Experiment: Growth at 30C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1098 and PP_1099 are separated by 167 nucleotides PP_1099 and PP_1100 are separated by 327 nucleotides PP_1100 and PP_1101 are separated by 61 nucleotides
PP_1098: PP_1098 - ATP-dependent Fe-S cluster transferase, at 1,255,659 to 1,256,753
_1098
PP_1099: PP_1099 - Cold shock protein CapB, at 1,256,921 to 1,257,130
_1099
PP_1100: PP_1100 - Deoxycytidine triphosphate deaminase, at 1,257,458 to 1,258,024
_1100
PP_1101: PP_1101 - conserved protein of unknown function, at 1,258,086 to 1,258,346
_1101
Position (kb) 1256
1257
1258 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1256.795 kb on - strand at 1256.853 kb on - strand at 1256.904 kb on + strand at 1256.904 kb on + strand at 1256.912 kb on + strand at 1256.913 kb on - strand at 1256.981 kb on - strand, within PP_1099 at 1257.103 kb on + strand, within PP_1099 at 1257.104 kb on - strand, within PP_1099 at 1257.104 kb on - strand, within PP_1099 at 1257.104 kb on - strand, within PP_1099 at 1257.243 kb on - strand at 1257.388 kb on - strand at 1257.411 kb on + strand at 1257.411 kb on + strand at 1257.584 kb on + strand at 1257.591 kb on - strand, within PP_1100 at 1257.591 kb on - strand at 1257.747 kb on - strand, within PP_1100 at 1257.797 kb on + strand at 1257.966 kb on - strand at 1257.987 kb on - strand at 1258.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 30C; with MOPS remove 1,256,795 - +0.8 1,256,853 - +1.8 1,256,904 + +1.5 1,256,904 + -0.4 1,256,912 + -0.1 1,256,913 - +0.0 1,256,981 - PP_1099 0.29 +1.1 1,257,103 + PP_1099 0.87 -0.9 1,257,104 - PP_1099 0.87 +1.7 1,257,104 - PP_1099 0.87 +0.7 1,257,104 - PP_1099 0.87 -0.9 1,257,243 - -0.3 1,257,388 - +0.3 1,257,411 + -2.2 1,257,411 + +3.0 1,257,584 + -0.4 1,257,591 - PP_1100 0.23 -0.4 1,257,591 - +0.9 1,257,747 - PP_1100 0.51 -1.4 1,257,797 + +0.9 1,257,966 - +0.3 1,257,987 - +1.9 1,258,072 + +0.5
Or see this region's nucleotide sequence