Experiment: pinene 0.3125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menB and yfbB are separated by 14 nucleotides yfbB and menD overlap by 4 nucleotides menD and menF are separated by 88 nucleotides
b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841
menB
b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614
yfbB
b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281
menD
b2265: menF - isochorismate hydroxymutase 2, menaquinone biosynthesis (VIMSS), at 2,377,370 to 2,378,665
menF
Position (kb)
2375
2376
2377
2378 Strain fitness (log2 ratio)
-2
-1
0
1 at 2374.673 kb on - strand, within menB at 2374.831 kb on + strand at 2374.831 kb on + strand at 2374.885 kb on + strand at 2374.893 kb on - strand at 2375.223 kb on - strand, within yfbB at 2375.307 kb on - strand, within yfbB at 2375.363 kb on - strand, within yfbB at 2375.570 kb on + strand at 2375.570 kb on + strand at 2375.616 kb on - strand at 2375.616 kb on - strand at 2375.795 kb on - strand, within menD at 2376.112 kb on - strand, within menD at 2376.143 kb on - strand, within menD at 2376.143 kb on - strand, within menD at 2376.359 kb on + strand, within menD at 2376.359 kb on + strand, within menD at 2376.515 kb on + strand, within menD at 2376.554 kb on - strand, within menD at 2376.585 kb on + strand, within menD at 2376.585 kb on + strand, within menD at 2376.605 kb on + strand, within menD at 2376.790 kb on + strand, within menD at 2376.798 kb on - strand, within menD at 2376.798 kb on - strand, within menD at 2377.105 kb on + strand, within menD at 2377.105 kb on + strand, within menD at 2377.151 kb on - strand at 2377.217 kb on - strand at 2377.217 kb on - strand at 2377.232 kb on - strand at 2377.294 kb on + strand at 2377.312 kb on + strand at 2377.320 kb on - strand at 2377.320 kb on - strand at 2377.320 kb on - strand at 2377.398 kb on + strand at 2377.451 kb on + strand at 2377.531 kb on + strand, within menF at 2377.590 kb on - strand, within menF at 2377.590 kb on - strand, within menF at 2377.662 kb on - strand, within menF at 2377.692 kb on + strand, within menF at 2377.692 kb on + strand, within menF at 2377.692 kb on + strand, within menF at 2377.814 kb on - strand, within menF at 2377.851 kb on + strand, within menF at 2377.901 kb on - strand, within menF at 2378.222 kb on + strand, within menF at 2378.224 kb on + strand, within menF at 2378.261 kb on + strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF
Per-strain Table
Position Strand Gene LocusTag Fraction pinene 0.3125 vol% remove 2,374,673 - menB b2262 0.80 -1.4 2,374,831 + -1.3 2,374,831 + -1.1 2,374,885 + -0.2 2,374,893 - -0.7 2,375,223 - yfbB b2263 0.48 -0.3 2,375,307 - yfbB b2263 0.59 -1.4 2,375,363 - yfbB b2263 0.67 -0.8 2,375,570 + -0.4 2,375,570 + -0.0 2,375,616 - +1.1 2,375,616 - +0.1 2,375,795 - menD b2264 0.11 -0.9 2,376,112 - menD b2264 0.30 -0.8 2,376,143 - menD b2264 0.32 -1.7 2,376,143 - menD b2264 0.32 -1.1 2,376,359 + menD b2264 0.45 -1.4 2,376,359 + menD b2264 0.45 -1.0 2,376,515 + menD b2264 0.54 -1.2 2,376,554 - menD b2264 0.56 -0.6 2,376,585 + menD b2264 0.58 -0.7 2,376,585 + menD b2264 0.58 -0.0 2,376,605 + menD b2264 0.59 -1.5 2,376,790 + menD b2264 0.71 -1.0 2,376,798 - menD b2264 0.71 -0.9 2,376,798 - menD b2264 0.71 -0.6 2,377,105 + menD b2264 0.89 -0.8 2,377,105 + menD b2264 0.89 -1.2 2,377,151 - -1.0 2,377,217 - -0.7 2,377,217 - -0.3 2,377,232 - -1.4 2,377,294 + +0.2 2,377,312 + +0.2 2,377,320 - +0.6 2,377,320 - +0.2 2,377,320 - +0.4 2,377,398 + +0.2 2,377,451 + -0.2 2,377,531 + menF b2265 0.12 +0.2 2,377,590 - menF b2265 0.17 +0.5 2,377,590 - menF b2265 0.17 +1.1 2,377,662 - menF b2265 0.23 +1.3 2,377,692 + menF b2265 0.25 -0.7 2,377,692 + menF b2265 0.25 -0.8 2,377,692 + menF b2265 0.25 +0.2 2,377,814 - menF b2265 0.34 +1.2 2,377,851 + menF b2265 0.37 -0.2 2,377,901 - menF b2265 0.41 +1.1 2,378,222 + menF b2265 0.66 +0.0 2,378,224 + menF b2265 0.66 +0.3 2,378,261 + menF b2265 0.69 -0.2 2,378,269 - menF b2265 0.69 -2.3 2,378,269 - menF b2265 0.69 +0.6 2,378,269 - menF b2265 0.69 +0.3 2,378,269 - menF b2265 0.69 +0.9 2,378,269 - menF b2265 0.69 +0.4
Or see this region's nucleotide sequence