Experiment: pinene 0.3125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylbE and ylbF are separated by 10 nucleotides ylbF and ybcF overlap by 4 nucleotides ybcF and purK are separated by 194 nucleotides
b4572: ylbE - no description, at 547,581 to 548,839
ylbE
b0520: ylbF - hypothetical protein (NCBI), at 548,850 to 549,665
ylbF
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
Position (kb)
549
550
551 Strain fitness (log2 ratio)
-1
0
1 at 548.700 kb on + strand, within ylbE at 548.708 kb on - strand, within ylbE at 548.713 kb on - strand, within ylbE at 548.718 kb on - strand at 548.755 kb on + strand at 548.833 kb on + strand at 549.010 kb on + strand, within ylbF at 549.010 kb on + strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.219 kb on + strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.392 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.458 kb on + strand, within ylbF at 549.488 kb on + strand, within ylbF at 549.491 kb on - strand, within ylbF at 549.534 kb on + strand, within ylbF at 549.625 kb on + strand at 549.695 kb on + strand at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.954 kb on - strand, within ybcF at 550.141 kb on - strand, within ybcF at 550.363 kb on + strand, within ybcF at 550.471 kb on - strand at 550.539 kb on + strand at 550.550 kb on - strand at 550.563 kb on + strand at 550.563 kb on - strand at 550.607 kb on + strand at 550.677 kb on + strand at 550.697 kb on - strand at 550.746 kb on + strand at 550.755 kb on - strand at 550.765 kb on - strand at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK
Per-strain Table
Position Strand Gene LocusTag Fraction pinene 0.3125 vol% remove 548,700 + ylbE b4572 0.89 +1.0 548,708 - ylbE b4572 0.90 -1.5 548,713 - ylbE b4572 0.90 +0.7 548,718 - +0.6 548,755 + -0.2 548,833 + -0.5 549,010 + ylbF b0520 0.20 +1.2 549,010 + ylbF b0520 0.20 -0.6 549,046 - ylbF b0520 0.24 -0.3 549,046 - ylbF b0520 0.24 +0.9 549,219 + ylbF b0520 0.45 +0.1 549,354 - ylbF b0520 0.62 -0.2 549,354 - ylbF b0520 0.62 +0.9 549,392 + ylbF b0520 0.66 +0.4 549,450 + ylbF b0520 0.74 -0.3 549,450 + ylbF b0520 0.74 +0.0 549,458 + ylbF b0520 0.75 -0.4 549,488 + ylbF b0520 0.78 -0.1 549,491 - ylbF b0520 0.79 -0.1 549,534 + ylbF b0520 0.84 +0.1 549,625 + -0.1 549,695 + +0.3 549,755 + ybcF b0521 0.10 -0.3 549,755 + ybcF b0521 0.10 -0.3 549,764 + ybcF b0521 0.11 -0.7 549,811 - ybcF b0521 0.17 -0.5 549,933 + ybcF b0521 0.30 +0.3 549,933 + ybcF b0521 0.30 +1.0 549,954 - ybcF b0521 0.33 -0.4 550,141 - ybcF b0521 0.54 -0.2 550,363 + ybcF b0521 0.78 +1.3 550,471 - -0.1 550,539 + -0.1 550,550 - -0.3 550,563 + +0.0 550,563 - -0.6 550,607 + +0.6 550,677 + +0.4 550,697 - -0.1 550,746 + +0.2 550,755 - +0.4 550,765 - +0.2 550,959 + purK b0522 0.20 +0.7 550,977 - purK b0522 0.21 -0.4 551,057 + purK b0522 0.29 -0.5 551,062 + purK b0522 0.29 +1.0 551,062 + purK b0522 0.29 -0.7 551,091 - purK b0522 0.32 -0.3 551,095 + purK b0522 0.32 -1.3 551,095 + purK b0522 0.32 +0.5 551,112 + purK b0522 0.34 +0.7 551,112 + purK b0522 0.34 +0.7 551,144 + purK b0522 0.37 -0.6 551,162 - purK b0522 0.39 +0.9 551,162 - purK b0522 0.39 -0.8 551,217 + purK b0522 0.44 -0.2 551,227 - purK b0522 0.45 -1.3 551,236 + purK b0522 0.46 +0.2 551,236 + purK b0522 0.46 -0.3 551,255 + purK b0522 0.47 -0.1 551,271 + purK b0522 0.49 +0.5 551,356 + purK b0522 0.57 -0.2 551,375 + purK b0522 0.59 +0.1 551,391 - purK b0522 0.60 +0.4 551,458 - purK b0522 0.66 -1.0 551,532 + purK b0522 0.73 -0.4 551,532 + purK b0522 0.73 +0.5 551,543 + purK b0522 0.74 +0.2
Or see this region's nucleotide sequence