Strain Fitness in Pseudomonas putida KT2440 around PP_3225

Experiment: Temperature shift 10_to_25; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_3223 and PP_3224 are separated by 74 nucleotidesPP_3224 and PP_3225 are separated by 15 nucleotidesPP_3225 and PP_3226 overlap by 4 nucleotides PP_3223: PP_3223 - ABC transporter, periplasmic binding protein, at 3,658,246 to 3,659,865 _3223 PP_3224: PP_3224 - Class II aldolase/adducin domain protein, at 3,659,940 to 3,660,734 _3224 PP_3225: PP_3225 - D-isomer specific 2-hydroxyacid dehydrogenase family protein, at 3,660,750 to 3,661,694 _3225 PP_3226: PP_3226 - Acyl-CoA dehydrogenase-related protein, at 3,661,691 to 3,662,833 _3226 Position (kb) 3660 3661 3662Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3660.005 kb on + strandat 3660.078 kb on + strand, within PP_3224at 3660.078 kb on + strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.278 kb on - strand, within PP_3224at 3660.286 kb on + strand, within PP_3224at 3660.287 kb on - strand, within PP_3224at 3660.301 kb on + strand, within PP_3224at 3660.304 kb on + strand, within PP_3224at 3660.304 kb on + strand, within PP_3224at 3660.305 kb on - strand, within PP_3224at 3660.436 kb on + strand, within PP_3224at 3660.598 kb on + strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.637 kb on + strand, within PP_3224at 3660.901 kb on + strand, within PP_3225at 3660.901 kb on + strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3661.052 kb on - strand, within PP_3225at 3661.161 kb on + strand, within PP_3225at 3661.339 kb on - strand, within PP_3225at 3661.564 kb on - strand, within PP_3225at 3661.775 kb on + strandat 3661.979 kb on + strand, within PP_3226at 3661.980 kb on - strand, within PP_3226at 3661.980 kb on - strand, within PP_3226at 3662.088 kb on + strand, within PP_3226at 3662.089 kb on - strand, within PP_3226at 3662.089 kb on - strand, within PP_3226at 3662.091 kb on - strand, within PP_3226at 3662.121 kb on - strand, within PP_3226at 3662.343 kb on + strand, within PP_3226at 3662.373 kb on + strand, within PP_3226at 3662.577 kb on + strand, within PP_3226at 3662.578 kb on - strand, within PP_3226at 3662.685 kb on - strand, within PP_3226

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Per-strain Table

Position Strand Gene LocusTag Fraction Temperature shift 10_to_25; with MOPS
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3,660,005 + -0.2
3,660,078 + PP_3224 0.17 +0.8
3,660,078 + PP_3224 0.17 -0.1
3,660,079 - PP_3224 0.17 +0.0
3,660,079 - PP_3224 0.17 -3.3
3,660,079 - PP_3224 0.17 +0.7
3,660,079 - PP_3224 0.17 +0.2
3,660,079 - PP_3224 0.17 +0.2
3,660,278 - PP_3224 0.43 -1.9
3,660,286 + PP_3224 0.44 +0.5
3,660,287 - PP_3224 0.44 -0.5
3,660,301 + PP_3224 0.45 +1.2
3,660,304 + PP_3224 0.46 -2.5
3,660,304 + PP_3224 0.46 -3.0
3,660,305 - PP_3224 0.46 -3.6
3,660,436 + PP_3224 0.62 +0.1
3,660,598 + PP_3224 0.83 -0.7
3,660,599 - PP_3224 0.83 -4.2
3,660,599 - PP_3224 0.83 -2.1
3,660,599 - PP_3224 0.83 +1.3
3,660,599 - PP_3224 0.83 -2.2
3,660,637 + PP_3224 0.88 +0.3
3,660,901 + PP_3225 0.16 -1.2
3,660,901 + PP_3225 0.16 +0.9
3,660,902 - PP_3225 0.16 -3.8
3,660,902 - PP_3225 0.16 +0.4
3,660,902 - PP_3225 0.16 +0.3
3,661,052 - PP_3225 0.32 -0.9
3,661,161 + PP_3225 0.43 -3.9
3,661,339 - PP_3225 0.62 -2.2
3,661,564 - PP_3225 0.86 -3.6
3,661,775 + -3.7
3,661,979 + PP_3226 0.25 -0.9
3,661,980 - PP_3226 0.25 +0.8
3,661,980 - PP_3226 0.25 +0.4
3,662,088 + PP_3226 0.35 +0.1
3,662,089 - PP_3226 0.35 +0.4
3,662,089 - PP_3226 0.35 -2.9
3,662,091 - PP_3226 0.35 -3.2
3,662,121 - PP_3226 0.38 -1.1
3,662,343 + PP_3226 0.57 +0.1
3,662,373 + PP_3226 0.60 +0.5
3,662,577 + PP_3226 0.78 +0.1
3,662,578 - PP_3226 0.78 -0.4
3,662,685 - PP_3226 0.87 -1.0

Or see this region's nucleotide sequence