Experiment: pinene 0.3125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpA and glpB overlap by 11 nucleotides glpB and glpC overlap by 4 nucleotides
b2241: glpA - sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI), at 2,350,669 to 2,352,297
glpA
b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546
glpB
b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733
glpC
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-1
0
1 at 2351.298 kb on + strand, within glpA at 2351.353 kb on + strand, within glpA at 2351.353 kb on + strand, within glpA at 2351.392 kb on - strand, within glpA at 2351.608 kb on - strand, within glpA at 2351.686 kb on - strand, within glpA at 2351.686 kb on - strand, within glpA at 2351.773 kb on + strand, within glpA at 2351.773 kb on + strand, within glpA at 2351.792 kb on + strand, within glpA at 2351.792 kb on + strand, within glpA at 2351.801 kb on + strand, within glpA at 2351.801 kb on + strand, within glpA at 2351.802 kb on + strand, within glpA at 2351.802 kb on + strand, within glpA at 2351.809 kb on - strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.826 kb on + strand, within glpA at 2351.947 kb on + strand, within glpA at 2351.947 kb on + strand, within glpA at 2351.955 kb on - strand, within glpA at 2351.955 kb on - strand, within glpA at 2352.006 kb on - strand, within glpA at 2352.059 kb on + strand, within glpA at 2352.059 kb on + strand, within glpA at 2352.067 kb on - strand, within glpA at 2352.074 kb on - strand, within glpA at 2352.104 kb on + strand, within glpA at 2352.104 kb on + strand, within glpA at 2352.122 kb on + strand, within glpA at 2352.122 kb on + strand, within glpA at 2352.265 kb on + strand at 2352.300 kb on - strand at 2352.326 kb on + strand at 2352.347 kb on + strand at 2352.369 kb on + strand at 2352.440 kb on - strand, within glpB at 2352.447 kb on + strand, within glpB at 2352.461 kb on - strand, within glpB at 2352.463 kb on + strand, within glpB at 2352.463 kb on + strand, within glpB at 2352.506 kb on + strand, within glpB at 2352.506 kb on + strand, within glpB at 2352.547 kb on + strand, within glpB at 2352.631 kb on + strand, within glpB at 2352.650 kb on + strand, within glpB at 2352.650 kb on + strand, within glpB at 2352.787 kb on - strand, within glpB at 2353.281 kb on + strand, within glpB at 2353.291 kb on + strand, within glpB at 2353.291 kb on + strand, within glpB at 2353.326 kb on - strand, within glpB at 2353.326 kb on - strand, within glpB at 2353.379 kb on - strand, within glpB at 2353.400 kb on + strand, within glpB at 2353.400 kb on + strand, within glpB at 2353.436 kb on + strand at 2353.436 kb on + strand at 2353.446 kb on + strand at 2353.575 kb on - strand at 2353.575 kb on - strand at 2353.577 kb on + strand at 2353.577 kb on + strand at 2353.615 kb on - strand at 2353.684 kb on + strand, within glpC at 2353.686 kb on + strand, within glpC at 2353.696 kb on - strand, within glpC at 2353.696 kb on - strand, within glpC at 2353.767 kb on + strand, within glpC at 2353.812 kb on + strand, within glpC at 2353.853 kb on + strand, within glpC at 2353.853 kb on + strand, within glpC at 2353.871 kb on - strand, within glpC at 2354.115 kb on - strand, within glpC at 2354.125 kb on - strand, within glpC at 2354.125 kb on - strand, within glpC at 2354.169 kb on + strand, within glpC at 2354.278 kb on - strand, within glpC at 2354.438 kb on - strand, within glpC at 2354.534 kb on + strand, within glpC at 2354.542 kb on - strand, within glpC
