Experiment: Temperature shift 30_to_25; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4338 and PP_4339 are separated by 12 nucleotides PP_4339 and PP_4340 are separated by 22 nucleotides PP_4340 and PP_4341 are separated by 107 nucleotides
PP_4338: PP_4338 - Chemotaxis histidine kinase CheA, at 4,927,928 to 4,930,171
_4338
PP_4339: PP_4339 - Protein phosphatase CheZ, at 4,930,184 to 4,930,972
_4339
PP_4340: PP_4340 - response regulator for chemotactic signal transduction, at 4,930,995 to 4,931,369
_4340
PP_4341: PP_4341 - RNA polymerase sigma 28 (sigma F) factor for class III flagellar operons, at 4,931,477 to 4,932,217
_4341
Position (kb) 4930
4931 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4929.260 kb on + strand, within PP_4338 at 4929.260 kb on + strand, within PP_4338 at 4929.261 kb on - strand, within PP_4338 at 4929.261 kb on - strand, within PP_4338 at 4930.019 kb on + strand at 4930.019 kb on + strand at 4930.019 kb on + strand at 4930.019 kb on + strand at 4930.019 kb on + strand at 4930.020 kb on - strand at 4930.020 kb on - strand at 4930.020 kb on - strand at 4930.020 kb on - strand at 4930.112 kb on + strand at 4930.113 kb on - strand at 4930.128 kb on - strand at 4930.185 kb on + strand at 4930.186 kb on - strand at 4930.249 kb on + strand at 4930.334 kb on + strand, within PP_4339 at 4930.335 kb on - strand, within PP_4339 at 4930.450 kb on - strand, within PP_4339 at 4930.450 kb on - strand, within PP_4339 at 4930.701 kb on + strand, within PP_4339 at 4930.701 kb on + strand, within PP_4339 at 4930.701 kb on + strand, within PP_4339 at 4930.701 kb on + strand, within PP_4339 at 4930.701 kb on + strand, within PP_4339 at 4930.701 kb on + strand, within PP_4339 at 4930.702 kb on - strand, within PP_4339 at 4930.702 kb on - strand, within PP_4339 at 4930.702 kb on - strand, within PP_4339 at 4930.702 kb on - strand, within PP_4339 at 4930.702 kb on - strand, within PP_4339 at 4930.726 kb on + strand, within PP_4339 at 4930.726 kb on + strand, within PP_4339 at 4931.068 kb on + strand, within PP_4340 at 4931.068 kb on + strand, within PP_4340 at 4931.068 kb on + strand, within PP_4340 at 4931.233 kb on + strand, within PP_4340 at 4931.234 kb on - strand, within PP_4340 at 4931.383 kb on + strand at 4931.383 kb on + strand at 4931.384 kb on - strand at 4931.416 kb on + strand at 4931.444 kb on - strand at 4931.444 kb on - strand at 4931.605 kb on - strand, within PP_4341 at 4931.605 kb on - strand, within PP_4341 at 4931.607 kb on + strand, within PP_4341 at 4931.608 kb on - strand, within PP_4341 at 4931.812 kb on - strand, within PP_4341 at 4931.812 kb on - strand, within PP_4341 at 4931.814 kb on + strand, within PP_4341 at 4931.814 kb on + strand, within PP_4341 at 4931.814 kb on + strand, within PP_4341 at 4931.814 kb on + strand, within PP_4341 at 4931.815 kb on - strand, within PP_4341 at 4931.911 kb on + strand, within PP_4341 at 4931.928 kb on - strand, within PP_4341
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_25; with MOPS remove 4,929,260 + PP_4338 0.59 -4.6 4,929,260 + PP_4338 0.59 -3.1 4,929,261 - PP_4338 0.59 -3.8 4,929,261 - PP_4338 0.59 -3.7 4,930,019 + +0.2 4,930,019 + -4.3 4,930,019 + +0.4 4,930,019 + -2.1 4,930,019 + -0.3 4,930,020 - -2.7 4,930,020 - -4.0 4,930,020 - -0.8 4,930,020 - -2.9 4,930,112 + +0.3 4,930,113 - -2.0 4,930,128 - +0.6 4,930,185 + -0.2 4,930,186 - +1.2 4,930,249 + +0.7 4,930,334 + PP_4339 0.19 -1.4 4,930,335 - PP_4339 0.19 +1.2 4,930,450 - PP_4339 0.34 +1.4 4,930,450 - PP_4339 0.34 -0.6 4,930,701 + PP_4339 0.66 +0.5 4,930,701 + PP_4339 0.66 +1.0 4,930,701 + PP_4339 0.66 +1.5 4,930,701 + PP_4339 0.66 +1.6 4,930,701 + PP_4339 0.66 +0.9 4,930,701 + PP_4339 0.66 +1.1 4,930,702 - PP_4339 0.66 +2.9 4,930,702 - PP_4339 0.66 +2.2 4,930,702 - PP_4339 0.66 +0.5 4,930,702 - PP_4339 0.66 +0.5 4,930,702 - PP_4339 0.66 +2.0 4,930,726 + PP_4339 0.69 +0.9 4,930,726 + PP_4339 0.69 +2.7 4,931,068 + PP_4340 0.19 -3.3 4,931,068 + PP_4340 0.19 -3.9 4,931,068 + PP_4340 0.19 -0.6 4,931,233 + PP_4340 0.63 -1.1 4,931,234 - PP_4340 0.64 -1.9 4,931,383 + -3.0 4,931,383 + -3.5 4,931,384 - +0.1 4,931,416 + -0.6 4,931,444 - +1.0 4,931,444 - +1.0 4,931,605 - PP_4341 0.17 +2.4 4,931,605 - PP_4341 0.17 +2.0 4,931,607 + PP_4341 0.18 +1.9 4,931,608 - PP_4341 0.18 +1.7 4,931,812 - PP_4341 0.45 +0.8 4,931,812 - PP_4341 0.45 +2.1 4,931,814 + PP_4341 0.45 +1.4 4,931,814 + PP_4341 0.45 +2.1 4,931,814 + PP_4341 0.45 +2.1 4,931,814 + PP_4341 0.45 +1.9 4,931,815 - PP_4341 0.46 +0.6 4,931,911 + PP_4341 0.59 +2.2 4,931,928 - PP_4341 0.61 +1.3
Or see this region's nucleotide sequence