Strain Fitness in Pseudomonas putida KT2440 around PP_0292

Experiment: Temperature shift 10_to_25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0289 and PP_0290 are separated by 0 nucleotidesPP_0290 and PP_0291 are separated by 0 nucleotidesPP_0291 and PP_0292 are separated by 27 nucleotidesPP_0292 and PP_0293 are separated by 10 nucleotidesPP_0293 and PP_0294 are separated by 117 nucleotides PP_0289: PP_0289 - Imidazoleglycerol-phosphate dehydratase, at 349,955 to 350,587 _0289 PP_0290: PP_0290 - Imidazole glycerol phosphate synthase subunit HisH, at 350,588 to 351,226 _0290 PP_0291: PP_0291 - conserved protein of unknown function, at 351,227 to 351,487 _0291 PP_0292: PP_0292 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data), at 351,515 to 352,252 _0292 PP_0293: PP_0293 - Imidazole glycerol phosphate synthase subunit HisF, at 352,263 to 353,033 _0293 PP_0294: PP_0294 - Choline / carnitine / glycine betaine ABC transporter, ATPase component (from data), at 353,151 to 354,329 _0294 Position (kb) 351 352 353Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 350.548 kb on + strandat 350.548 kb on + strandat 350.548 kb on + strandat 350.549 kb on - strandat 350.585 kb on + strandat 350.612 kb on + strandat 350.658 kb on + strand, within PP_0290at 350.658 kb on + strand, within PP_0290at 350.659 kb on - strand, within PP_0290at 350.659 kb on - strand, within PP_0290at 350.668 kb on + strand, within PP_0290at 350.680 kb on + strand, within PP_0290at 351.035 kb on + strand, within PP_0290at 351.050 kb on + strand, within PP_0290at 351.050 kb on + strand, within PP_0290at 351.051 kb on - strand, within PP_0290at 351.051 kb on - strand, within PP_0290at 351.051 kb on - strand, within PP_0290at 351.053 kb on + strand, within PP_0290at 351.053 kb on + strand, within PP_0290at 351.053 kb on + strand, within PP_0290at 351.054 kb on - strand, within PP_0290at 351.054 kb on - strand, within PP_0290at 351.098 kb on + strand, within PP_0290at 351.099 kb on - strand, within PP_0290at 351.224 kb on + strandat 351.224 kb on + strandat 351.225 kb on - strandat 351.396 kb on - strand, within PP_0291at 351.396 kb on - strand, within PP_0291at 351.398 kb on + strand, within PP_0291at 351.398 kb on + strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.499 kb on + strandat 351.499 kb on + strandat 351.523 kb on + strandat 351.524 kb on - strandat 351.590 kb on - strand, within PP_0292at 351.791 kb on + strand, within PP_0292at 351.792 kb on - strand, within PP_0292at 351.792 kb on - strand, within PP_0292at 351.792 kb on - strand, within PP_0292at 351.812 kb on + strandat 351.813 kb on - strand, within PP_0292at 351.813 kb on - strand, within PP_0292at 352.193 kb on + strandat 352.193 kb on + strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.241 kb on + strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.457 kb on + strand, within PP_0293at 352.457 kb on + strand, within PP_0293at 352.457 kb on + strand, within PP_0293at 352.977 kb on + strandat 352.977 kb on + strandat 352.978 kb on - strandat 353.001 kb on + strandat 353.002 kb on - strandat 353.002 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Temperature shift 10_to_25
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350,548 + -3.3
350,548 + -0.9
350,548 + -3.6
350,549 - -2.7
350,585 + -2.1
350,612 + -1.8
350,658 + PP_0290 0.11 -2.4
350,658 + PP_0290 0.11 -4.7
350,659 - PP_0290 0.11 -2.9
350,659 - PP_0290 0.11 -2.7
350,668 + PP_0290 0.13 -3.1
350,680 + PP_0290 0.14 -3.3
351,035 + PP_0290 0.70 -3.2
351,050 + PP_0290 0.72 -3.7
351,050 + PP_0290 0.72 -2.8
351,051 - PP_0290 0.72 -4.1
351,051 - PP_0290 0.72 -2.9
351,051 - PP_0290 0.72 -0.9
351,053 + PP_0290 0.73 -4.1
351,053 + PP_0290 0.73 -2.9
351,053 + PP_0290 0.73 -3.6
351,054 - PP_0290 0.73 -2.9
351,054 - PP_0290 0.73 -3.7
351,098 + PP_0290 0.80 -1.7
351,099 - PP_0290 0.80 -2.9
351,224 + -2.1
351,224 + +0.8
351,225 - -2.8
351,396 - PP_0291 0.65 -3.9
351,396 - PP_0291 0.65 -3.3
351,398 + PP_0291 0.66 -0.1
351,398 + PP_0291 0.66 +0.2
351,399 - PP_0291 0.66 -1.4
351,399 - PP_0291 0.66 -3.4
351,399 - PP_0291 0.66 -2.4
351,399 - PP_0291 0.66 -3.6
351,499 + -2.1
351,499 + -4.5
351,523 + -1.4
351,524 - -2.9
351,590 - PP_0292 0.10 -2.4
351,791 + PP_0292 0.37 -3.5
351,792 - PP_0292 0.38 -2.7
351,792 - PP_0292 0.38 -3.2
351,792 - PP_0292 0.38 -3.4
351,812 + +0.5
351,813 - PP_0292 0.40 -3.5
351,813 - PP_0292 0.40 -3.7
352,193 + -2.7
352,193 + -3.8
352,194 - -2.8
352,194 - -3.5
352,194 - -2.8
352,194 - -3.9
352,241 + +0.2
352,242 - -3.0
352,242 - -2.9
352,242 - -2.5
352,242 - -3.4
352,242 - -2.8
352,242 - -2.5
352,457 + PP_0293 0.25 -3.8
352,457 + PP_0293 0.25 -1.8
352,457 + PP_0293 0.25 -2.0
352,977 + -2.4
352,977 + -2.6
352,978 - -2.8
353,001 + -2.9
353,002 - -3.0
353,002 - -3.5

Or see this region's nucleotide sequence