Experiment: 3-methyl-2-butenol (commercial) 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cdd and sanA are separated by 149 nucleotides sanA and yeiS are separated by 2 nucleotides yeiS and yeiT are separated by 193 nucleotides
b2143: cdd - cytidine deaminase (NCBI), at 2,229,866 to 2,230,750
cdd
b2144: sanA - hypothetical protein (NCBI), at 2,230,900 to 2,231,619
sanA
b2145: yeiS - predicted inner membrane protein (NCBI), at 2,231,622 to 2,231,861
yeiS
b2146: yeiT - predicted oxidoreductase (NCBI), at 2,232,055 to 2,233,293
yeiT
Position (kb)
2230
2231
2232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2229.972 kb on - strand, within cdd at 2229.972 kb on - strand, within cdd at 2229.984 kb on - strand, within cdd at 2230.246 kb on + strand, within cdd at 2230.255 kb on - strand, within cdd at 2230.398 kb on - strand, within cdd at 2230.398 kb on - strand, within cdd at 2230.624 kb on - strand, within cdd at 2230.685 kb on + strand at 2230.685 kb on + strand at 2230.776 kb on + strand at 2230.898 kb on + strand at 2230.933 kb on - strand at 2230.938 kb on - strand at 2230.998 kb on + strand, within sanA at 2230.998 kb on + strand, within sanA at 2231.014 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.025 kb on + strand, within sanA at 2231.025 kb on + strand, within sanA at 2231.026 kb on + strand, within sanA at 2231.031 kb on + strand, within sanA at 2231.031 kb on + strand, within sanA at 2231.031 kb on - strand, within sanA at 2231.032 kb on + strand, within sanA at 2231.032 kb on + strand, within sanA at 2231.032 kb on + strand, within sanA at 2231.033 kb on - strand, within sanA at 2231.033 kb on - strand, within sanA at 2231.054 kb on - strand, within sanA at 2231.055 kb on - strand, within sanA at 2231.055 kb on - strand, within sanA at 2231.091 kb on + strand, within sanA at 2231.118 kb on + strand, within sanA at 2231.119 kb on + strand, within sanA at 2231.127 kb on - strand, within sanA at 2231.142 kb on + strand, within sanA at 2231.163 kb on - strand, within sanA at 2231.191 kb on - strand, within sanA at 2231.193 kb on + strand, within sanA at 2231.212 kb on + strand, within sanA at 2231.212 kb on + strand, within sanA at 2231.222 kb on + strand, within sanA at 2231.224 kb on + strand, within sanA at 2231.224 kb on + strand, within sanA at 2231.227 kb on + strand, within sanA at 2231.230 kb on - strand, within sanA at 2231.230 kb on - strand, within sanA at 2231.232 kb on - strand, within sanA at 2231.234 kb on + strand, within sanA at 2231.238 kb on + strand, within sanA at 2231.238 kb on + strand, within sanA at 2231.243 kb on + strand, within sanA at 2231.243 kb on + strand, within sanA at 2231.266 kb on - strand, within sanA at 2231.267 kb on + strand, within sanA at 2231.267 kb on + strand, within sanA at 2231.267 kb on + strand, within sanA at 2231.270 kb on + strand, within sanA at 2231.279 kb on + strand, within sanA at 2231.279 kb on + strand, within sanA at 2231.290 kb on - strand, within sanA at 2231.338 kb on + strand, within sanA at 2231.348 kb on - strand, within sanA at 2231.363 kb on - strand, within sanA at 2231.366 kb on - strand, within sanA at 2231.389 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.396 kb on + strand, within sanA at 2231.399 kb on + strand, within sanA at 2231.402 kb on - strand, within sanA at 2231.402 kb on - strand, within sanA at 2231.402 kb on - strand, within sanA at 2231.407 kb on - strand, within sanA at 2231.407 kb on - strand, within sanA at 2231.412 kb on + strand, within sanA at 2231.415 kb on + strand, within sanA at 2231.419 kb on + strand, within sanA at 2231.424 kb on + strand, within sanA at 2231.424 kb on + strand, within sanA at 2231.427 kb on + strand, within sanA at 2231.459 kb on + strand, within sanA at 2231.469 kb on + strand, within sanA at 2231.512 kb on - strand, within sanA at 2231.514 kb on + strand, within sanA at 2231.517 kb on + strand, within sanA at 2231.524 kb on + strand, within sanA at 2231.525 kb on - strand, within sanA at 2231.537 kb on + strand, within sanA at 2231.543 kb on - strand, within sanA at 2231.571 kb on + strand at 2231.636 kb on + strand at 2231.675 kb on - strand, within yeiS at 2231.691 kb on + strand, within yeiS at 2231.691 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.817 kb on + strand, within yeiS at 2231.836 kb on + strand, within yeiS at 2231.981 kb on - strand at 2231.992 kb on + strand at 2231.992 kb on + strand at 2232.005 kb on - strand at 2232.016 kb on + strand at 2232.046 kb on - strand at 2232.056 kb on - strand at 2232.056 kb on - strand at 2232.139 kb on - strand at 2232.139 kb on - strand at 2232.140 kb on - strand at 2232.140 kb on - strand at 2232.149 kb on + strand at 2232.151 kb on + strand at 2232.151 kb on + strand at 2232.151 kb on + strand at 2232.151 kb on + strand at 2232.153 kb on + strand at 2232.155 kb on + strand at 2232.155 kb on + strand at 2232.155 kb on + strand at 2232.159 kb on - strand at 2232.159 kb on - strand at 2232.159 kb on - strand at 2232.161 kb on - strand at 2232.162 kb on + strand at 2232.167 kb on + strand at 2232.170 kb on - strand at 2232.171 kb on + strand at 2232.172 kb on + strand at 2232.205 kb on + strand, within yeiT at 2232.213 kb on - strand, within yeiT at 2232.272 kb on + strand, within yeiT at 2232.278 kb on + strand, within yeiT at 2232.280 kb on - strand, within yeiT at 2232.280 kb on - strand, within yeiT at 2232.311 kb on + strand, within yeiT at 2232.311 kb on + strand, within yeiT at 2232.326 kb on + strand, within yeiT at 2232.326 kb on + strand, within yeiT at 2232.326 kb on + strand, within yeiT at 2232.333 kb on - strand, within yeiT at 2232.333 kb on - strand, within yeiT at 2232.346 kb on + strand, within yeiT at 2232.473 kb on + strand, within yeiT at 2232.575 kb on + strand, within yeiT
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol (commercial) 0.5 vol% remove 2,229,972 - cdd b2143 0.12 +0.2 2,229,972 - cdd b2143 0.12 +0.4 2,229,984 - cdd b2143 0.13 +0.4 2,230,246 + cdd b2143 0.43 -0.3 2,230,255 - cdd b2143 0.44 +0.3 2,230,398 - cdd b2143 0.60 -0.1 2,230,398 - cdd b2143 0.60 +0.2 2,230,624 - cdd b2143 0.86 +0.3 2,230,685 + +0.2 2,230,685 + -0.8 2,230,776 + -0.9 2,230,898 + +1.1 2,230,933 - -0.6 2,230,938 - -0.5 2,230,998 + sanA b2144 0.14 +0.7 2,230,998 + sanA b2144 0.14 +0.3 2,231,014 + sanA b2144 0.16 +0.6 2,231,023 + sanA b2144 0.17 +0.2 2,231,023 + sanA b2144 0.17 -0.2 2,231,023 + sanA b2144 0.17 +0.5 2,231,023 + sanA b2144 0.17 -0.3 2,231,023 + sanA b2144 0.17 +0.7 2,231,025 + sanA b2144 0.17 -0.1 2,231,025 + sanA b2144 0.17 +0.1 2,231,026 + sanA b2144 0.17 -0.7 2,231,031 + sanA b2144 0.18 -0.7 2,231,031 + sanA b2144 0.18 -0.2 2,231,031 - sanA b2144 0.18 -0.5 2,231,032 + sanA b2144 0.18 -0.4 2,231,032 + sanA b2144 0.18 +0.5 2,231,032 + sanA b2144 0.18 +0.1 2,231,033 - sanA b2144 0.18 +0.3 2,231,033 - sanA b2144 0.18 -0.4 2,231,054 - sanA b2144 0.21 +1.1 2,231,055 - sanA b2144 0.22 +1.1 2,231,055 - sanA b2144 0.22 +1.1 2,231,091 + sanA b2144 0.27 -0.1 2,231,118 + sanA b2144 0.30 -0.3 2,231,119 + sanA b2144 0.30 -0.6 2,231,127 - sanA b2144 0.32 +1.1 2,231,142 + sanA b2144 0.34 +0.1 2,231,163 - sanA b2144 0.37 -0.5 2,231,191 - sanA b2144 0.40 +1.1 2,231,193 + sanA b2144 0.41 -0.3 2,231,212 + sanA b2144 0.43 -1.5 2,231,212 + sanA b2144 0.43 -1.1 2,231,222 + sanA b2144 0.45 -0.5 2,231,224 + sanA b2144 0.45 +0.1 2,231,224 + sanA b2144 0.45 -0.3 2,231,227 + sanA b2144 0.45 -0.4 2,231,230 - sanA b2144 0.46 +0.1 2,231,230 - sanA b2144 0.46 +2.4 2,231,232 - sanA b2144 0.46 +0.1 2,231,234 + sanA b2144 0.46 -0.8 2,231,238 + sanA b2144 0.47 -0.1 2,231,238 + sanA b2144 0.47 +0.8 2,231,243 + sanA b2144 0.48 +1.2 2,231,243 + sanA b2144 0.48 -0.3 2,231,266 - sanA b2144 0.51 +2.1 2,231,267 + sanA b2144 0.51 +1.4 2,231,267 + sanA b2144 0.51 -1.7 2,231,267 + sanA b2144 0.51 +1.0 2,231,270 + sanA b2144 0.51 +1.1 2,231,279 + sanA b2144 0.53 +1.1 2,231,279 + sanA b2144 0.53 +0.7 2,231,290 - sanA b2144 0.54 +1.1 2,231,338 + sanA b2144 0.61 -0.0 2,231,348 - sanA b2144 0.62 -0.5 2,231,363 - sanA b2144 0.64 -0.0 2,231,366 - sanA b2144 0.65 +2.1 2,231,389 + sanA b2144 0.68 +0.8 2,231,394 + sanA b2144 0.69 +0.7 2,231,394 + sanA b2144 0.69 +0.1 2,231,394 + sanA b2144 0.69 +1.8 2,231,394 + sanA b2144 0.69 +1.1 2,231,394 + sanA b2144 0.69 +0.6 2,231,394 + sanA b2144 0.69 +1.1 2,231,394 + sanA b2144 0.69 -0.3 2,231,396 + sanA b2144 0.69 +0.1 2,231,399 + sanA b2144 0.69 +2.1 2,231,402 - sanA b2144 0.70 +0.1 2,231,402 - sanA b2144 0.70 +1.1 2,231,402 - sanA b2144 0.70 +0.5 2,231,407 - sanA b2144 0.70 +2.6 2,231,407 - sanA b2144 0.70 -0.4 2,231,412 + sanA b2144 0.71 -0.8 2,231,415 + sanA b2144 0.72 +0.8 2,231,419 + sanA b2144 0.72 +0.2 2,231,424 + sanA b2144 0.73 +0.1 2,231,424 + sanA b2144 0.73 +0.2 2,231,427 + sanA b2144 0.73 +0.8 2,231,459 + sanA b2144 0.78 -1.3 2,231,469 + sanA b2144 0.79 -1.1 2,231,512 - sanA b2144 0.85 +2.1 2,231,514 + sanA b2144 0.85 +0.3 2,231,517 + sanA b2144 0.86 +0.3 2,231,524 + sanA b2144 0.87 +0.5 2,231,525 - sanA b2144 0.87 +1.1 2,231,537 + sanA b2144 0.88 +1.3 2,231,543 - sanA b2144 0.89 +1.1 2,231,571 + -0.3 2,231,636 + +0.6 2,231,675 - yeiS b2145 0.22 -0.5 2,231,691 + yeiS b2145 0.29 -2.4 2,231,691 + yeiS b2145 0.29 -0.4 2,231,748 + yeiS b2145 0.53 -0.1 2,231,748 + yeiS b2145 0.53 +0.1 2,231,748 + yeiS b2145 0.53 +0.0 2,231,748 + yeiS b2145 0.53 -0.9 2,231,748 + yeiS b2145 0.53 +0.1 2,231,817 + yeiS b2145 0.81 +0.1 2,231,836 + yeiS b2145 0.89 +1.2 2,231,981 - -0.0 2,231,992 + +1.1 2,231,992 + +1.1 2,232,005 - -1.5 2,232,016 + +2.6 2,232,046 - +1.6 2,232,056 - +0.2 2,232,056 - +0.3 2,232,139 - +0.9 2,232,139 - -0.7 2,232,140 - +1.1 2,232,140 - +1.6 2,232,149 + +2.1 2,232,151 + +0.1 2,232,151 + +1.1 2,232,151 + -1.9 2,232,151 + +1.1 2,232,153 + -0.5 2,232,155 + +0.3 2,232,155 + +0.1 2,232,155 + +1.1 2,232,159 - -0.3 2,232,159 - -1.3 2,232,159 - +0.1 2,232,161 - -0.2 2,232,162 + -0.5 2,232,167 + +1.1 2,232,170 - +0.6 2,232,171 + -1.3 2,232,172 + +0.1 2,232,205 + yeiT b2146 0.12 -0.0 2,232,213 - yeiT b2146 0.13 +0.6 2,232,272 + yeiT b2146 0.18 -1.3 2,232,278 + yeiT b2146 0.18 +0.2 2,232,280 - yeiT b2146 0.18 +1.1 2,232,280 - yeiT b2146 0.18 -0.3 2,232,311 + yeiT b2146 0.21 +1.4 2,232,311 + yeiT b2146 0.21 +0.3 2,232,326 + yeiT b2146 0.22 +0.4 2,232,326 + yeiT b2146 0.22 -0.5 2,232,326 + yeiT b2146 0.22 +0.1 2,232,333 - yeiT b2146 0.22 +2.1 2,232,333 - yeiT b2146 0.22 +0.6 2,232,346 + yeiT b2146 0.23 -1.3 2,232,473 + yeiT b2146 0.34 -0.4 2,232,575 + yeiT b2146 0.42 -0.3
Or see this region's nucleotide sequence