Strain Fitness in Pseudomonas putida KT2440 around PP_1105

Experiment: Growth at 30C; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1104 and PP_1105 are separated by 113 nucleotidesPP_1105 and PP_1106 overlap by 4 nucleotides PP_1104: PP_1104 - AotO-related protein, at 1,261,519 to 1,262,634 _1104 PP_1105: PP_1105 - putative DNA ligase, ATP-dependent, at 1,262,748 to 1,264,406 _1105 PP_1106: PP_1106 - conserved protein of unknown function, at 1,264,403 to 1,265,419 _1106 Position (kb) 1262 1263 1264 1265Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1261.792 kb on + strand, within PP_1104at 1262.057 kb on + strand, within PP_1104at 1262.102 kb on + strand, within PP_1104at 1262.102 kb on + strand, within PP_1104at 1262.103 kb on - strand, within PP_1104at 1262.103 kb on - strand, within PP_1104at 1262.103 kb on - strand, within PP_1104at 1262.148 kb on + strand, within PP_1104at 1262.148 kb on + strand, within PP_1104at 1262.176 kb on + strand, within PP_1104at 1262.177 kb on - strand, within PP_1104at 1262.237 kb on + strand, within PP_1104at 1262.237 kb on + strand, within PP_1104at 1262.425 kb on + strand, within PP_1104at 1262.425 kb on + strand, within PP_1104at 1262.425 kb on + strand, within PP_1104at 1262.425 kb on + strand, within PP_1104at 1262.425 kb on + strand, within PP_1104at 1262.426 kb on - strand, within PP_1104at 1262.492 kb on - strandat 1262.536 kb on - strandat 1262.536 kb on - strandat 1262.537 kb on + strandat 1262.691 kb on - strandat 1262.696 kb on + strandat 1262.696 kb on + strandat 1262.696 kb on + strandat 1262.697 kb on - strandat 1262.697 kb on - strandat 1262.697 kb on - strandat 1262.697 kb on - strandat 1262.742 kb on - strandat 1262.758 kb on - strandat 1262.844 kb on - strandat 1262.998 kb on + strand, within PP_1105at 1262.999 kb on - strand, within PP_1105at 1263.067 kb on + strand, within PP_1105at 1263.068 kb on - strand, within PP_1105at 1263.112 kb on + strand, within PP_1105at 1263.112 kb on + strand, within PP_1105at 1263.194 kb on - strand, within PP_1105at 1263.194 kb on - strand, within PP_1105at 1263.410 kb on - strand, within PP_1105at 1263.838 kb on + strand, within PP_1105at 1263.838 kb on + strand, within PP_1105at 1263.839 kb on - strand, within PP_1105at 1263.864 kb on + strand, within PP_1105at 1263.864 kb on + strand, within PP_1105at 1263.864 kb on + strand, within PP_1105at 1263.864 kb on + strand, within PP_1105at 1263.865 kb on - strand, within PP_1105at 1263.865 kb on - strand, within PP_1105at 1263.865 kb on - strand, within PP_1105at 1263.865 kb on - strand, within PP_1105at 1263.865 kb on - strand, within PP_1105at 1263.865 kb on - strand, within PP_1105at 1263.874 kb on + strand, within PP_1105at 1263.993 kb on + strand, within PP_1105at 1263.993 kb on + strand, within PP_1105at 1263.994 kb on - strand, within PP_1105at 1263.994 kb on - strand, within PP_1105at 1264.181 kb on - strand, within PP_1105at 1264.181 kb on - strand, within PP_1105at 1264.236 kb on + strand, within PP_1105at 1264.237 kb on - strand, within PP_1105at 1264.237 kb on - strand, within PP_1105at 1264.328 kb on - strandat 1264.391 kb on + strandat 1264.392 kb on - strandat 1264.446 kb on + strandat 1264.492 kb on - strandat 1264.492 kb on - strandat 1264.502 kb on + strandat 1264.502 kb on + strandat 1264.502 kb on + strandat 1264.503 kb on - strandat 1264.672 kb on - strand, within PP_1106at 1264.697 kb on - strand, within PP_1106at 1264.697 kb on - strand, within PP_1106at 1264.763 kb on - strand, within