Experiment: 3-methyl-3-butenol (pure) 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yoaA and b1809 are separated by 131 nucleotides b1809 and yoaC are separated by 421 nucleotides yoaC and yoaH are separated by 119 nucleotides yoaH and pabB are separated by 73 nucleotides
b1808: yoaA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,889,349 to 1,891,259
yoaA
b1809: b1809 - orf, hypothetical protein (VIMSS), at 1,891,391 to 1,891,735
b1809
b1810: yoaC - predicted protein (RefSeq), at 1,892,157 to 1,892,456
yoaC
b1811: yoaH - hypothetical protein (NCBI), at 1,892,576 to 1,892,755
yoaH
b1812: pabB - para-aminobenzoate synthase component I (NCBI), at 1,892,829 to 1,894,190
pabB
Position (kb)
1892
1893 Strain fitness (log2 ratio)
-1
0
1
2 at 1891.209 kb on + strand at 1891.209 kb on + strand at 1891.260 kb on + strand at 1891.403 kb on - strand at 1891.519 kb on + strand, within b1809 at 1891.533 kb on - strand, within b1809 at 1891.533 kb on - strand, within b1809 at 1891.630 kb on + strand, within b1809 at 1891.632 kb on + strand, within b1809 at 1891.632 kb on + strand, within b1809 at 1891.657 kb on - strand, within b1809 at 1891.658 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.679 kb on - strand, within b1809 at 1891.681 kb on - strand, within b1809 at 1891.730 kb on + strand at 1891.730 kb on + strand at 1891.730 kb on + strand at 1891.737 kb on - strand at 1891.738 kb on - strand at 1891.750 kb on + strand at 1891.887 kb on + strand at 1891.887 kb on + strand at 1891.892 kb on + strand at 1891.892 kb on + strand at 1891.913 kb on - strand at 1891.932 kb on - strand at 1891.951 kb on + strand at 1891.959 kb on - strand at 1891.965 kb on + strand at 1891.981 kb on - strand at 1892.003 kb on + strand at 1892.008 kb on + strand at 1892.010 kb on + strand at 1892.016 kb on - strand at 1892.016 kb on - strand at 1892.032 kb on + strand at 1892.032 kb on + strand at 1892.046 kb on + strand at 1892.046 kb on + strand at 1892.102 kb on + strand at 1892.102 kb on + strand at 1892.153 kb on + strand at 1892.166 kb on + strand at 1892.178 kb on + strand at 1892.178 kb on + strand at 1892.196 kb on + strand, within yoaC at 1892.196 kb on + strand, within yoaC at 1892.199 kb on + strand, within yoaC at 1892.201 kb on + strand, within yoaC at 1892.226 kb on - strand, within yoaC at 1892.226 kb on - strand, within yoaC at 1892.230 kb on - strand, within yoaC at 1892.290 kb on + strand, within yoaC at 1892.299 kb on + strand, within yoaC at 1892.316 kb on + strand, within yoaC at 1892.316 kb on + strand, within yoaC at 1892.333 kb on - strand, within yoaC at 1892.334 kb on - strand, within yoaC at 1892.341 kb on - strand, within yoaC at 1892.341 kb on - strand, within yoaC at 1892.382 kb on + strand, within yoaC at 1892.382 kb on + strand, within yoaC at 1892.388 kb on - strand, within yoaC at 1892.390 kb on - strand, within yoaC at 1892.421 kb on + strand, within yoaC at 1892.433 kb on - strand at 1892.437 kb on + strand at 1892.437 kb on + strand at 1892.444 kb on - strand at 1892.453 kb on - strand at 1892.498 kb on - strand at 1892.498 kb on - strand at 1892.509 