Experiment: Growth at 30C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0638 and PP_5448 are separated by 793 nucleotides PP_5448 and PP_5449 are separated by 65 nucleotides
PP_0638: PP_0638 - transposase, at 746,517 to 746,873
_0638
PP_5448: PP_5448 - conserved protein of unknown function, at 747,667 to 747,882
_5448
PP_5449: PP_5449 - conserved exported protein of unknown function, at 747,948 to 748,766
_5449
Position (kb) 747
748 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 746.965 kb on + strand at 746.965 kb on + strand at 746.984 kb on + strand at 746.984 kb on + strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 747.037 kb on + strand at 747.037 kb on + strand at 747.038 kb on - strand at 747.063 kb on + strand at 747.064 kb on - strand at 747.064 kb on - strand at 747.168 kb on + strand at 747.230 kb on - strand at 747.259 kb on + strand at 747.259 kb on + strand at 747.259 kb on + strand at 747.268 kb on - strand at 747.305 kb on - strand at 747.305 kb on - strand at 747.367 kb on + strand at 747.368 kb on - strand at 747.405 kb on + strand at 747.405 kb on + strand at 747.405 kb on + strand at 747.406 kb on - strand at 747.497 kb on + strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.511 kb on + strand at 747.511 kb on + strand at 747.512 kb on - strand at 747.683 kb on + strand at 747.720 kb on + strand, within PP_5448 at 747.720 kb on + strand, within PP_5448 at 747.721 kb on - strand, within PP_5448 at 747.787 kb on + strand, within PP_5448 at 747.792 kb on + strand, within PP_5448 at 747.792 kb on + strand, within PP_5448 at 747.886 kb on + strand at 747.954 kb on - strand at 747.977 kb on - strand at 748.034 kb on + strand, within PP_5449 at 748.034 kb on + strand, within PP_5449 at 748.035 kb on - strand, within PP_5449 at 748.035 kb on - strand, within PP_5449 at 748.039 kb on - strand, within PP_5449 at 748.039 kb on - strand, within PP_5449 at 748.110 kb on - strand, within PP_5449 at 748.127 kb on + strand, within PP_5449 at 748.253 kb on + strand, within PP_5449 at 748.312 kb on - strand, within PP_5449 at 748.328 kb on - strand, within PP_5449 at 748.494 kb on - strand, within PP_5449 at 748.566 kb on + strand, within PP_5449 at 748.738 kb on - strand at 748.773 kb on - strand at 748.812 kb on + strand at 748.812 kb on + strand at 748.879 kb on + strand at 748.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 30C; with MOPS remove 746,965 + -1.1 746,965 + +1.4 746,984 + -3.7 746,984 + -1.4 746,985 - -1.1 746,985 - +1.9 746,985 - +0.8 746,985 - -3.6 746,985 - +1.3 746,985 - +1.3 746,985 - +0.3 747,037 + +0.0 747,037 + -4.0 747,038 - -0.2 747,063 + +0.3 747,064 - -3.9 747,064 - -0.6 747,168 + +0.1 747,230 - -2.8 747,259 + -2.2 747,259 + +0.1 747,259 + +1.2 747,268 - -3.3 747,305 - +0.3 747,305 - -3.8 747,367 + +0.9 747,368 - -1.4 747,405 + +0.8 747,405 + -1.6 747,405 + -3.3 747,406 - -4.2 747,497 + -0.5 747,498 - -0.7 747,498 - +1.0 747,498 - -2.3 747,498 - -0.8 747,511 + -1.0 747,511 + +1.0 747,512 - -1.1 747,683 + -1.1 747,720 + PP_5448 0.25 +1.9 747,720 + PP_5448 0.25 -0.1 747,721 - PP_5448 0.25 +0.1 747,787 + PP_5448 0.56 +2.2 747,792 + PP_5448 0.58 +1.3 747,792 + PP_5448 0.58 -0.0 747,886 + +1.0 747,954 - +0.3 747,977 - +1.4 748,034 + PP_5449 0.11 +1.5 748,034 + PP_5449 0.11 +2.2 748,035 - PP_5449 0.11 +0.1 748,035 - PP_5449 0.11 -3.0 748,039 - PP_5449 0.11 -3.3 748,039 - PP_5449 0.11 -3.4 748,110 - PP_5449 0.20 -1.0 748,127 + PP_5449 0.22 -2.8 748,253 + PP_5449 0.37 -0.7 748,312 - PP_5449 0.44 +0.3 748,328 - PP_5449 0.46 -2.8 748,494 - PP_5449 0.67 +1.1 748,566 + PP_5449 0.75 +1.2 748,738 - +0.9 748,773 - -3.0 748,812 + +0.2 748,812 + -1.9 748,879 + -0.7 748,880 - +1.3
Or see this region's nucleotide sequence