Experiment: Temperature shift 30_to_25; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0305 and PP_5442 are separated by 3 nucleotides PP_5442 and PP_0306 are separated by 73 nucleotides PP_0306 and PP_0307 are separated by 102 nucleotides
PP_0305: PP_0305 - HTH-type transcriptional regulator CdhR, at 365,734 to 366,693
_0305
PP_5442: PP_5442 - protein of unknown function, at 366,697 to 367,314
_5442
PP_0306: PP_0306 - conserved protein of unknown function, at 367,388 to 368,116
_0306
PP_0307: PP_0307 - conserved exported protein of unknown function, at 368,219 to 368,650
_0307
Position (kb) 366
367
368 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 365.711 kb on + strand at 365.711 kb on + strand at 365.851 kb on + strand, within PP_0305 at 365.851 kb on + strand, within PP_0305 at 365.868 kb on + strand, within PP_0305 at 365.869 kb on - strand, within PP_0305 at 366.151 kb on + strand, within PP_0305 at 366.521 kb on - strand, within PP_0305 at 366.619 kb on + strand at 366.619 kb on + strand at 366.619 kb on + strand at 366.620 kb on - strand at 366.620 kb on - strand at 366.685 kb on - strand at 366.728 kb on - strand at 366.857 kb on + strand, within PP_5442 at 366.857 kb on + strand, within PP_5442 at 366.864 kb on - strand, within PP_5442 at 366.949 kb on - strand, within PP_5442 at 366.954 kb on + strand, within PP_5442 at 366.955 kb on - strand, within PP_5442 at 367.044 kb on - strand, within PP_5442 at 367.044 kb on - strand, within PP_5442 at 367.044 kb on - strand, within PP_5442 at 367.076 kb on + strand, within PP_5442 at 367.076 kb on + strand, within PP_5442 at 367.076 kb on + strand, within PP_5442 at 367.076 kb on + strand, within PP_5442 at 367.077 kb on - strand, within PP_5442 at 367.077 kb on - strand, within PP_5442 at 367.089 kb on + strand, within PP_5442 at 367.089 kb on + strand, within PP_5442 at 367.090 kb on - strand, within PP_5442 at 367.095 kb on + strand, within PP_5442 at 367.095 kb on + strand, within PP_5442 at 367.095 kb on + strand, within PP_5442 at 367.095 kb on + strand, within PP_5442 at 367.095 kb on + strand, within PP_5442 at 367.096 kb on - strand, within PP_5442 at 367.138 kb on - strand, within PP_5442 at 367.193 kb on - strand, within PP_5442 at 367.193 kb on - strand, within PP_5442 at 367.216 kb on + strand, within PP_5442 at 367.216 kb on + strand, within PP_5442 at 367.216 kb on + strand, within PP_5442 at 367.216 kb on + strand, within PP_5442 at 367.216 kb on + strand, within PP_5442 at 367.216 kb on + strand, within PP_5442 at 367.217 kb on - strand, within PP_5442 at 367.217 kb on - strand, within PP_5442 at 367.236 kb on + strand, within PP_5442 at 367.236 kb on + strand, within PP_5442 at 367.236 kb on + strand, within PP_5442 at 367.237 kb on - strand, within PP_5442 at 367.305 kb on + strand at 367.306 kb on - strand at 367.393 kb on + strand at 367.560 kb on + strand, within PP_0306 at 367.884 kb on + strand, within PP_0306 at 367.885 kb on - strand, within PP_0306 at 368.025 kb on - strand, within PP_0306 at 368.096 kb on + strand at 368.096 kb on + strand at 368.097 kb on - strand at 368.146 kb on - strand at 368.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_25; with MOPS remove 365,711 + -0.3 365,711 + +0.9 365,851 + PP_0305 0.12 +0.5 365,851 + PP_0305 0.12 +1.2 365,868 + PP_0305 0.14 -1.1 365,869 - PP_0305 0.14 +1.3 366,151 + PP_0305 0.43 +0.3 366,521 - PP_0305 0.82 +0.7 366,619 + +0.9 366,619 + +0.5 366,619 + +0.7 366,620 - +0.4 366,620 - +0.5 366,685 - -0.5 366,728 - +0.3 366,857 + PP_5442 0.26 +0.5 366,857 + PP_5442 0.26 +1.2 366,864 - PP_5442 0.27 +1.4 366,949 - PP_5442 0.41 +0.4 366,954 + PP_5442 0.42 +1.3 366,955 - PP_5442 0.42 +1.0 367,044 - PP_5442 0.56 +0.8 367,044 - PP_5442 0.56 +0.7 367,044 - PP_5442 0.56 +1.3 367,076 + PP_5442 0.61 -1.5 367,076 + PP_5442 0.61 +1.1 367,076 + PP_5442 0.61 +1.2 367,076 + PP_5442 0.61 +1.4 367,077 - PP_5442 0.61 -2.3 367,077 - PP_5442 0.61 -0.5 367,089 + PP_5442 0.63 -0.9 367,089 + PP_5442 0.63 -0.2 367,090 - PP_5442 0.64 -2.8 367,095 + PP_5442 0.64 -1.3 367,095 + PP_5442 0.64 -2.3 367,095 + PP_5442 0.64 -1.6 367,095 + PP_5442 0.64 +0.3 367,095 + PP_5442 0.64 -2.1 367,096 - PP_5442 0.65 +0.0 367,138 - PP_5442 0.71 +0.1 367,193 - PP_5442 0.80 +2.1 367,193 - PP_5442 0.80 +0.6 367,216 + PP_5442 0.84 -1.6 367,216 + PP_5442 0.84 +0.9 367,216 + PP_5442 0.84 -1.7 367,216 + PP_5442 0.84 -0.4 367,216 + PP_5442 0.84 -1.0 367,216 + PP_5442 0.84 -1.9 367,217 - PP_5442 0.84 +1.8 367,217 - PP_5442 0.84 +0.8 367,236 + PP_5442 0.87 -0.9 367,236 + PP_5442 0.87 -2.6 367,236 + PP_5442 0.87 +0.3 367,237 - PP_5442 0.87 +0.8 367,305 + +1.1 367,306 - -0.5 367,393 + +0.6 367,560 + PP_0306 0.24 +0.0 367,884 + PP_0306 0.68 +0.7 367,885 - PP_0306 0.68 -1.2 368,025 - PP_0306 0.87 +1.7 368,096 + -0.5 368,096 + +0.8 368,097 - +0.6 368,146 - +1.0 368,157 - +0.6
Or see this region's nucleotide sequence