Experiment: 3-methyl-3-butenol (commercial) 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaY and ybaZ are separated by 30 nucleotides ybaZ and ffs are separated by 76 nucleotides ffs and ybaA are separated by 110 nucleotides ybaA and ylaB are separated by 41 nucleotides
b0453: ybaY - glycoprotein/polysaccharide metabolism (VIMSS), at 474,603 to 475,175
ybaY
b0454: ybaZ - predicted methyltransferase (NCBI), at 475,206 to 475,595
ybaZ
b0455: ffs - 4.5S RNA (NCBI), at 475,672 to 475,785
ffs
b0456: ybaA - hypothetical protein (NCBI), at 475,896 to 476,249
ybaA
b0457: ylaB - orf, hypothetical protein (VIMSS), at 476,291 to 477,841
ylaB
Position (kb) 475
476
477 Strain fitness (log2 ratio) -2
-1
0
1 at 474.912 kb on + strand, within ybaY at 474.956 kb on - strand, within ybaY at 474.956 kb on - strand, within ybaY at 474.966 kb on - strand, within ybaY at 474.989 kb on + strand, within ybaY at 475.003 kb on - strand, within ybaY at 475.003 kb on - strand, within ybaY at 475.074 kb on + strand, within ybaY at 475.133 kb on + strand at 475.153 kb on + strand at 475.188 kb on - strand at 475.189 kb on + strand at 475.210 kb on - strand at 475.293 kb on + strand, within ybaZ at 475.293 kb on - strand, within ybaZ at 475.365 kb on - strand, within ybaZ at 475.413 kb on - strand, within ybaZ at 475.413 kb on - strand, within ybaZ at 475.436 kb on - strand, within ybaZ at 475.444 kb on - strand, within ybaZ at 475.444 kb on - strand, within ybaZ at 475.463 kb on - strand, within ybaZ at 475.483 kb on - strand, within ybaZ at 475.964 kb on + strand, within ybaA at 476.028 kb on - strand, within ybaA at 476.028 kb on - strand, within ybaA at 476.167 kb on + strand, within ybaA at 476.195 kb on + strand, within ybaA at 476.201 kb on - strand, within ybaA at 476.349 kb on + strand at 476.349 kb on + strand at 476.353 kb on + strand at 476.383 kb on - strand at 476.446 kb on - strand, within ylaB at 476.521 kb on - strand, within ylaB at 476.547 kb on - strand, within ylaB at 476.547 kb on - strand, within ylaB at 476.573 kb on - strand, within ylaB at 476.594 kb on + strand, within ylaB at 476.662 kb on + strand, within ylaB at 476.662 kb on + strand, within ylaB at 476.662 kb on - strand, within ylaB at 476.662 kb on - strand, within ylaB at 476.670 kb on - strand, within ylaB at 476.677 kb on + strand, within ylaB at 476.685 kb on - strand, within ylaB at 476.685 kb on - strand, within ylaB at 476.690 kb on - strand, within ylaB at 476.690 kb on - strand, within ylaB at 476.696 kb on - strand, within ylaB at 476.752 kb on + strand, within ylaB at 476.752 kb on + strand at 476.774 kb on + strand, within ylaB at 476.775 kb on - strand, within ylaB at 476.801 kb on + strand, within ylaB at 476.806 kb on - strand, within ylaB at 476.806 kb on - strand, within ylaB at 476.839 kb on - strand, within ylaB at 476.839 kb on - strand, within ylaB at 476.862 kb on - strand, within ylaB at 476.904 kb on - strand, within ylaB at 476.910 kb on + strand, within ylaB at 476.910 kb on + strand, within ylaB at 476.911 kb on + strand, within ylaB at 476.981 kb on - strand, within