Strain Fitness in Escherichia coli BW25113 around b2490

Experiment: 3-methyl-3-butenol (commercial) 0.5 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-3-butenol (commercial) 0.5 vol%
2,608,658	-	hyfH	b2488	0.86	+0.5
2,608,658	-	hyfH	b2488	0.86	+0.5
2,608,733	-				+0.7
2,608,733	-				+0.8
2,608,757	-				+0.1
2,609,013	+	hyfI	b2489	0.38	+0.7
2,609,013	+	hyfI	b2489	0.38	+0.5
2,609,095	-	hyfI	b2489	0.48	-0.7
2,609,097	+	hyfI	b2489	0.49	-0.6
2,609,290	-	hyfI	b2489	0.74	-0.2
2,609,329	+	hyfI	b2489	0.79	-0.2
2,609,329	+	hyfI	b2489	0.79	-0.1
2,609,393	-	hyfI	b2489	0.88	-0.4
2,609,393	-	hyfI	b2489	0.88	-0.3
2,609,480	+				+0.1
2,609,482	+				-0.5
2,609,490	-				-0.2
2,609,490	-				+0.1
2,609,576	-	b2490	b2490	0.23	+0.9
2,609,576	-	b2490	b2490	0.23	+0.6
2,609,595	-	b2490	b2490	0.28	+0.6
2,609,671	-	b2490	b2490	0.46	-0.1
2,610,075	-				-0.3
2,610,080	-				-0.0
2,610,149	-	hyfR	b2491	0.11	+0.2
2,610,261	+	hyfR	b2491	0.17	+0.4
2,610,284	-	hyfR	b2491	0.18	-0.2
2,610,286	-	hyfR	b2491	0.18	+0.3
2,610,406	+	hyfR	b2491	0.24	+0.1
2,610,411	+	hyfR	b2491	0.24	+0.4
2,610,555	+	hyfR	b2491	0.31	-1.1
2,610,555	+	hyfR	b2491	0.31	+1.9
2,610,733	-	hyfR	b2491	0.40	-0.1
2,610,768	-	hyfR	b2491	0.42	-1.6
2,610,768	-	hyfR	b2491	0.42	+0.3
2,610,852	-	hyfR	b2491	0.46	+0.8
2,610,852	-	hyfR	b2491	0.46	+0.1