Experiment: Temperature shift 30_to_25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3361 and PP_3362 are separated by 143 nucleotides PP_3362 and PP_3363 are separated by 40 nucleotides PP_3363 and PP_3364 are separated by 226 nucleotides
PP_3361: PP_3361 - putative aminotransferase, at 3,800,869 to 3,803,916
_3361
PP_3362: PP_3362 - Transcriptional regulator, AsnC family, at 3,804,060 to 3,804,500
_3362
PP_3363: PP_3363 - putative thymidylate kinase, at 3,804,541 to 3,805,158
_3363
PP_3364: PP_3364 - Response regulator, at 3,805,385 to 3,807,928
_3364
Position (kb)
3804
3805
3806 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3803.776 kb on + strand at 3803.776 kb on + strand at 3803.776 kb on + strand at 3803.777 kb on - strand at 3803.796 kb on - strand at 3803.916 kb on + strand at 3803.916 kb on + strand at 3803.917 kb on - strand at 3803.917 kb on - strand at 3803.917 kb on - strand at 3803.952 kb on + strand at 3803.953 kb on - strand at 3803.988 kb on + strand at 3803.989 kb on - strand at 3804.100 kb on + strand at 3804.148 kb on + strand, within PP_3362 at 3804.149 kb on - strand, within PP_3362 at 3804.239 kb on - strand, within PP_3362 at 3804.254 kb on - strand, within PP_3362 at 3804.452 kb on + strand, within PP_3362 at 3804.452 kb on + strand, within PP_3362 at 3804.452 kb on + strand, within PP_3362 at 3804.453 kb on - strand, within PP_3362 at 3804.501 kb on + strand at 3804.502 kb on - strand at 3804.502 kb on - strand at 3804.516 kb on + strand at 3804.699 kb on + strand, within PP_3363 at 3804.907 kb on + strand, within PP_3363 at 3804.907 kb on + strand, within PP_3363 at 3804.907 kb on + strand, within PP_3363 at 3804.908 kb on - strand, within PP_3363 at 3804.908 kb on - strand, within PP_3363 at 3804.908 kb on - strand at 3805.166 kb on + strand at 3805.190 kb on - strand at 3805.205 kb on - strand at 3805.235 kb on - strand at 3805.243 kb on - strand at 3805.326 kb on - strand at 3805.391 kb on + strand at 3805.391 kb on + strand at 3805.391 kb on + strand at 3805.391 kb on + strand at 3805.391 kb on + strand at 3805.392 kb on - strand at 3805.404 kb on + strand at 3805.404 kb on + strand at 3805.539 kb on + strand at 3805.539 kb on + strand at 3805.539 kb on + strand at 3805.540 kb on - strand at 3805.550 kb on - strand at 3805.597 kb on + strand at 3805.598 kb on - strand at 3805.598 kb on - strand at 3805.598 kb on - strand at 3805.703 kb on + strand, within PP_3364 at 3805.703 kb on + strand, within PP_3364 at 3805.703 kb on + strand, within PP_3364 at 3805.703 kb on + strand, within PP_3364 at 3805.877 kb on + strand, within PP_3364 at 3805.878 kb on - strand, within PP_3364 at 3805.878 kb on - strand, within PP_3364 at 3805.884 kb on + strand, within PP_3364 at 3805.884 kb on + strand, within PP_3364 at 3805.884 kb on + strand, within PP_3364 at 3805.885 kb on - strand, within PP_3364 at 3805.885 kb on - strand, within PP_3364 at 3805.956 kb on - strand, within PP_3364 at 3805.998 kb on - strand, within PP_3364 at 3806.041 kb on + strand, within PP_3364 at 3806.041 kb on + strand, within PP_3364 at 3806.042 kb on - strand, within PP_3364 at 3806.096 kb on + strand, within PP_3364 at 3806.097 kb on - strand, within PP_3364 at 3806.097 kb on - strand, within PP_3364 at 3806.135 kb on + strand, within PP_3364 at 3806.135 kb on + strand, within PP_3364 at 3806.136 kb on - strand, within PP_3364
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_25 remove 3,803,776 + +1.0 3,803,776 + +0.3 3,803,776 + +0.3 3,803,777 - -0.4 3,803,796 - -0.8 3,803,916 + +0.5 3,803,916 + -1.6 3,803,917 - +3.1 3,803,917 - -0.3 3,803,917 - +0.7 3,803,952 + -1.2 3,803,953 - -1.0 3,803,988 + +0.3 3,803,989 - -0.2 3,804,100 + -2.1 3,804,148 + PP_3362 0.20 -0.2 3,804,149 - PP_3362 0.20 +1.0 3,804,239 - PP_3362 0.41 -0.1 3,804,254 - PP_3362 0.44 -0.7 3,804,452 + PP_3362 0.89 -0.1 3,804,452 + PP_3362 0.89 +0.3 3,804,452 + PP_3362 0.89 +0.7 3,804,453 - PP_3362 0.89 +0.3 3,804,501 + -0.4 3,804,502 - -1.1 3,804,502 - +0.4 3,804,516 + -4.2 3,804,699 + PP_3363 0.26 -2.2 3,804,907 + PP_3363 0.59 +0.4 3,804,907 + PP_3363 0.59 +0.0 3,804,907 + PP_3363 0.59 -1.7 3,804,908 - PP_3363 0.59 -2.8 3,804,908 - PP_3363 0.59 -0.8 3,804,908 - +1.0 3,805,166 + +0.9 3,805,190 - -1.2 3,805,205 - +0.8 3,805,235 - +1.4 3,805,243 - +0.4 3,805,326 - -0.1 3,805,391 + -0.1 3,805,391 + +0.5 3,805,391 + +0.6 3,805,391 + +0.4 3,805,391 + -1.5 3,805,392 - -0.7 3,805,404 + +0.1 3,805,404 + +0.0 3,805,539 + -0.9 3,805,539 + +1.8 3,805,539 + -0.4 3,805,540 - +0.4 3,805,550 - -1.7 3,805,597 + -0.1 3,805,598 - +1.2 3,805,598 - -1.0 3,805,598 - +0.8 3,805,703 + PP_3364 0.12 +0.2 3,805,703 + PP_3364 0.12 +0.2 3,805,703 + PP_3364 0.12 +0.8 3,805,703 + PP_3364 0.12 -1.8 3,805,877 + PP_3364 0.19 +1.1 3,805,878 - PP_3364 0.19 +0.9 3,805,878 - PP_3364 0.19 +0.0 3,805,884 + PP_3364 0.20 +0.3 3,805,884 + PP_3364 0.20 -0.3 3,805,884 + PP_3364 0.20 +0.1 3,805,885 - PP_3364 0.20 +0.8 3,805,885 - PP_3364 0.20 -0.6 3,805,956 - PP_3364 0.22 +1.9 3,805,998 - PP_3364 0.24 +1.4 3,806,041 + PP_3364 0.26 +1.1 3,806,041 + PP_3364 0.26 +0.9 3,806,042 - PP_3364 0.26 +1.8 3,806,096 + PP_3364 0.28 -0.1 3,806,097 - PP_3364 0.28 +0.3 3,806,097 - PP_3364 0.28 +0.6 3,806,135 + PP_3364 0.29 -0.2 3,806,135 + PP_3364 0.29 +1.0 3,806,136 - PP_3364 0.30 +0.6
Or see this region's nucleotide sequence