ylaB at 477.034 kb on + strand, within ylaB at 477.034 kb on + strand, within ylaB at 477.056 kb on - strand, within ylaB at 477.067 kb on - strand, within ylaB at 477.067 kb on - strand, within ylaB at 477.069 kb on + strand, within ylaB at 477.069 kb on + strand, within ylaB at 477.089 kb on - strand, within ylaB at 477.089 kb on - strand, within ylaB at 477.089 kb on - strand, within ylaB at 477.161 kb on - strand, within ylaB at 477.161 kb on - strand, within ylaB at 477.188 kb on + strand, within ylaB at 477.196 kb on - strand, within ylaB at 477.196 kb on - strand, within ylaB at 477.196 kb on - strand, within ylaB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (commercial) 0.5 vol% remove 474,912 + ybaY b0453 0.54 -0.5 474,956 - ybaY b0453 0.62 -0.7 474,956 - ybaY b0453 0.62 +1.1 474,966 - ybaY b0453 0.63 +0.1 474,989 + ybaY b0453 0.67 -0.1 475,003 - ybaY b0453 0.70 +0.9 475,003 - ybaY b0453 0.70 +0.0 475,074 + ybaY b0453 0.82 +0.2 475,133 + +0.8 475,153 + +0.3 475,188 - +0.9 475,189 + +0.5 475,210 - -0.2 475,293 + ybaZ b0454 0.22 +0.2 475,293 - ybaZ b0454 0.22 -0.5 475,365 - ybaZ b0454 0.41 -0.4 475,413 - ybaZ b0454 0.53 -0.1 475,413 - ybaZ b0454 0.53 +0.2 475,436 - ybaZ b0454 0.59 +0.4 475,444 - ybaZ b0454 0.61 +1.1 475,444 - ybaZ b0454 0.61 +0.2 475,463 - ybaZ b0454 0.66 +0.0 475,483 - ybaZ b0454 0.71 +0.1 475,964 + ybaA b0456 0.19 +0.8 476,028 - ybaA b0456 0.37 +1.1 476,028 - ybaA b0456 0.37 +1.7 476,167 + ybaA b0456 0.77 -0.3 476,195 + ybaA b0456 0.84 -0.0 476,201 - ybaA b0456 0.86 +0.3 476,349 + +0.9 476,349 + +0.2 476,353 + -0.2 476,383 - -0.2 476,446 - ylaB b0457 0.10 +0.0 476,521 - ylaB b0457 0.15 -0.4 476,547 - ylaB b0457 0.17 +0.2 476,547 - ylaB b0457 0.17 +0.6 476,573 - ylaB b0457 0.18 -0.6 476,594 + ylaB b0457 0.20 -0.8 476,662 + ylaB b0457 0.24 +0.4 476,662 + ylaB b0457 0.24 +1.4 476,662 - ylaB b0457 0.24 +1.7 476,662 - ylaB b0457 0.24 +1.1 476,670 - ylaB b0457 0.24 +0.4 476,677 + ylaB b0457 0.25 +0.3 476,685 - ylaB b0457 0.25 +0.4 476,685 - ylaB b0457 0.25 +0.3 476,690 - ylaB b0457 0.26 -0.4 476,690 - ylaB b0457 0.26 +0.7 476,696 - ylaB b0457 0.26 +1.2 476,752 + ylaB b0457 0.30 +0.9 476,752 + -1.3 476,774 + ylaB b0457 0.31 -0.5 476,775 - ylaB b0457 0.31 +0.1 476,801 + ylaB b0457 0.33 -0.4 476,806 - ylaB b0457 0.33 -0.6 476,806 - ylaB b0457 0.33 -0.5 476,839 - ylaB b0457 0.35 +0.1 476,839 - ylaB b0457 0.35 +0.4 476,862 - ylaB b0457 0.37 +0.7 476,904 - ylaB b0457 0.40 -0.6 476,910 + ylaB b0457 0.40 -2.1 476,910 + ylaB b0457 0.40 +0.2 476,911 + ylaB b0457 0.40 +0.6 476,981 - ylaB b0457 0.44 +0.4 477,034 + ylaB b0457 0.48 -0.1 477,034 + ylaB b0457 0.48 -0.3 477,056 - ylaB b0457 0.49 -1.8 477,067 - ylaB b0457 0.50 -0.6 477,067 - ylaB b0457 0.50 -0.9 477,069 + ylaB b0457 0.50 +0.1 477,069 + ylaB b0457 0.50 -0.2 477,089 - ylaB b0457 0.51 -1.1 477,089 - ylaB b0457 0.51 -0.3 477,089 - ylaB b0457 0.51 +0.0 477,161 - ylaB b0457 0.56 -0.0 477,161 - ylaB b0457 0.56 +0.7 477,188 + ylaB b0457 0.58 +0.2 477,196 - ylaB b0457 0.58 -0.4 477,196 - ylaB b0457 0.58 -0.0 477,196 - ylaB b0457 0.58 +0.2
Or see this region's nucleotide sequence