Experiment: Growth at 25C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0297 and PP_5441 are separated by 129 nucleotides PP_5441 and PP_0298 are separated by 171 nucleotides
PP_0297: PP_0297 - L-serine dehydratase, at 356,656 to 358,032
_0297
PP_5441: PP_5441 - protein of unknown function, at 358,162 to 358,362
_5441
PP_0298: PP_0298 - AraC-family transcriptional regulator, at 358,534 to 359,640
_0298
Position (kb) 358
359 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 357.337 kb on + strand, within PP_0297 at 357.337 kb on + strand at 357.338 kb on - strand, within PP_0297 at 357.338 kb on - strand, within PP_0297 at 357.338 kb on - strand, within PP_0297 at 357.562 kb on + strand, within PP_0297 at 357.562 kb on + strand, within PP_0297 at 357.562 kb on + strand, within PP_0297 at 357.562 kb on + strand, within PP_0297 at 357.562 kb on + strand, within PP_0297 at 357.563 kb on - strand, within PP_0297 at 357.563 kb on - strand, within PP_0297 at 357.563 kb on - strand, within PP_0297 at 357.565 kb on + strand, within PP_0297 at 357.565 kb on + strand, within PP_0297 at 357.565 kb on + strand, within PP_0297 at 357.566 kb on - strand, within PP_0297 at 357.566 kb on - strand, within PP_0297 at 357.566 kb on - strand, within PP_0297 at 357.610 kb on + strand, within PP_0297 at 357.938 kb on + strand at 357.969 kb on + strand at 357.970 kb on - strand at 357.982 kb on + strand at 357.982 kb on + strand at 357.982 kb on + strand at 357.983 kb on - strand at 358.079 kb on + strand at 358.080 kb on - strand at 358.102 kb on - strand at 358.102 kb on - strand at 358.161 kb on + strand at 358.161 kb on + strand at 358.162 kb on - strand at 358.162 kb on - strand at 358.162 kb on - strand at 358.162 kb on - strand at 358.172 kb on - strand at 358.172 kb on - strand at 358.245 kb on + strand, within PP_5441 at 358.245 kb on + strand, within PP_5441 at 358.245 kb on + strand, within PP_5441 at 358.246 kb on - strand, within PP_5441 at 358.287 kb on - strand, within PP_5441 at 358.287 kb on - strand, within PP_5441 at 358.373 kb on - strand at 358.448 kb on + strand at 358.449 kb on - strand at 358.475 kb on + strand at 358.476 kb on - strand at 358.485 kb on + strand at 358.485 kb on + strand at 358.485 kb on + strand at 358.485 kb on + strand at 358.485 kb on + strand at 358.486 kb on - strand at 358.601 kb on + strand at 358.601 kb on + strand at 358.601 kb on + strand at 358.601 kb on + strand at 358.602 kb on - strand at 358.602 kb on - strand at 359.145 kb on + strand, within PP_0298 at 359.281 kb on + strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.282 kb on - strand, within PP_0298 at 359.350 kb on + strand, within PP_0298 at 359.351 kb on - strand, within PP_0298 at 359.353 kb on + strand, within PP_0298 at 359.354 kb on - strand, within PP_0298 at 359.354 kb on - strand, within PP_0298 at 359.354 kb on - strand, within PP_0298
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 25C; with MOPS remove 357,337 + PP_0297 0.49 +0.4 357,337 + +0.2 357,338 - PP_0297 0.50 -0.0 357,338 - PP_0297 0.50 +0.1 357,338 - PP_0297 0.50 -2.1 357,562 + PP_0297 0.66 -0.0 357,562 + PP_0297 0.66 -3.9 357,562 + PP_0297 0.66 +1.2 357,562 + PP_0297 0.66 -3.0 357,562 + PP_0297 0.66 +1.6 357,563 - PP_0297 0.66 -3.9 357,563 - PP_0297 0.66 -1.4 357,563 - PP_0297 0.66 +1.1 357,565 + PP_0297 0.66 -2.8 357,565 + PP_0297 0.66 -3.1 357,565 + PP_0297 0.66 -4.7 357,566 - PP_0297 0.66 -0.3 357,566 - PP_0297 0.66 -3.3 357,566 - PP_0297 0.66 -0.7 357,610 + PP_0297 0.69 +0.6 357,938 + +1.8 357,969 + +1.6 357,970 - -0.5 357,982 + -3.2 357,982 + -1.8 357,982 + +1.0 357,983 - -0.2 358,079 + -0.6 358,080 - +0.9 358,102 - -0.4 358,102 - -0.4 358,161 + -0.2 358,161 + +1.1 358,162 - +0.9 358,162 - +0.6 358,162 - +0.7 358,162 - +1.5 358,172 - +0.2 358,172 - -0.8 358,245 + PP_5441 0.41 +2.8 358,245 + PP_5441 0.41 -2.7 358,245 + PP_5441 0.41 -0.5 358,246 - PP_5441 0.42 +1.2 358,287 - PP_5441 0.62 -0.1 358,287 - PP_5441 0.62 +0.9 358,373 - +0.5 358,448 + -2.5 358,449 - -3.9 358,475 + -1.1 358,476 - -1.5 358,485 + +0.5 358,485 + -3.0 358,485 + +0.8 358,485 + -1.8 358,485 + -0.2 358,486 - -0.2 358,601 + +1.3 358,601 + +0.3 358,601 + +0.4 358,601 + +0.8 358,602 - +0.7 358,602 - -1.5 359,145 + PP_0298 0.55 -0.8 359,281 + PP_0298 0.67 -0.0 359,282 - PP_0298 0.68 +0.3 359,282 - PP_0298 0.68 +0.5 359,282 - PP_0298 0.68 +1.0 359,282 - PP_0298 0.68 -2.0 359,282 - PP_0298 0.68 -0.4 359,282 - PP_0298 0.68 -1.2 359,282 - PP_0298 0.68 -0.6 359,350 + PP_0298 0.74 +2.0 359,351 - PP_0298 0.74 -2.8 359,353 + PP_0298 0.74 -1.9 359,354 - PP_0298 0.74 +0.7 359,354 - PP_0298 0.74 -0.2 359,354 - PP_0298 0.74 +1.2
Or see this region's nucleotide sequence