Experiment: Cholinium hydrogen phosphate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgL and ykgO are separated by 34 nucleotides ykgO and ykgM overlap by 1 nucleotides ykgM and ykgR are separated by 363 nucleotides
b0295: ykgL - hypothetical protein (NCBI), at 311,336 to 311,563
ykgL
b4506: ykgO - rpmJ (L36) paralog (NCBI), at 311,598 to 311,738
ykgO
b0296: ykgM - 50S ribosomal protein L31 (NCBI), at 311,738 to 312,001
ykgM
b4671: ykgR - expressed protein (RefSeq), at 312,365 to 312,466
ykgR
Position (kb)
311
312 Strain fitness (log2 ratio)
-1
0
1
2
3 at 310.646 kb on - strand at 310.829 kb on + strand at 310.880 kb on - strand at 310.891 kb on - strand at 310.891 kb on - strand at 310.892 kb on - strand at 310.892 kb on - strand at 310.894 kb on - strand at 310.904 kb on - strand at 311.087 kb on - strand at 311.091 kb on - strand at 311.100 kb on + strand at 311.100 kb on + strand at 311.114 kb on - strand at 311.138 kb on - strand at 311.172 kb on - strand at 311.172 kb on - strand at 311.194 kb on - strand at 311.222 kb on - strand at 311.222 kb on - strand at 311.256 kb on + strand at 311.260 kb on + strand at 311.260 kb on + strand at 311.352 kb on + strand at 311.360 kb on + strand, within ykgL at 311.360 kb on + strand, within ykgL at 311.364 kb on - strand, within ykgL at 311.370 kb on + strand, within ykgL at 311.380 kb on + strand, within ykgL at 311.385 kb on + strand, within ykgL at 311.385 kb on + strand, within ykgL at 311.396 kb on + strand, within ykgL at 311.396 kb on + strand, within ykgL at 311.402 kb on + strand, within ykgL at 311.402 kb on + strand, within ykgL at 311.418 kb on - strand, within ykgL at 311.470 kb on + strand, within ykgL at 311.470 kb on + strand, within ykgL at 311.525 kb on + strand, within ykgL at 311.626 kb on + strand, within ykgO at 311.626 kb on + strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.722 kb on + strand, within ykgO at 311.722 kb on + strand, within ykgO at 311.829 kb on + strand, within ykgM at 311.829 kb on - strand, within ykgM at 311.944 kb on - strand, within ykgM at 311.977 kb on + strand at 311.985 kb on - strand at 312.124 kb on + strand at 312.124 kb on + strand at 312.125 kb on + strand at 312.144 kb on + strand at 312.146 kb on + strand at 312.146 kb on + strand at 312.215 kb on + strand at 312.216 kb on + strand at 312.258 kb on + strand at 312.258 kb on + strand at 312.260 kb on + strand at 312.269 kb on + strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.490 kb on - strand at 312.505 kb on - strand at 312.520 kb on - strand at 312.522 kb on - strand at 312.570 kb on + strand at 312.586 kb on + strand at 312.596 kb on + strand at 312.605 kb on + strand at 312.615 kb on - strand at 312.619 kb on + strand at 312.642 kb on - strand at 312.648 kb on + strand at 312.648 kb on + strand at 312.650 kb on + strand at 312.658 kb on - strand at 312.660 kb on + strand at 312.660 kb on + strand at 312.664 kb on + strand at 312.666 kb on + strand at 312.672 kb on - strand at 312.674 kb on - strand at 312.677 kb on + strand at 312.679 kb on - strand at 312.679 kb on - strand at 312.690 kb on - strand at 312.690 kb on - strand at 312.693 kb on - strand at 312.695 kb on + strand at 312.712 kb on - strand at 312.721 kb on + strand at 312.721 kb on + strand at 312.721 kb on + strand at 312.729 kb on + strand at 312.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium hydrogen phosphate 10 vol% remove 310,646 - +1.6 310,829 + +1.6 310,880 - +1.6 310,891 - +1.6 310,891 - +1.6 310,892 - +1.6 310,892 - +0.6 310,894 - +1.6 310,904 - +0.0 311,087 - +1.6 311,091 - -0.4 311,100 + +0.6 311,100 + +0.8 311,114 - +0.0 311,138 - -1.6 311,172 - +0.0 311,172 - -1.0 311,194 - -0.3 311,222 - +2.4 311,222 - -1.0 311,256 + -0.3 311,260 + +0.1 311,260 + -0.1 311,352 + +0.0 311,360 + ykgL b0295 0.11 +0.9 311,360 + ykgL b0295 0.11 -0.2 311,364 - ykgL b0295 0.12 +0.7 311,370 + ykgL b0295 0.15 -0.0 311,380 + ykgL b0295 0.19 -0.7 311,385 + ykgL b0295 0.21 -0.0 311,385 + ykgL b0295 0.21 +0.1 311,396 + ykgL b0295 0.26 +0.4 311,396 + ykgL b0295 0.26 +1.0 311,402 + ykgL b0295 0.29 -0.2 311,402 + ykgL b0295 0.29 +0.2 311,418 - ykgL b0295 0.36 +1.6 311,470 + ykgL b0295 0.59 -0.8 311,470 + ykgL b0295 0.59 +0.3 311,525 + ykgL b0295 0.83 +1.6 311,626 + ykgO b4506 0.20 +0.6 311,626 + ykgO b4506 0.20 +2.6 311,634 - ykgO b4506 0.26 +0.9 311,634 - ykgO b4506 0.26 +0.3 311,634 - ykgO b4506 0.26 +0.6 311,722 + ykgO b4506 0.88 +0.6 311,722 + ykgO b4506 0.88 +1.6 311,829 + ykgM b0296 0.34 +0.6 311,829 - ykgM b0296 0.34 +0.2 311,944 - ykgM b0296 0.78 -0.6 311,977 + -0.1 311,985 - +0.2 312,124 + +0.3 312,124 + +0.4 312,125 + -0.4 312,144 + +0.0 312,146 + -0.3 312,146 + -0.8 312,215 + +0.0 312,216 + +0.8 312,258 + +0.7 312,258 + -0.1 312,260 + -1.2 312,269 + -0.1 312,488 - -0.4 312,488 - -0.5 312,488 - -0.4 312,488 - +0.5 312,490 - -1.7 312,505 - -0.2 312,520 - +0.3 312,522 - +0.4 312,570 + +0.3 312,586 + -1.2 312,596 + -0.2 312,605 + -0.7 312,615 - +1.2 312,619 + +1.6 312,642 - +2.4 312,648 + -1.4 312,648 + +2.6 312,650 + +3.2 312,658 - +0.6 312,660 + -0.4 312,660 + +1.1 312,664 + -0.3 312,666 + +0.0 312,672 - +1.6 312,674 - +1.6 312,677 + +1.0 312,679 - +1.6 312,679 - +0.6 312,690 - +1.6 312,690 - +1.6 312,693 - +1.6 312,695 + +0.1 312,712 - +2.2 312,721 + -0.4 312,721 + +2.3 312,721 + +0.5 312,729 + +1.6 312,731 - +1.6
Or see this region's nucleotide sequence