Experiment: Temperature shift 30_to_25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0004 and PP_0005 are separated by 630 nucleotides PP_0005 and PP_0006 are separated by 88 nucleotides
PP_0004: PP_0004 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme, at 2,486 to 4,381
_0004
PP_0005: PP_0005 - GTPase, at 5,012 to 6,382
_0005
PP_0006: PP_0006 - probable membrane protein insertase, at 6,471 to 8,153
_0006
Position (kb)
5
6
7 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4.416 kb on - strand at 4.428 kb on - strand at 4.428 kb on - strand at 4.428 kb on - strand at 4.544 kb on + strand at 4.550 kb on + strand at 4.550 kb on + strand at 4.551 kb on - strand at 4.590 kb on + strand at 4.603 kb on + strand at 4.603 kb on + strand at 4.604 kb on - strand at 4.614 kb on + strand at 4.615 kb on - strand at 4.672 kb on + strand at 4.673 kb on - strand at 4.723 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.979 kb on - strand at 5.014 kb on - strand at 6.397 kb on - strand at 6.415 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.465 kb on - strand at 6.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_25 remove 4,416 - -0.1 4,428 - -0.0 4,428 - +1.3 4,428 - -1.3 4,544 + +1.6 4,550 + -1.3 4,550 + +0.2 4,551 - +0.1 4,590 + -3.7 4,603 + -0.8 4,603 + +0.2 4,604 - +0.7 4,614 + +0.0 4,615 - -0.4 4,672 + -0.7 4,673 - -0.3 4,723 + +1.0 4,853 + +0.0 4,853 + +0.6 4,853 + -1.6 4,853 + -0.3 4,853 + -1.9 4,853 + -0.3 4,853 + -0.7 4,854 - -1.8 4,854 - +0.1 4,854 - -0.2 4,854 - -0.8 4,854 - -1.6 4,854 - +0.5 4,854 - +1.5 4,878 + -0.1 4,878 + -1.6 4,878 + +1.6 4,878 + -0.1 4,878 + +0.3 4,878 + -0.4 4,879 - -1.0 4,879 - +1.2 4,879 - -0.3 4,879 - +0.1 4,879 - +0.6 4,979 - -0.3 5,014 - -0.6 6,397 - -0.9 6,415 - -0.4 6,462 - -0.4 6,462 - -0.0 6,462 - -1.9 6,462 - +0.2 6,462 - -3.3 6,462 - +1.0 6,465 - -0.6 6,492 - -0.7
Or see this region's nucleotide sequence