Strain Fitness in Pseudomonas putida KT2440 around PP_5525

Experiment: Growth at 30C; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2462 and PP_2463 are separated by 200 nucleotidesPP_2463 and PP_5525 are separated by 162 nucleotidesPP_5525 and PP_2465 are separated by 338 nucleotides PP_2462: PP_2462 - conserved protein of unknown function, at 2,808,000 to 2,808,374 _2462 PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787 _2463 PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255 _5525 PP_2465: PP_2465 - Threonine--tRNA ligase, at 2,809,594 to 2,811,516 _2465 Position (kb) 2808 2809 2810Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2808.073 kb on - strand, within PP_2462at 2808.073 kb on - strand, within PP_2462at 2808.431 kb on + strandat 2808.431 kb on + strandat 2808.431 kb on + strandat 2808.431 kb on + strandat 2808.432 kb on - strandat 2808.480 kb on + strandat 2808.567 kb on + strandat 2808.567 kb on + strandat 2808.568 kb on - strandat 2808.693 kb on - strand, within PP_2463at 2808.808 kb on + strandat 2808.809 kb on - strandat 2808.866 kb on + strandat 2808.880 kb on + strandat 2808.880 kb on + strandat 2808.890 kb on + strandat 2808.891 kb on - strandat 2808.944 kb on - strandat 2809.155 kb on + strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.263 kb on + strandat 2809.264 kb on - strandat 2809.292 kb on + strandat 2809.292 kb on + strandat 2809.293 kb on - strandat 2809.332 kb on - strandat 2809.332 kb on - strandat 2809.389 kb on + strandat 2809.389 kb on + strandat 2809.430 kb on + strandat 2809.533 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at 30C; with MOPS
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2,808,073 - PP_2462 0.19 +0.6
2,808,073 - PP_2462 0.19 +1.5
2,808,431 + +0.2
2,808,431 + -0.9
2,808,431 + +0.5
2,808,431 + -0.2
2,808,432 - +0.2
2,808,480 + +0.1
2,808,567 + +1.2
2,808,567 + -0.5
2,808,568 - +0.5
2,808,693 - PP_2463 0.55 +2.7
2,808,808 + +1.3
2,808,809 - +1.0
2,808,866 + +0.7
2,808,880 + -0.5
2,808,880 + -2.2
2,808,890 + -4.1
2,808,891 - -1.0
2,808,944 - -0.5
2,809,155 + PP_5525 0.67 +1.8
2,809,156 - PP_5525 0.67 +0.9
2,809,156 - PP_5525 0.67 +1.5
2,809,156 - PP_5525 0.67 -2.6
2,809,156 - PP_5525 0.67 +1.0
2,809,156 - PP_5525 0.67 -0.7
2,809,263 + +1.4
2,809,264 - +1.0
2,809,292 + +1.1
2,809,292 + -1.4
2,809,293 - +1.1
2,809,332 - -1.8
2,809,332 - -3.9
2,809,389 + -0.7
2,809,389 + -0.6
2,809,430 + +0.3
2,809,533 + +0.9

Or see this region's nucleotide sequence