Experiment: Growth at 25C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4742 and PP_5711 are separated by 10 nucleotides PP_5711 and PP_4743 are separated by 29 nucleotides
PP_4742: PP_4742 - Type I restriction-modification system, S subunit, at 5,396,989 to 5,398,719
_4742
PP_5711: PP_5711 - protein of unknown function, at 5,398,730 to 5,399,158
_5711
PP_4743: PP_4743 - conserved protein of unknown function, at 5,399,188 to 5,400,624
_4743
Position (kb)
5399
5400
5401 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5398.192 kb on - strand, within PP_4742 at 5398.256 kb on + strand, within PP_4742 at 5398.277 kb on + strand, within PP_4742 at 5398.277 kb on + strand, within PP_4742 at 5398.278 kb on - strand, within PP_4742 at 5398.326 kb on + strand, within PP_4742 at 5398.328 kb on + strand, within PP_4742 at 5398.329 kb on - strand, within PP_4742 at 5398.332 kb on - strand, within PP_4742 at 5398.400 kb on + strand, within PP_4742 at 5398.498 kb on + strand, within PP_4742 at 5398.499 kb on - strand, within PP_4742 at 5398.542 kb on + strand, within PP_4742 at 5398.650 kb on - strand at 5398.661 kb on + strand at 5398.760 kb on + strand at 5398.816 kb on - strand, within PP_5711 at 5398.872 kb on + strand, within PP_5711 at 5398.873 kb on - strand, within PP_5711 at 5398.886 kb on + strand, within PP_5711 at 5398.933 kb on + strand, within PP_5711 at 5398.966 kb on + strand, within PP_5711 at 5399.156 kb on + strand at 5399.161 kb on - strand at 5399.323 kb on - strand at 5399.410 kb on - strand, within PP_4743 at 5399.897 kb on + strand, within PP_4743 at 5400.241 kb on + strand, within PP_4743 at 5400.242 kb on - strand, within PP_4743 at 5400.242 kb on - strand, within PP_4743 at 5400.331 kb on + strand, within PP_4743 at 5400.360 kb on - strand, within PP_4743 at 5400.433 kb on - strand, within PP_4743 at 5400.563 kb on + strand at 5400.810 kb on + strand at 5400.810 kb on + strand at 5400.863 kb on + strand at 5400.864 kb on - strand at 5400.864 kb on - strand at 5400.870 kb on + strand at 5400.871 kb on - strand at 5400.898 kb on + strand at 5400.904 kb on - strand at 5400.904 kb on - strand at 5401.033 kb on - strand at 5401.058 kb on - strand at 5401.083 kb on + strand at 5401.132 kb on + strand at 5401.133 kb on - strand at 5401.157 kb on - strand at 5401.210 kb on + strand at 5401.211 kb on - strand at 5401.211 kb on - strand at 5401.211 kb on - strand at 5401.283 kb on + strand at 5401.283 kb on + strand at 5401.284 kb on - strand at 5401.313 kb on + strand at 5401.314 kb on - strand at 5401.314 kb on - strand at 5401.357 kb on + strand at 5401.358 kb on - strand at 5401.358 kb on - strand at 5401.392 kb on + strand at 5401.392 kb on + strand at 5401.392 kb on + strand at 5401.393 kb on - strand at 5401.402 kb on + strand at 5401.403 kb on - strand at 5401.429 kb on - strand at 5401.429 kb on - strand at 5401.509 kb on + strand at 5401.510 kb on - strand at 5401.510 kb on - strand at 5401.510 kb on - strand at 5401.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 25C; with MOPS remove 5,398,192 - PP_4742 0.69 -3.9 5,398,256 + PP_4742 0.73 +0.5 5,398,277 + PP_4742 0.74 +0.6 5,398,277 + PP_4742 0.74 +0.5 5,398,278 - PP_4742 0.74 +0.7 5,398,326 + PP_4742 0.77 +0.4 5,398,328 + PP_4742 0.77 +0.3 5,398,329 - PP_4742 0.77 +1.3 5,398,332 - PP_4742 0.78 +1.2 5,398,400 + PP_4742 0.82 -0.5 5,398,498 + PP_4742 0.87 -1.3 5,398,499 - PP_4742 0.87 +0.5 5,398,542 + PP_4742 0.90 +1.6 5,398,650 - +4.2 5,398,661 + +0.8 5,398,760 + -0.3 5,398,816 - PP_5711 0.20 +1.8 5,398,872 + PP_5711 0.33 -0.3 5,398,873 - PP_5711 0.33 +0.3 5,398,886 + PP_5711 0.36 -0.0 5,398,933 + PP_5711 0.47 +0.5 5,398,966 + PP_5711 0.55 +0.7 5,399,156 + -0.1 5,399,161 - +0.3 5,399,323 - +1.0 5,399,410 - PP_4743 0.15 +1.1 5,399,897 + PP_4743 0.49 +1.4 5,400,241 + PP_4743 0.73 +1.2 5,400,242 - PP_4743 0.73 +0.0 5,400,242 - PP_4743 0.73 +1.1 5,400,331 + PP_4743 0.80 +1.3 5,400,360 - PP_4743 0.82 +0.1 5,400,433 - PP_4743 0.87 -2.0 5,400,563 + -0.4 5,400,810 + +1.1 5,400,810 + +1.5 5,400,863 + +0.3 5,400,864 - -0.6 5,400,864 - -2.6 5,400,870 + +0.7 5,400,871 - +0.4 5,400,898 + -4.2 5,400,904 - +1.3 5,400,904 - +1.6 5,401,033 - +0.4 5,401,058 - +0.3 5,401,083 + -0.8 5,401,132 + -1.3 5,401,133 - -0.6 5,401,157 - +1.7 5,401,210 + +1.6 5,401,211 - -4.0 5,401,211 - +0.2 5,401,211 - +0.3 5,401,283 + +1.4 5,401,283 + -0.0 5,401,284 - -3.9 5,401,313 + -0.9 5,401,314 - -1.6 5,401,314 - -0.8 5,401,357 + +1.2 5,401,358 - -3.7 5,401,358 - +0.2 5,401,392 + -0.3 5,401,392 + -3.8 5,401,392 + -3.5 5,401,393 - +0.5 5,401,402 + +1.2 5,401,403 - -1.4 5,401,429 - +1.0 5,401,429 - +1.5 5,401,509 + +2.3 5,401,510 - -0.8 5,401,510 - +1.0 5,401,510 - -0.3 5,401,512 + +0.8
Or see this region's nucleotide sequence