Experiment: 3-methyl-3-butenol (commercial) 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpB and prpC are separated by 439 nucleotides prpC and prpD are separated by 33 nucleotides
b0331: prpB - 2-methylisocitrate lyase (NCBI), at 347,906 to 348,796
prpB
b0333: prpC - 2-methylcitrate synthase (NCBI), at 349,236 to 350,405
prpC
b0334: prpD - 2-methylcitrate dehydratase (NCBI), at 350,439 to 351,890
prpD
Position (kb) 349
350
351 Strain fitness (log2 ratio) -1
0
1 at 348.294 kb on - strand, within prpB at 348.493 kb on - strand, within prpB at 348.511 kb on + strand, within prpB at 348.554 kb on + strand, within prpB at 348.653 kb on + strand, within prpB at 348.655 kb on + strand, within prpB at 348.655 kb on + strand, within prpB at 348.702 kb on + strand, within prpB at 348.702 kb on + strand, within prpB at 348.774 kb on - strand at 348.858 kb on + strand at 348.858 kb on + strand at 348.883 kb on - strand at 348.932 kb on + strand at 348.932 kb on + strand at 348.953 kb on + strand at 348.958 kb on + strand at 349.167 kb on + strand at 349.167 kb on + strand at 349.232 kb on + strand at 349.328 kb on + strand at 349.336 kb on - strand at 349.427 kb on + strand, within prpC at 349.502 kb on + strand, within prpC at 349.502 kb on + strand, within prpC at 349.642 kb on - strand, within prpC at 349.691 kb on + strand, within prpC at 349.691 kb on + strand, within prpC at 349.744 kb on - strand, within prpC at 349.797 kb on - strand, within prpC at 349.895 kb on - strand, within prpC at 350.020 kb on - strand, within prpC at 350.068 kb on - strand, within prpC at 350.069 kb on - strand, within prpC at 350.069 kb on - strand, within prpC at 350.142 kb on + strand, within prpC at 350.187 kb on + strand, within prpC at 350.327 kb on - strand at 350.327 kb on - strand at 350.395 kb on + strand at 350.398 kb on - strand at 350.550 kb on - strand at 350.550 kb on - strand at 350.626 kb on - strand, within prpD at 350.639 kb on - strand, within prpD at 350.639 kb on - strand, within prpD at 350.771 kb on - strand, within prpD at 351.073 kb on + strand, within prpD at 351.080 kb on + strand, within prpD at 351.080 kb on + strand, within prpD at 351.236 kb on + strand, within prpD
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (commercial) 0.5 vol% remove 348,294 - prpB b0331 0.44 +1.2 348,493 - prpB b0331 0.66 +0.5 348,511 + prpB b0331 0.68 -0.3 348,554 + prpB b0331 0.73 -0.3 348,653 + prpB b0331 0.84 -0.2 348,655 + prpB b0331 0.84 +0.4 348,655 + prpB b0331 0.84 +0.2 348,702 + prpB b0331 0.89 +0.6 348,702 + prpB b0331 0.89 +0.1 348,774 - +0.7 348,858 + +1.0 348,858 + -1.0 348,883 - +1.5 348,932 + +1.0 348,932 + +0.4 348,953 + -0.1 348,958 + +0.4 349,167 + +1.1 349,167 + -0.0 349,232 + -0.3 349,328 + -0.5 349,336 - -0.0 349,427 + prpC b0333 0.16 -0.1 349,502 + prpC b0333 0.23 +0.2 349,502 + prpC b0333 0.23 +0.3 349,642 - prpC b0333 0.35 -0.5 349,691 + prpC b0333 0.39 +0.0 349,691 + prpC b0333 0.39 -0.5 349,744 - prpC b0333 0.43 +0.6 349,797 - prpC b0333 0.48 -0.1 349,895 - prpC b0333 0.56 +0.3 350,020 - prpC b0333 0.67 +0.3 350,068 - prpC b0333 0.71 +0.0 350,069 - prpC b0333 0.71 +0.1 350,069 - prpC b0333 0.71 +0.2 350,142 + prpC b0333 0.77 -0.2 350,187 + prpC b0333 0.81 -0.2 350,327 - -0.1 350,327 - +1.3 350,395 + -0.6 350,398 - +0.3 350,550 - +1.5 350,550 - +0.1 350,626 - prpD b0334 0.13 -0.2 350,639 - prpD b0334 0.14 -0.4 350,639 - prpD b0334 0.14 -0.5 350,771 - prpD b0334 0.23 -0.2 351,073 + prpD b0334 0.44 +0.5 351,080 + prpD b0334 0.44 +0.6 351,080 + prpD b0334 0.44 +0.1 351,236 + prpD b0334 0.55 +0.5
Or see this region's nucleotide sequence