,866,954 - +0.4 3,866,997 + -2.1 3,866,998 - -3.6 3,867,020 - -2.2 3,867,020 - -1.0 3,867,079 + +0.8 3,867,079 + -0.3 3,867,080 - -1.7 3,867,584 - -0.9 3,867,714 + -1.1 3,867,715 - -3.4 3,867,715 - -2.0 3,867,750 - -3.7 3,867,750 - +1.3 3,867,783 - -0.7 3,867,783 - -0.4 3,867,928 + PP_3417 0.14 -3.7 3,867,928 + PP_3417 0.14 +0.2 3,868,287 + PP_3417 0.41 -0.5 3,868,440 - PP_3417 0.52 -3.4 3,868,440 - PP_3417 0.52 -4.4 3,868,440 - PP_3417 0.52 -0.2 3,868,453 + PP_3417 0.53 -2.2 3,868,453 + PP_3417 0.53 -2.6 3,868,453 + PP_3417 0.53 -1.2 3,868,454 - PP_3417 0.53 -0.8 3,868,856 + PP_3417 0.83 -0.1 3,868,909 + PP_3417 0.87 -0.7 3,868,981 - -1.3 3,868,981 - -1.8 3,868,981 - -4.0 3,869,101 + -1.0 3,869,140 + -0.7 3,869,140 + +0.0 3,869,140 + -0.3 3,869,141 - -0.0 3,869,141 - -0.7 3,869,141 - -3.4 3,869,141 - -0.3 3,869,156 - -0.2 3,869,156 - +0.3 3,869,347 - PP_3418 0.57 -0.2 3,869,467 + -0.3 3,869,490 - -0.1 3,869,490 - -2.5 3,869,492 + -1.4 3,869,832 + PP_3419 0.24 +0.3 3,869,832 + PP_3419 0.24 -3.7 3,869,832 + PP_3419 0.24 -0.2 3,869,832 + PP_3419 0.24 +0.9 3,869,833 - PP_3419 0.24 +0.6 3,869,833 - PP_3419 0.24 +0.2
Or see this region's nucleotide sequence