kb on - strand at 1892.509 kb on - strand at 1892.567 kb on - strand at 1892.567 kb on - strand at 1892.579 kb on - strand at 1892.583 kb on + strand at 1892.636 kb on - strand, within yoaH at 1892.677 kb on - strand, within yoaH at 1892.677 kb on - strand, within yoaH at 1892.730 kb on - strand, within yoaH at 1892.746 kb on - strand at 1892.762 kb on - strand at 1892.762 kb on - strand at 1892.812 kb on + strand at 1892.843 kb on - strand at 1892.865 kb on - strand at 1892.899 kb on + strand at 1892.899 kb on + strand at 1892.933 kb on - strand at 1892.933 kb on - strand at 1892.987 kb on + strand, within pabB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (pure) 0.5 vol% remove 1,891,209 + -0.1 1,891,209 + +0.6 1,891,260 + +0.0 1,891,403 - -0.8 1,891,519 + b1809 b1809 0.37 -0.4 1,891,533 - b1809 b1809 0.41 +1.3 1,891,533 - b1809 b1809 0.41 +0.7 1,891,630 + b1809 b1809 0.69 -0.7 1,891,632 + b1809 b1809 0.70 -0.3 1,891,632 + b1809 b1809 0.70 +0.3 1,891,657 - b1809 b1809 0.77 +0.8 1,891,658 + b1809 b1809 0.77 -0.8 1,891,673 + b1809 b1809 0.82 -0.1 1,891,673 + b1809 b1809 0.82 -0.1 1,891,673 + b1809 b1809 0.82 +0.2 1,891,673 + b1809 b1809 0.82 +1.4 1,891,673 + b1809 b1809 0.82 +0.1 1,891,673 + b1809 b1809 0.82 +0.4 1,891,673 + b1809 b1809 0.82 +0.1 1,891,679 - b1809 b1809 0.83 +0.8 1,891,681 - b1809 b1809 0.84 -0.8 1,891,730 + +1.6 1,891,730 + -0.7 1,891,730 + +0.5 1,891,737 - +0.8 1,891,738 - +0.8 1,891,750 + -0.0 1,891,887 + +0.8 1,891,887 + -0.8 1,891,892 + +2.1 1,891,892 + +2.4 1,891,913 - +0.0 1,891,932 - +0.8 1,891,951 + -0.8 1,891,959 - -1.2 1,891,965 + +1.1 1,891,981 - +0.3 1,892,003 + -0.0 1,892,008 + +0.3 1,892,010 + +1.4 1,892,016 - +0.8 1,892,016 - -0.8 1,892,032 + +1.9 1,892,032 + -0.7 1,892,046 + +0.5 1,892,046 + -1.3 1,892,102 + -0.0 1,892,102 + -0.6 1,892,153 + +0.1 1,892,166 + -0.3 1,892,178 + +0.3 1,892,178 + +1.0 1,892,196 + yoaC b1810 0.13 -1.0 1,892,196 + yoaC b1810 0.13 -0.9 1,892,199 + yoaC b1810 0.14 -1.4 1,892,201 + yoaC b1810 0.15 -0.5 1,892,226 - yoaC b1810 0.23 -0.8 1,892,226 - yoaC b1810 0.23 +0.8 1,892,230 - yoaC b1810 0.24 +0.8 1,892,290 + yoaC b1810 0.44 -0.2 1,892,299 + yoaC b1810 0.47 +0.6 1,892,316 + yoaC b1810 0.53 +0.8 1,892,316 + yoaC b1810 0.53 +0.1 1,892,333 - yoaC b1810 0.59 +0.8 1,892,334 - yoaC b1810 0.59 +0.8 1,892,341 - yoaC b1810 0.61 -0.8 1,892,341 - yoaC b1810 0.61 +0.8 1,892,382 + yoaC b1810 0.75 -0.9 1,892,382 + yoaC b1810 0.75 -0.8 1,892,388 - yoaC b1810 0.77 +0.2 1,892,390 - yoaC b1810 0.78 +0.8 1,892,421 + yoaC b1810 0.88 -0.1 1,892,433 - -0.8 1,892,437 + +1.3 1,892,437 + -1.0 1,892,444 - -0.2 1,892,453 - -1.1 1,892,498 - -0.2 1,892,498 - -0.8 1,892,509 - +0.1 1,892,509 - +2.1 1,892,567 - -0.5 1,892,567 - -0.5 1,892,579 - +0.3 1,892,583 + +0.8 1,892,636 - yoaH b1811 0.33 -0.2 1,892,677 - yoaH b1811 0.56 +0.4 1,892,677 - yoaH b1811 0.56 +1.1 1,892,730 - yoaH b1811 0.86 -0.1 1,892,746 - +0.4 1,892,762 - -0.2 1,892,762 - -0.4 1,892,812 + +0.8 1,892,843 - +0.1 1,892,865 - -0.0 1,892,899 + -0.5 1,892,899 + -0.1 1,892,933 - +0.0 1,892,933 - +0.3 1,892,987 + pabB b1812 0.12 +0.5
Or see this region's nucleotide sequence