Experiment: 3-methyl-3-butenol (commercial) 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfjR and ypjK are separated by 40 nucleotides ypjK and b2636 overlap by 1 nucleotides b2636 and yfjT are separated by 23 nucleotides yfjT and yfjU are separated by 386 nucleotides yfjU and yfjV are separated by 12 nucleotides
b2634: yfjR - CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 2,767,725 to 2,768,426
yfjR
b2635: ypjK - CP4-57 prophage; predicted inner membrane protein (NCBI), at 2,768,467 to 2,768,703
ypjK
b2636: b2636 - orf, hypothetical protein (VIMSS), at 2,768,703 to 2,769,146
b2636
b2637: yfjT - CP4-57 prophage; predicted protein (NCBI), at 2,769,170 to 2,769,637
yfjT
b2638: yfjU - CP4-57 prophage; conserved protein (RefSeq), at 2,770,024 to 2,770,176
yfjU
b2641: yfjV - orf, hypothetical protein (VIMSS), at 2,770,189 to 2,771,204
yfjV
Position (kb)
2769
2770 Strain fitness (log2 ratio)
-2
-1
0
1 at 2768.215 kb on + strand, within yfjR at 2768.258 kb on - strand, within yfjR at 2768.258 kb on - strand, within yfjR at 2768.388 kb on - strand at 2768.429 kb on - strand at 2768.429 kb on - strand at 2768.437 kb on + strand at 2768.445 kb on - strand at 2768.460 kb on - strand at 2768.470 kb on - strand at 2768.582 kb on - strand, within ypjK at 2768.582 kb on - strand, within ypjK at 2768.582 kb on - strand, within ypjK at 2768.791 kb on - strand, within b2636 at 2768.810 kb on + strand, within b2636 at 2768.843 kb on - strand, within b2636 at 2768.845 kb on - strand, within b2636 at 2768.919 kb on - strand, within b2636 at 2768.941 kb on - strand, within b2636 at 2768.941 kb on - strand, within b2636 at 2769.051 kb on - strand, within b2636 at 2769.064 kb on - strand, within b2636 at 2769.064 kb on - strand, within b2636 at 2769.099 kb on + strand, within b2636 at 2769.099 kb on + strand, within b2636 at 2769.118 kb on - strand at 2769.242 kb on - strand, within yfjT at 2769.288 kb on - strand, within yfjT at 2769.528 kb on - strand, within yfjT at 2769.528 kb on - strand, within yfjT at 2769.650 kb on + strand at 2769.650 kb on + strand at 2769.658 kb on + strand at 2769.737 kb on - strand at 2769.737 kb on - strand at 2769.822 kb on - strand at 2769.822 kb on - strand at 2769.945 kb on - strand at 2770.051 kb on + strand, within yfjU at 2770.051 kb on + strand, within yfjU at 2770.070 kb on - strand, within yfjU at 2770.124 kb on - strand, within yfjU at 2770.124 kb on - strand, within yfjU at 2770.127 kb on - strand, within yfjU at 2770.176 kb on + strand at 2770.194 kb on + strand at 2770.194 kb on + strand at 2770.239 kb on + strand at 2770.247 kb on - strand at 2770.357 kb on + strand, within yfjV at 2770.357 kb on + strand, within yfjV at 2770.422 kb on + strand, within yfjV at 2770.433 kb on + strand, within yfjV at 2770.433 kb on + strand, within yfjV at 2770.570 kb on + strand, within yfjV at 2770.570 kb on + strand, within yfjV at 2770.594 kb on + strand, within yfjV at 2770.594 kb on + strand, within yfjV at 2770.602 kb on - strand, within yfjV at 2770.609 kb on + strand, within yfjV at 2770.609 kb on + strand, within yfjV
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (commercial) 0.5 vol% remove 2,768,215 + yfjR b2634 0.70 -2.6 2,768,258 - yfjR b2634 0.76 -0.6 2,768,258 - yfjR b2634 0.76 +0.3 2,768,388 - +0.6 2,768,429 - -0.2 2,768,429 - +1.0 2,768,437 + +0.5 2,768,445 - -0.3 2,768,460 - +0.1 2,768,470 - +1.4 2,768,582 - ypjK b2635 0.49 -1.1 2,768,582 - ypjK b2635 0.49 +0.2 2,768,582 - ypjK b2635 0.49 +0.7 2,768,791 - b2636 b2636 0.20 -0.4 2,768,810 + b2636 b2636 0.24 +0.1 2,768,843 - b2636 b2636 0.32 +0.0 2,768,845 - b2636 b2636 0.32 -0.1 2,768,919 - b2636 b2636 0.49 +0.8 2,768,941 - b2636 b2636 0.54 -0.3 2,768,941 - b2636 b2636 0.54 -0.1 2,769,051 - b2636 b2636 0.78 +0.0 2,769,064 - b2636 b2636 0.81 -0.4 2,769,064 - b2636 b2636 0.81 -0.6 2,769,099 + b2636 b2636 0.89 +0.7 2,769,099 + b2636 b2636 0.89 -0.4 2,769,118 - -0.8 2,769,242 - yfjT b2637 0.15 +1.1 2,769,288 - yfjT b2637 0.25 +0.6 2,769,528 - yfjT b2637 0.76 -0.6 2,769,528 - yfjT b2637 0.76 +0.2 2,769,650 + +0.6 2,769,650 + -0.1 2,769,658 + +0.0 2,769,737 - +0.2 2,769,737 - -0.5 2,769,822 - +0.6 2,769,822 - -0.1 2,769,945 - -0.6 2,770,051 + yfjU b2638 0.18 -0.7 2,770,051 + yfjU b2638 0.18 -0.9 2,770,070 - yfjU b2638 0.30 -0.1 2,770,124 - yfjU b2638 0.65 -0.7 2,770,124 - yfjU b2638 0.65 +0.3 2,770,127 - yfjU b2638 0.67 -0.1 2,770,176 + +0.7 2,770,194 + -0.6 2,770,194 + +0.2 2,770,239 + -0.0 2,770,247 - -0.7 2,770,357 + yfjV b2641 0.17 -0.3 2,770,357 + yfjV b2641 0.17 +0.7 2,770,422 + yfjV b2641 0.23 -0.4 2,770,433 + yfjV b2641 0.24 -0.5 2,770,433 + yfjV b2641 0.24 +0.2 2,770,570 + yfjV b2641 0.38 -0.5 2,770,570 + yfjV b2641 0.38 +0.0 2,770,594 + yfjV b2641 0.40 +0.3 2,770,594 + yfjV b2641 0.40 -0.5 2,770,602 - yfjV b2641 0.41 +0.1 2,770,609 + yfjV b2641 0.41 +0.4 2,770,609 + yfjV b2641 0.41 -0.2
Or see this region's nucleotide sequence