Experiment: 3-methyl-3-butenol (commercial) 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and moaB are separated by 21 nucleotides moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides
b0781: moaA - molybdenum cofactor biosynthesis protein A (NCBI), at 816,267 to 817,256
moaA
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
Position (kb) 817
818 Strain fitness (log2 ratio) -2
-1
0
1 at 816.333 kb on - strand at 816.363 kb on + strand at 816.363 kb on + strand at 816.378 kb on - strand, within moaA at 816.609 kb on - strand, within moaA at 816.624 kb on + strand, within moaA at 816.680 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.782 kb on - strand, within moaA at 816.817 kb on + strand, within moaA at 816.912 kb on + strand, within moaA at 817.009 kb on - strand, within moaA at 817.009 kb on - strand, within moaA at 817.049 kb on - strand, within moaA at 817.085 kb on - strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.280 kb on - strand at 817.280 kb on - strand at 817.355 kb on - strand, within moaB at 817.485 kb on + strand, within moaB at 817.485 kb on + strand, within moaB at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (commercial) 0.5 vol% remove 816,333 - -0.8 816,363 + -0.1 816,363 + +1.2 816,378 - moaA b0781 0.11 +1.2 816,609 - moaA b0781 0.35 -2.1 816,624 + moaA b0781 0.36 -0.0 816,680 + moaA b0781 0.42 -0.0 816,746 + moaA b0781 0.48 -0.1 816,746 + moaA b0781 0.48 +0.2 816,782 - moaA b0781 0.52 -0.4 816,817 + moaA b0781 0.56 +0.4 816,912 + moaA b0781 0.65 +0.5 817,009 - moaA b0781 0.75 +0.1 817,009 - moaA b0781 0.75 +0.3 817,049 - moaA b0781 0.79 +0.2 817,085 - moaA b0781 0.83 -0.9 817,105 + moaA b0781 0.85 -0.5 817,105 + moaA b0781 0.85 +0.4 817,105 + moaA b0781 0.85 +0.4 817,280 - -1.0 817,280 - +0.1 817,355 - moaB b0782 0.15 +0.8 817,485 + moaB b0782 0.40 +0.4 817,485 + moaB b0782 0.40 -0.4 817,818 - +0.2 817,818 - +0.2 817,852 - moaC b0783 0.12 +0.0 817,852 - moaC b0783 0.12 +0.6 817,872 - moaC b0783 0.16 -1.8 817,912 + moaC b0783 0.24 +1.2 817,912 + moaC b0783 0.24 +0.7 818,006 - moaC b0783 0.44 +1.0 818,012 - moaC b0783 0.45 +0.5 818,167 - moaC b0783 0.77 -1.0 818,211 + moaC b0783 0.86 -0.2 818,219 - moaC b0783 0.88 -0.7 818,219 - moaC b0783 0.88 -0.0 818,252 + +0.8 818,252 + +1.4 818,369 + moaD b0784 0.40 -0.4 818,455 + moaD b0784 0.75 +1.2 818,455 + moaD b0784 0.75 +0.2 818,566 - moaE b0785 0.11 +0.5 818,566 - moaE b0785 0.11 -0.1 818,566 - moaE b0785 0.11 +1.3 818,677 - moaE b0785 0.35 -0.1 818,687 - moaE b0785 0.37 +1.7 818,764 - moaE b0785 0.54 +0.4
Or see this region's nucleotide sequence