PP_1106at 1264.905 kb on + strand, within PP_1106at 1264.905 kb on + strand, within PP_1106at 1264.906 kb on - strand, within PP_1106at 1265.062 kb on + strand, within PP_1106at 1265.062 kb on + strand, within PP_1106at 1265.063 kb on - strand, within PP_1106at 1265.081 kb on + strand, within PP_1106at 1265.081 kb on + strand, within PP_1106at 1265.088 kb on + strand, within PP_1106at 1265.088 kb on + strand, within PP_1106at 1265.089 kb on - strand, within PP_1106at 1265.089 kb on - strand, within PP_1106at 1265.089 kb on - strand, within PP_1106at 1265.209 kb on - strand, within PP_1106at 1265.283 kb on + strand, within PP_1106at 1265.284 kb on - strand, within PP_1106at 1265.365 kb on - strandat 1265.385 kb on + strandat 1265.385 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at 30C; with MOPS
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1,261,792 + PP_1104 0.24 +1.9
1,262,057 + PP_1104 0.48 +1.8
1,262,102 + PP_1104 0.52 -3.5
1,262,102 + PP_1104 0.52 +1.6
1,262,103 - PP_1104 0.52 +0.9
1,262,103 - PP_1104 0.52 +2.0
1,262,103 - PP_1104 0.52 +0.8
1,262,148 + PP_1104 0.56 -3.9
1,262,148 + PP_1104 0.56 +1.0
1,262,176 + PP_1104 0.59 +1.4
1,262,177 - PP_1104 0.59 +0.4
1,262,237 + PP_1104 0.64 +0.9
1,262,237 + PP_1104 0.64 +0.8
1,262,425 + PP_1104 0.81 +0.2
1,262,425 + PP_1104 0.81 -0.2
1,262,425 + PP_1104 0.81 +0.4
1,262,425 + PP_1104 0.81 +0.4
1,262,425 + PP_1104 0.81 -2.4
1,262,426 - PP_1104 0.81 -0.7
1,262,492 - +1.1
1,262,536 - -0.0
1,262,536 - -4.1
1,262,537 + -0.5
1,262,691 - +0.1
1,262,696 + -0.8
1,262,696 + +0.0
1,262,696 + -3.8
1,262,697 - -1.4
1,262,697 - -0.2
1,262,697 - -1.9
1,262,697 - +3.1
1,262,742 - -0.7
1,262,758 - +1.0
1,262,844 - -1.9
1,262,998 + PP_1105 0.15 -3.7
1,262,999 - PP_1105 0.15 -4.1
1,263,067 + PP_1105 0.19 -2.7
1,263,068 - PP_1105 0.19 +0.4
1,263,112 + PP_1105 0.22 +0.5
1,263,112 + PP_1105 0.22 +0.9
1,263,194 - PP_1105 0.27 +0.6
1,263,194 - PP_1105 0.27 +0.1
1,263,410 - PP_1105 0.40 -2.1
1,263,838 + PP_1105 0.66 +0.3
1,263,838 + PP_1105 0.66 +0.7
1,263,839 - PP_1105 0.66 +0.3
1,263,864 + PP_1105 0.67 -0.1
1,263,864 + PP_1105 0.67 -0.0
1,263,864 + PP_1105 0.67 +1.4
1,263,864 + PP_1105 0.67 +1.1
1,263,865 - PP_1105 0.67 -0.7
1,263,865 - PP_1105 0.67 -0.9
1,263,865 - PP_1105 0.67 -3.2
1,263,865 - PP_1105 0.67 -1.2
1,263,865 - PP_1105 0.67 -0.1
1,263,865 - PP_1105 0.67 -0.6
1,263,874 + PP_1105 0.68 -1.0
1,263,993 + PP_1105 0.75 -3.7
1,263,993 + PP_1105 0.75 -3.5
1,263,994 - PP_1105 0.75 -2.1
1,263,994 - PP_1105 0.75 +0.0
1,264,181 - PP_1105 0.86 +0.3
1,264,181 - PP_1105 0.86 +0.7
1,264,236 + PP_1105 0.90 +0.0
1,264,237 - PP_1105 0.90 -3.8
1,264,237 - PP_1105 0.90 -0.8
1,264,328 - +0.0
1,264,391 + +1.4
1,264,392 - -0.1
1,264,446 + +0.1
1,264,492 - -1.7
1,264,492 - +0.5
1,264,502 + -0.4
1,264,502 + -0.8
1,264,502 + -0.8
1,264,503 - -1.6
1,264,672 - PP_1106 0.26 -3.5
1,264,697 - PP_1106 0.29 +0.6
1,264,697 - PP_1106 0.29 +0.6
1,264,763 - PP_1106 0.35 -2.4
1,264,905 + PP_1106 0.49 -0.8
1,264,905 + PP_1106 0.49 +1.3
1,264,906 - PP_1106 0.49 +1.3
1,265,062 + PP_1106 0.65 -0.3
1,265,062 + PP_1106 0.65 -2.5
1,265,063 - PP_1106 0.65 +1.7
1,265,081 + PP_1106 0.67 -0.1
1,265,081 + PP_1106 0.67 -0.3
1,265,088 + PP_1106 0.67 +2.0
1,265,088 + PP_1106 0.67 -1.9
1,265,089 - PP_1106 0.67 -3.3
1,265,089 - PP_1106 0.67 +0.7
1,265,089 - PP_1106 0.67 +1.0
1,265,209 - PP_1106 0.79 -1.9
1,265,283 + PP_1106 0.87 -0.9
1,265,284 - PP_1106 0.87 -0.9
1,265,365 - -0.7
1,265,385 + -3.5
1,265,385 + +1.7

Or see this region's nucleotide sequence