Experiment: Temperature shift 30_to_25; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3758 and PP_3759 overlap by 7 nucleotides PP_3759 and PP_3760 overlap by 4 nucleotides PP_3760 and PP_3761 overlap by 4 nucleotides
PP_3758: PP_3758 - Response regulator/sensor histidine kinase, at 4,286,509 to 4,287,735
_3758
PP_3759: PP_3759 - Methylesterase, CheB-like, at 4,287,729 to 4,288,304
_3759
PP_3760: PP_3760 - putative methyltransferase, at 4,288,301 to 4,289,122
_3760
PP_3761: PP_3761 - Sensor histidine kinase/response regulator, at 4,289,119 to 4,292,586
_3761
Position (kb)
4288
4289
4290 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4287.437 kb on + strand, within PP_3758 at 4287.453 kb on - strand, within PP_3758 at 4287.731 kb on - strand at 4287.742 kb on + strand at 4287.743 kb on - strand at 4287.782 kb on + strand at 4287.782 kb on + strand at 4287.788 kb on + strand, within PP_3759 at 4287.788 kb on + strand, within PP_3759 at 4287.789 kb on - strand, within PP_3759 at 4287.889 kb on + strand, within PP_3759 at 4287.890 kb on - strand, within PP_3759 at 4287.890 kb on - strand, within PP_3759 at 4287.956 kb on - strand, within PP_3759 at 4288.081 kb on + strand, within PP_3759 at 4288.081 kb on + strand, within PP_3759 at 4288.081 kb on + strand, within PP_3759 at 4288.081 kb on + strand, within PP_3759 at 4288.081 kb on + strand, within PP_3759 at 4288.081 kb on + strand, within PP_3759 at 4288.082 kb on - strand, within PP_3759 at 4288.082 kb on - strand, within PP_3759 at 4288.082 kb on - strand, within PP_3759 at 4288.082 kb on - strand, within PP_3759 at 4288.082 kb on - strand, within PP_3759 at 4288.082 kb on - strand, within PP_3759 at 4288.126 kb on - strand, within PP_3759 at 4288.309 kb on + strand at 4288.309 kb on + strand at 4288.384 kb on + strand, within PP_3760 at 4288.596 kb on + strand, within PP_3760 at 4288.596 kb on + strand, within PP_3760 at 4288.596 kb on + strand, within PP_3760 at 4288.596 kb on + strand, within PP_3760 at 4288.596 kb on + strand, within PP_3760 at 4288.599 kb on + strand, within PP_3760 at 4288.671 kb on + strand, within PP_3760 at 4288.723 kb on - strand, within PP_3760 at 4288.776 kb on + strand, within PP_3760 at 4288.776 kb on + strand, within PP_3760 at 4288.777 kb on - strand, within PP_3760 at 4288.914 kb on + strand, within PP_3760 at 4289.037 kb on + strand, within PP_3760 at 4289.052 kb on + strand at 4289.058 kb on + strand at 4289.058 kb on + strand at 4289.059 kb on - strand at 4289.059 kb on - strand at 4289.067 kb on + strand at 4289.068 kb on - strand at 4289.196 kb on - strand at 4289.196 kb on - strand at 4289.460 kb on + strand at 4289.460 kb on + strand at 4289.461 kb on - strand at 4289.461 kb on - strand at 4289.463 kb on + strand at 4289.464 kb on - strand at 4289.464 kb on - strand at 4289.464 kb on - strand at 4289.648 kb on + strand, within PP_3761 at 4289.649 kb on - strand, within PP_3761 at 4289.696 kb on + strand, within PP_3761 at 4289.697 kb on - strand, within PP_3761 at 4289.802 kb on - strand, within PP_3761 at 4289.802 kb on - strand, within PP_3761 at 4289.802 kb on - strand, within PP_3761 at 4289.802 kb on - strand, within PP_3761 at 4290.011 kb on + strand, within PP_3761
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_25; with MOPS remove 4,287,437 + PP_3758 0.76 -1.7 4,287,453 - PP_3758 0.77 +0.4 4,287,731 - +0.6 4,287,742 + +0.3 4,287,743 - -0.1 4,287,782 + +1.0 4,287,782 + +0.9 4,287,788 + PP_3759 0.10 +0.8 4,287,788 + PP_3759 0.10 +0.8 4,287,789 - PP_3759 0.10 -1.4 4,287,889 + PP_3759 0.28 -1.7 4,287,890 - PP_3759 0.28 +0.9 4,287,890 - PP_3759 0.28 +1.8 4,287,956 - PP_3759 0.39 +1.3 4,288,081 + PP_3759 0.61 -0.3 4,288,081 + PP_3759 0.61 -0.1 4,288,081 + PP_3759 0.61 -0.3 4,288,081 + PP_3759 0.61 -0.3 4,288,081 + PP_3759 0.61 +0.6 4,288,081 + PP_3759 0.61 +0.0 4,288,082 - PP_3759 0.61 +0.0 4,288,082 - PP_3759 0.61 +1.1 4,288,082 - PP_3759 0.61 +1.9 4,288,082 - PP_3759 0.61 -0.1 4,288,082 - PP_3759 0.61 +0.6 4,288,082 - PP_3759 0.61 -0.8 4,288,126 - PP_3759 0.69 -0.8 4,288,309 + +0.4 4,288,309 + -0.3 4,288,384 + PP_3760 0.10 -1.2 4,288,596 + PP_3760 0.36 -0.2 4,288,596 + PP_3760 0.36 +0.0 4,288,596 + PP_3760 0.36 -3.1 4,288,596 + PP_3760 0.36 -0.3 4,288,596 + PP_3760 0.36 +0.0 4,288,599 + PP_3760 0.36 +0.5 4,288,671 + PP_3760 0.45 -1.0 4,288,723 - PP_3760 0.51 +1.8 4,288,776 + PP_3760 0.58 -0.9 4,288,776 + PP_3760 0.58 -0.1 4,288,777 - PP_3760 0.58 -1.6 4,288,914 + PP_3760 0.75 -0.1 4,289,037 + PP_3760 0.90 +0.5 4,289,052 + +0.5 4,289,058 + +0.3 4,289,058 + +0.5 4,289,059 - -0.8 4,289,059 - -1.0 4,289,067 + +0.4 4,289,068 - +0.2 4,289,196 - +0.3 4,289,196 - +2.8 4,289,460 + +0.9 4,289,460 + +0.5 4,289,461 - +1.2 4,289,461 - +0.8 4,289,463 + +0.6 4,289,464 - +1.1 4,289,464 - +0.1 4,289,464 - +0.9 4,289,648 + PP_3761 0.15 +0.4 4,289,649 - PP_3761 0.15 -0.2 4,289,696 + PP_3761 0.17 +0.8 4,289,697 - PP_3761 0.17 +1.3 4,289,802 - PP_3761 0.20 -0.1 4,289,802 - PP_3761 0.20 +1.2 4,289,802 - PP_3761 0.20 -0.5 4,289,802 - PP_3761 0.20 +0.6 4,290,011 + PP_3761 0.26 +0.8
Or see this region's nucleotide sequence