Per-strain Table
Position Strand Gene LocusTag Fraction pinene 0.3125 vol% remove 2,351,298 + glpA b2241 0.39 +1.1 2,351,353 + glpA b2241 0.42 -0.5 2,351,353 + glpA b2241 0.42 +0.4 2,351,392 - glpA b2241 0.44 +0.1 2,351,608 - glpA b2241 0.58 -1.3 2,351,686 - glpA b2241 0.62 +0.1 2,351,686 - glpA b2241 0.62 -0.3 2,351,773 + glpA b2241 0.68 -0.5 2,351,773 + glpA b2241 0.68 +0.1 2,351,792 + glpA b2241 0.69 +0.0 2,351,792 + glpA b2241 0.69 -0.5 2,351,801 + glpA b2241 0.69 -0.3 2,351,801 + glpA b2241 0.69 +0.5 2,351,802 + glpA b2241 0.70 +0.4 2,351,802 + glpA b2241 0.70 +0.2 2,351,809 - glpA b2241 0.70 +0.4 2,351,816 + glpA b2241 0.70 -1.2 2,351,816 + glpA b2241 0.70 +1.4 2,351,816 + glpA b2241 0.70 +0.2 2,351,816 + glpA b2241 0.70 +0.1 2,351,826 + glpA b2241 0.71 -0.3 2,351,947 + glpA b2241 0.78 -0.8 2,351,947 + glpA b2241 0.78 +0.8 2,351,955 - glpA b2241 0.79 -0.2 2,351,955 - glpA b2241 0.79 +0.2 2,352,006 - glpA b2241 0.82 -0.0 2,352,059 + glpA b2241 0.85 +0.6 2,352,059 + glpA b2241 0.85 +0.9 2,352,067 - glpA b2241 0.86 -0.6 2,352,074 - glpA b2241 0.86 +1.4 2,352,104 + glpA b2241 0.88 +0.3 2,352,104 + glpA b2241 0.88 -0.3 2,352,122 + glpA b2241 0.89 -1.3 2,352,122 + glpA b2241 0.89 -0.4 2,352,265 + +1.0 2,352,300 - -0.7 2,352,326 + -0.1 2,352,347 + -0.3 2,352,369 + +0.5 2,352,440 - glpB b2242 0.12 -0.4 2,352,447 + glpB b2242 0.13 -0.7 2,352,461 - glpB b2242 0.14 -0.4 2,352,463 + glpB b2242 0.14 -0.1 2,352,463 + glpB b2242 0.14 +0.1 2,352,506 + glpB b2242 0.17 +0.6 2,352,506 + glpB b2242 0.17 -0.1 2,352,547 + glpB b2242 0.21 -1.0 2,352,631 + glpB b2242 0.27 -0.0 2,352,650 + glpB b2242 0.29 -0.2 2,352,650 + glpB b2242 0.29 -0.1 2,352,787 - glpB b2242 0.40 +0.0 2,353,281 + glpB b2242 0.79 -0.7 2,353,291 + glpB b2242 0.80 -0.0 2,353,291 + glpB b2242 0.80 +0.2 2,353,326 - glpB b2242 0.82 -1.2 2,353,326 - glpB b2242 0.82 +1.5 2,353,379 - glpB b2242 0.87 -1.4 2,353,400 + glpB b2242 0.88 +0.3 2,353,400 + glpB b2242 0.88 -0.1 2,353,436 + -0.4 2,353,436 + -0.3 2,353,446 + +1.1 2,353,575 - +0.8 2,353,575 - +0.0 2,353,577 + -0.0 2,353,577 + -0.3 2,353,615 - +0.5 2,353,684 + glpC b2243 0.12 -0.1 2,353,686 + glpC b2243 0.12 -0.0 2,353,696 - glpC b2243 0.13 +1.4 2,353,696 - glpC b2243 0.13 -0.0 2,353,767 + glpC b2243 0.19 +0.2 2,353,812 + glpC b2243 0.23 +1.0 2,353,853 + glpC b2243 0.26 +0.1 2,353,853 + glpC b2243 0.26 -0.2 2,353,871 - glpC b2243 0.28 -0.4 2,354,115 - glpC b2243 0.48 +0.5 2,354,125 - glpC b2243 0.49 -0.0 2,354,125 - glpC b2243 0.49 -0.6 2,354,169 + glpC b2243 0.53 +0.1 2,354,278 - glpC b2243 0.62 +0.2 2,354,438 - glpC b2243 0.75 -0.2 2,354,534 + glpC b2243 0.83 -0.7 2,354,542 - glpC b2243 0.84 -0.9
Or see this region's nucleotide sequence