Experiment: Temperature shift 30_to_25; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0365 and PP_0366 are separated by 10 nucleotides PP_0366 and PP_0367 are separated by 173 nucleotides PP_0367 and PP_0368 are separated by 287 nucleotides
PP_0365: PP_0365 - Malonyl-[acyl-carrier protein] O-methyltransferase, at 443,009 to 443,827
_0365
PP_0366: PP_0366 - dethiobiotin synthetase, at 443,838 to 444,518
_0366
PP_0367: PP_0367 - conserved protein of unknown function, at 444,692 to 445,027
_0367
PP_0368: PP_0368 - putative Acyl-CoA dehydrogenase, at 445,315 to 447,120
_0368
Position (kb) 444
445
446 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.787 kb on - strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.826 kb on - strand at 444.043 kb on - strand, within PP_0366 at 444.043 kb on - strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.078 kb on - strand, within PP_0366 at 444.080 kb on - strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.559 kb on + strand at 444.594 kb on - strand at 444.630 kb on + strand at 444.631 kb on - strand at 444.659 kb on + strand at 444.659 kb on + strand at 444.679 kb on + strand at 444.690 kb on - strand at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.759 kb on - strand, within PP_0367 at 444.759 kb on - strand, within PP_0367 at 444.850 kb on + strand, within PP_0367 at 445.019 kb on + strand at 445.019 kb on + strand at 445.019 kb on + strand at 445.020 kb on - strand at 445.020 kb on - strand at 445.020 kb on - strand at 445.030 kb on - strand at 445.030 kb on - strand at 445.045 kb on + strand at 445.045 kb on + strand at 445.046 kb on - strand at 445.064 kb on + strand at 445.065 kb on - strand at 445.065 kb on - strand at 445.065 kb on - strand at 445.145 kb on + strand at 445.145 kb on + strand at 445.206 kb on - strand at 445.239 kb on - strand at 445.300 kb on + strand at 445.325 kb on - strand at 445.375 kb on + strand at 445.387 kb on + strand at 445.551 kb on + strand, within PP_0368 at 445.552 kb on - strand, within PP_0368 at 445.689 kb on + strand, within PP_0368 at 445.693 kb on + strand, within PP_0368 at 445.725 kb on + strand, within PP_0368 at 445.726 kb on - strand, within PP_0368 at 445.762 kb on + strand, within PP_0368 at 445.762 kb on + strand, within PP_0368 at 445.763 kb on - strand, within PP_0368 at 445.879 kb on + strand, within PP_0368 at 445.879 kb on + strand, within PP_0368 at 445.880 kb on - strand, within PP_0368 at 445.947 kb on + strand, within PP_0368
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_25; with MOPS remove 443,747 + -2.5 443,747 + -4.3 443,747 + -0.3 443,747 + -3.7 443,747 + -3.2 443,748 - -3.4 443,748 - -3.7 443,748 - -3.4 443,748 - -0.8 443,786 + -4.1 443,786 + -0.2 443,786 + -3.6 443,787 - -3.7 443,798 + -2.5 443,798 + -3.4 443,798 + -1.6 443,798 + -2.8 443,798 + +0.4 443,798 + -0.2 443,799 - -3.1 443,799 - -3.4 443,799 - -4.2 443,799 - -2.6 443,799 - -3.0 443,799 - -2.4 443,826 - -1.8 444,043 - PP_0366 0.30 -2.8 444,043 - PP_0366 0.30 -1.1 444,077 + PP_0366 0.35 -2.4 444,077 + PP_0366 0.35 -4.7 444,078 - PP_0366 0.35 -0.4 444,080 - PP_0366 0.36 -4.6 444,184 + PP_0366 0.51 -1.9 444,184 + PP_0366 0.51 -1.8 444,185 - PP_0366 0.51 -2.2 444,185 - PP_0366 0.51 -3.4 444,185 - PP_0366 0.51 -0.4 444,517 - -0.4 444,517 - -0.2 444,517 - -0.6 444,517 - -1.2 444,559 + +0.7 444,594 - +1.0 444,630 + -0.8 444,631 - +1.6 444,659 + +0.9 444,659 + -0.9 444,679 + -4.0 444,690 - +1.4 444,758 + PP_0367 0.20 +0.0 444,758 + PP_0367 0.20 +0.5 444,758 + PP_0367 0.20 -1.7 444,758 + PP_0367 0.20 -1.3 444,758 + PP_0367 0.20 +0.9 444,759 - PP_0367 0.20 +0.3 444,759 - PP_0367 0.20 +0.9 444,850 + PP_0367 0.47 -1.2 445,019 + +0.6 445,019 + -2.5 445,019 + -1.8 445,020 - +0.4 445,020 - -2.1 445,020 - +0.4 445,030 - -1.4 445,030 - -0.3 445,045 + -2.0 445,045 + +1.0 445,046 - -0.5 445,064 + -0.4 445,065 - +0.5 445,065 - +0.5 445,065 - -3.1 445,145 + -0.7 445,145 + -0.2 445,206 - +0.8 445,239 - -3.5 445,300 + +0.2 445,325 - -3.5 445,375 + -2.3 445,387 + -1.8 445,551 + PP_0368 0.13 -0.1 445,552 - PP_0368 0.13 +1.4 445,689 + PP_0368 0.21 -1.7 445,693 + PP_0368 0.21 -0.8 445,725 + PP_0368 0.23 -1.1 445,726 - PP_0368 0.23 +0.2 445,762 + PP_0368 0.25 -0.7 445,762 + PP_0368 0.25 +0.5 445,763 - PP_0368 0.25 +0.3 445,879 + PP_0368 0.31 -4.0 445,879 + PP_0368 0.31 +0.2 445,880 - PP_0368 0.31 +1.5 445,947 + PP_0368 0.35 +0.2
Or see this region's nucleotide sequence