Experiment: Butyrolactam 3 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
Position (kb) 810
811
812 Strain fitness (log2 ratio) -1
0
1 at 809.993 kb on + strand, within bioF at 809.993 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.359 kb on + strand, within bioF at 810.362 kb on - strand, within bioF at 810.393 kb on - strand, within bioF at 810.414 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.673 kb on + strand at 810.673 kb on - strand at 810.729 kb on + strand at 810.791 kb on + strand at 810.833 kb on + strand, within bioC at 810.971 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.366 kb on - strand, within bioC at 811.423 kb on + strand, within bioC at 811.423 kb on + strand, within bioC at 811.431 kb on - strand at 811.446 kb on - strand at 811.446 kb on - strand at 811.464 kb on - strand at 811.559 kb on + strand at 811.672 kb on + strand, within bioD at 811.720 kb on - strand, within bioD at 811.757 kb on + strand, within bioD at 812.000 kb on + strand, within bioD at 812.005 kb on - strand, within bioD at 812.005 kb on - strand, within bioD at 812.051 kb on + strand, within bioD at 812.051 kb on + strand, within bioD at 812.061 kb on - strand, within bioD at 812.295 kb on - strand at 812.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Butyrolactam 3 vol% remove 809,993 + bioF b0776 0.34 -0.1 809,993 + bioF b0776 0.34 -0.6 810,237 + bioF b0776 0.55 -0.0 810,237 + bioF b0776 0.55 -0.3 810,359 + bioF b0776 0.65 +0.1 810,362 - bioF b0776 0.66 -0.1 810,393 - bioF b0776 0.68 -0.3 810,414 + bioF b0776 0.70 +0.1 810,492 + bioF b0776 0.77 -0.8 810,492 + bioF b0776 0.77 -0.5 810,673 + -0.3 810,673 - +0.4 810,729 + +0.0 810,791 + -0.2 810,833 + bioC b0777 0.12 -0.8 810,971 + bioC b0777 0.30 -0.3 811,122 + bioC b0777 0.50 -0.7 811,122 + bioC b0777 0.50 +0.2 811,122 + bioC b0777 0.50 -0.9 811,122 + bioC b0777 0.50 +0.2 811,326 + bioC b0777 0.77 +0.7 811,326 + bioC b0777 0.77 +0.2 811,326 + bioC b0777 0.77 +0.8 811,366 - bioC b0777 0.82 +0.8 811,423 + bioC b0777 0.90 +1.1 811,423 + bioC b0777 0.90 -0.9 811,431 - +0.3 811,446 - -0.3 811,446 - -0.3 811,464 - -0.3 811,559 + -0.4 811,672 + bioD b0778 0.26 +1.4 811,720 - bioD b0778 0.33 +0.3 811,757 + bioD b0778 0.39 +0.4 812,000 + bioD b0778 0.75 +0.5 812,005 - bioD b0778 0.76 +0.4 812,005 - bioD b0778 0.76 -0.4 812,051 + bioD b0778 0.82 -1.7 812,051 + bioD b0778 0.82 -0.8 812,061 - bioD b0778 0.84 +0.0 812,295 - +0.1 812,353 - -1.3
Or see this region's nucleotide sequence