Experiment: Growth at 25C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4031 and PP_4032 are separated by 146 nucleotides PP_4032 and PP_4033 are separated by 87 nucleotides PP_4033 and PP_4034 overlap by 4 nucleotides
PP_4031: PP_4031 - Na(+)/H(+) antiporter NhaB, at 4,541,284 to 4,542,786
_4031
PP_4032: PP_4032 - putative Outer membrane lipoprotein Blc, at 4,542,933 to 4,543,478
_4032
PP_4033: PP_4033 - Ribonuclease Z, at 4,543,566 to 4,544,525
_4033
PP_4034: PP_4034 - N-carbamoyl-beta-alanine amidohydrolase/allantoine amidohydrolase 2, at 4,544,522 to 4,545,805
_4034
Position (kb) 4543
4544
4545 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4542.703 kb on + strand at 4542.733 kb on + strand at 4542.734 kb on - strand at 4542.734 kb on - strand at 4542.734 kb on - strand at 4542.735 kb on + strand at 4542.736 kb on - strand at 4542.775 kb on + strand at 4542.775 kb on + strand at 4542.776 kb on - strand at 4542.867 kb on + strand at 4542.867 kb on + strand at 4542.903 kb on + strand at 4542.903 kb on + strand at 4543.044 kb on + strand, within PP_4032 at 4543.045 kb on - strand, within PP_4032 at 4543.045 kb on - strand, within PP_4032 at 4543.053 kb on - strand, within PP_4032 at 4543.094 kb on + strand, within PP_4032 at 4543.094 kb on + strand, within PP_4032 at 4543.150 kb on + strand, within PP_4032 at 4543.151 kb on - strand, within PP_4032 at 4543.151 kb on - strand, within PP_4032 at 4543.151 kb on - strand, within PP_4032 at 4543.151 kb on - strand, within PP_4032 at 4543.297 kb on - strand, within PP_4032 at 4543.309 kb on - strand, within PP_4032 at 4543.324 kb on - strand, within PP_4032 at 4543.440 kb on + strand at 4543.440 kb on + strand at 4543.441 kb on - strand at 4543.441 kb on - strand at 4543.441 kb on - strand at 4543.441 kb on - strand at 4543.441 kb on - strand at 4543.472 kb on + strand at 4543.472 kb on + strand at 4543.473 kb on - strand at 4543.509 kb on + strand at 4543.526 kb on - strand at 4543.662 kb on + strand, within PP_4033 at 4543.663 kb on - strand, within PP_4033 at 4544.006 kb on - strand, within PP_4033 at 4544.006 kb on - strand, within PP_4033 at 4544.066 kb on - strand, within PP_4033 at 4544.066 kb on - strand, within PP_4033 at 4544.115 kb on + strand, within PP_4033 at 4544.116 kb on - strand, within PP_4033 at 4544.116 kb on - strand, within PP_4033 at 4544.116 kb on - strand, within PP_4033 at 4544.116 kb on - strand, within PP_4033 at 4544.116 kb on - strand, within PP_4033 at 4544.146 kb on - strand, within PP_4033 at 4544.146 kb on - strand, within PP_4033 at 4544.146 kb on - strand, within PP_4033 at 4544.239 kb on + strand, within PP_4033 at 4544.239 kb on + strand, within PP_4033 at 4544.240 kb on - strand, within PP_4033 at 4544.359 kb on + strand, within PP_4033 at 4544.360 kb on - strand, within PP_4033 at 4544.445 kb on + strand at 4544.446 kb on - strand at 4544.485 kb on - strand at 4544.793 kb on + strand, within PP_4034 at 4545.041 kb on + strand, within PP_4034 at 4545.222 kb on + strand, within PP_4034 at 4545.223 kb on - strand, within PP_4034 at 4545.223 kb on - strand, within PP_4034 at 4545.354 kb on - strand, within PP_4034
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 25C; with MOPS remove 4,542,703 + +1.1 4,542,733 + -3.7 4,542,734 - +0.3 4,542,734 - +1.1 4,542,734 - +1.2 4,542,735 + +0.6 4,542,736 - +0.2 4,542,775 + +1.0 4,542,775 + +0.0 4,542,776 - -1.8 4,542,867 + +1.1 4,542,867 + +1.2 4,542,903 + -0.0 4,542,903 + -0.7 4,543,044 + PP_4032 0.20 +1.9 4,543,045 - PP_4032 0.21 +0.6 4,543,045 - PP_4032 0.21 +0.8 4,543,053 - PP_4032 0.22 -2.3 4,543,094 + PP_4032 0.29 +1.2 4,543,094 + PP_4032 0.29 -0.6 4,543,150 + PP_4032 0.40 +0.7 4,543,151 - PP_4032 0.40 +1.5 4,543,151 - PP_4032 0.40 -0.0 4,543,151 - PP_4032 0.40 +1.1 4,543,151 - PP_4032 0.40 +0.3 4,543,297 - PP_4032 0.67 -0.4 4,543,309 - PP_4032 0.69 -0.3 4,543,324 - PP_4032 0.72 -0.8 4,543,440 + +0.7 4,543,440 + +0.4 4,543,441 - +1.3 4,543,441 - +0.6 4,543,441 - +1.5 4,543,441 - +1.0 4,543,441 - +1.5 4,543,472 + +0.9 4,543,472 + -3.5 4,543,473 - +0.9 4,543,509 + -3.9 4,543,526 - +0.7 4,543,662 + PP_4033 0.10 +0.6 4,543,663 - PP_4033 0.10 +0.7 4,544,006 - PP_4033 0.46 +1.0 4,544,006 - PP_4033 0.46 -0.9 4,544,066 - PP_4033 0.52 -2.4 4,544,066 - PP_4033 0.52 -0.0 4,544,115 + PP_4033 0.57 -3.8 4,544,116 - PP_4033 0.57 +0.3 4,544,116 - PP_4033 0.57 -4.4 4,544,116 - PP_4033 0.57 -0.9 4,544,116 - PP_4033 0.57 -0.7 4,544,116 - PP_4033 0.57 -1.9 4,544,146 - PP_4033 0.60 -0.4 4,544,146 - PP_4033 0.60 -1.5 4,544,146 - PP_4033 0.60 -2.8 4,544,239 + PP_4033 0.70 +0.4 4,544,239 + PP_4033 0.70 -1.4 4,544,240 - PP_4033 0.70 +1.3 4,544,359 + PP_4033 0.83 -0.3 4,544,360 - PP_4033 0.83 -1.5 4,544,445 + +0.7 4,544,446 - -0.5 4,544,485 - +0.2 4,544,793 + PP_4034 0.21 -0.4 4,545,041 + PP_4034 0.40 +0.7 4,545,222 + PP_4034 0.55 +0.4 4,545,223 - PP_4034 0.55 +3.4 4,545,223 - PP_4034 0.55 -0.9 4,545,354 - PP_4034 0.65 -0.7
Or see this region's nucleotide sequence